EMAGE mouse gene expression spatial database

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EMAGE:14789

Cd5l CD5 antigen-like ( MGI:1334419)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005723. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005723_01	euxassay_005723_02	euxassay_005723_03	euxassay_005723_04

euxassay_005723_05	euxassay_005723_06	euxassay_005723_07	euxassay_005723_08	euxassay_005723_09

euxassay_005723_10	euxassay_005723_11	euxassay_005723_12	euxassay_005723_13	euxassay_005723_14

euxassay_005723_15	euxassay_005723_16	euxassay_005723_17	euxassay_005723_18	euxassay_005723_19

euxassay_005723_20	euxassay_005723_21	euxassay_005723_22	euxassay_005723_23	euxassay_005723_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14789_wholemount_strong.wlz

14789_wholemount_moderate.wlz

14789_wholemount_weak.wlz

14789_wholemount_possible.wlz

14789_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14789_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver

strong

single cell

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7233
Entity Detected:	Cd5l, CD5 antigen-like ( MGI:1334419)
Sequence:	sense strand is shown >T7233 CTAGTGTTCAAACCAAATACCACTGCCTGAGGCCTACAGCCTGTCTCACCACTCCATTGTCAGCTGCCTG GGCCATGGCTCCATTGTTCAACTTGATGCTGGCCATCTTGAGCATTTTTGTTGGATCGTGTTTTTCAGAG TCTCCAACCAAAGTGCAGCTAGTGGGAGGTGCCCACCGCTGTGAAGGGCGAGTGGAGGTGGAACACAATG GCCAGTGGGGGACTGTGTGTGATGATGGCTGGGACCGGCGTGATGTGGCTGTGGTGTGCCGAGAGCTCAA TTGTGGAGCAGTCATCCAAACCCCGCGTGGCGCATCATATCAGCCACCAGCATCAGAGCAAAGAGTTCTT ATTCAAGGGGTTGACTGCAACGGAACGGAAGACACGTTGGCTCAATGTGAGCTAAATTACGATGTTTTTG ACTGCTCACATGAAGAAGATGCTGGGGCACAGTGTGAGAACCCAGACAGTGACCTCCTCTTCATTCCAGA GGATGTGCGTCTAGTAGATGGCCCGGGGCACTGCCAGGGTCGAGTGGAGGTGCTCCACCAGTCCCAGTGG AGCACTGTGTGTAAAGCAGGCTGGAACTTACAGGTCTCAAAGGTGGTGTGCAGGCAGCTCGGGTGTGGGC GGGCATTACTGACCTACGGAAGCTGCAACAAGAGTACTCAGGGCAAAGGACCCATCTGGATGGGCAAGAT GTCGTGTTCTGGACAAGAAGCAAACCTTCGGTCTTGCCTTTTGAGTCGTTTGGAGAACAACTGTACCCAT GGCGAGGACACATGGATGGAATGTGAAGATCCTTTTGAGCTGAAGCTGGTGGGAGGAGACACCCCCTGCT CTGGGAGGTTGGAGGTGCTGCACAAGGGTTCCTGGGGCTCCGTCTGTGATGACAACTGGGGAGAAAAGGA GGACCAAGTGGTCTGCAAGCAACTGGGTTGTGGGAAGTCCCTCCATCCATCCCCCAAAACCCGGAAAATC TATGGGCCTGGGGCAGGCCGCATCTGGCTGGATGACGTCAACTGCTCAGGGAAGGAACAGTCTCTGGAGT TCTGCCGGCACAGGTTGTGGGGGTACCACGACTGTACCCACAAGGAAGATGTGGAGGTGATCTGCACAGA CTTTGATGTGTGAATTGGATCCCTGCTTGTTCAGTGGGCCCTCATTCTCCCAGTGGTTACATCAGGCTGT GGGCTTTAGACACCTTTCCCTCAGCCTCGAAAGAGTCTGAACATTGTGTTCCTATCTTGATCTCAAGGCT ACACGCCCCCATAATCACCTCAAGACATGAGCTGCTGAGCTCCCTTGCTGACCTTTCCAGCTGCCCTAGG CTCACTGTTCACTCCTTGGTGAAAAGCCCCCACCTTTACTGTCTCTCCCCAGCCTGCCTGCAACTCTTGG GCCTGCCAGAGTGAGCAGCTGTACAGGCCAGGAGTAAGACACGGCCTGTCTGTGAACACCACTGAGGATG TGACAACATGAGGAACACTTGAGAGGGAATGTGGGTAGACAGATTCTTGGAGGCGGGAGAGATAATACAA TTGTTTAAATGCTTTTTAAACTTTGTAACAAGTGAAGTGATCATAATAATAACACTTCACTACTCTGCTT CTCTCAGAGAAAGCAGCAGGGTGGTTTCCTGCAGCCCTCAAATGTTACCTGTTGAGTTCTAGATGTCTAC CCCAAACCTCCATGTTTAAAGTTTGATGTCTAATGCAACAGTATTCTGAGGTGGGGCCTTAGGGATCTAA CTGCATCATGTGGTTTGATCCCTCAGTCTTTATGAGTGGATTAATCACTGGTGAATCCCCTGGGAGATGG TAGAGACTTGAGGAGTTGGGACCTAGTTAGAAGAAGAAGGTCGTTGGAGTGTGCCTTTGGAAGGGGCTGT TTTGTCCACAGCTTGACCACCTCCCTGTGTAGATGGGTCGCTTGCATATCTTCTCTACTGTGTGTGAATT ATGCTACAATAAACATGAGTGCTCAAGTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4161650 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4161650 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14790 same embryo
	EMAGE:14788 same embryo
	EMAGE:14792 same embryo
	EMAGE:14791 same embryo
	EurExpress:euxassay_005723 same experiment
	MGI:4823739 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source