EMAGE mouse gene expression spatial database

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EMAGE:14734

Klhl18 kelch-like 18 (Drosophila) ( MGI:2143315)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005777. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005777_01	euxassay_005777_02	euxassay_005777_03	euxassay_005777_04

euxassay_005777_05	euxassay_005777_06	euxassay_005777_07	euxassay_005777_08	euxassay_005777_09

euxassay_005777_10	euxassay_005777_11	euxassay_005777_12	euxassay_005777_13	euxassay_005777_14

euxassay_005777_15	euxassay_005777_16	euxassay_005777_17	euxassay_005777_18	euxassay_005777_19

euxassay_005777_20	euxassay_005777_21	euxassay_005777_22	euxassay_005777_23	euxassay_005777_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14734_wholemount_strong.wlz

14734_wholemount_moderate.wlz

14734_wholemount_weak.wlz

14734_wholemount_possible.wlz

14734_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14734_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

weak

regional

weak expression: see section 20 21

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 07 18

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 09 17 18 19 20 21 22

vagus x ganglion

weak

regional

weak expression: see section 08 17

spinal cord

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15

dorsal root ganglion

weak

regional

weak expression: see section 07 08 09 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2756
Entity Detected:	Klhl18, kelch-like 18 (Drosophila) ( MGI:2143315)
Sequence:	sense strand is shown >T2756 GGCCTCNAGNCAGATTCGGACGAGGTGCTTTCCTAAAATCCTTCCAGTAGCGACGAGAAGGATATAATTG TTTCTAGCCCCAAACTGGGTCTTAGGGTCCGGTCTGTTCCACTGTCCACCTGATTGGTTTCTTGCCCTCT GCTGCCTGCCTGGATCGGCCTAGAAGACGGTTCAGGCGGCTCTGGGTGAAGCCTGCGGGGGCAGGGGGGT GGTCCCTTTCCCCAGACCATCAGGTTTCCCAGTGTGTGCAATTGCCATAGATTCAGAAGGAAGGACTCTT GTTCCCTGCAATGTAAAGAGCCGGGCCTCTGCTCTAAGCACTTGAAAATAATGTTTTTATCTTAAAAGAC TGAACAATCTGGTAACTGATACATCTTAACTAGGCTCCCAGTGGAGCGCCAGCATTTCTTCCTGCCCCTC AGTTTGCCTGCTGAGGTAGACTCTAGGGGACTATGGGTCTTGGTTTCCTCTTAGTCCTATTAGGAGGGGT GTATCCACGCTCCTCTTATTAAGTCCTTCTGTGAATGTACTGCCCAGCCTCGACTGTGAAGGTGCTGAGA ACCA
Notes:	The probe template was PCR amplified from IMAGE:1514461 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1514461 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14735 same embryo
	EMAGE:14733 same embryo
	EMAGE:14732 same embryo
	EurExpress:euxassay_005777 same experiment
	MGI:4825795 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source