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EMAGE:14793

Rbpjl recombination signal binding protein for immunoglobulin kappa J region-like ( MGI:1196616)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14793 EMAGE:14793 EMAGE:14793 EMAGE:14793 EMAGE:14793
"Pseudo-wholemount" of euxassay_005797. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005797_01 euxassay_005797_02 euxassay_005797_03 euxassay_005797_04
EMAGE:14793 EMAGE:14793 EMAGE:14793 EMAGE:14793 EMAGE:14793
euxassay_005797_05 euxassay_005797_06 euxassay_005797_07 euxassay_005797_08 euxassay_005797_09
EMAGE:14793 EMAGE:14793 EMAGE:14793 EMAGE:14793 EMAGE:14793
euxassay_005797_10 euxassay_005797_11 euxassay_005797_12 euxassay_005797_13 euxassay_005797_14
EMAGE:14793 EMAGE:14793 EMAGE:14793 EMAGE:14793 EMAGE:14793
euxassay_005797_15 euxassay_005797_16 euxassay_005797_17 euxassay_005797_18 euxassay_005797_19
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euxassay_005797_20 euxassay_005797_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14793Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14793_wholemount_strong.wlz
14793_wholemount_moderate.wlz
14793_wholemount_weak.wlz
14793_wholemount_possible.wlz
14793_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14793_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
pancreas
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35523
Entity Detected:Rbpjl, recombination signal binding protein for immunoglobulin kappa J region-like ( MGI:1196616)
Sequence:sense strand is shown

>T35523
GCGTTACCTCTCTGTGGAAGATGGGGCCTTTGTGGCCAGCGCAAGGCAGTGGGCTGCCTTCACCCTCCAC
CTGGCTGATGGGCACTGTTCCCAAGGGGACTTCCCACCCCAGGAAGGTTACATCCGCTACGGCTCCCTGG
TGCAGCTTGTCTGCACTGTCACAGGCATTACACTGCCGCCTATGATCATCCGAAAAGTGGCCAAGCAGTG
TGCCCTCCTTGATGTGGATGAACCCATATCCCAGCTGCACAAGTGTGCCTTCCAGTTTCCAAGTGACACC
CCAGGAGGGGCTGGCACCTACTTATGCCTTGCCACAGAGAAGGTGGTGCGATTCCAGGCTTCCCTCTGCC
CCAAGGAGGCCAACAGGGCGCTGCTCAACGACAGCTCTTGCTGGACCATCATTGGCACGGAGTCGGTGGA
GTTCACCTTCAGCACCAGCCTGGCCTGTACTCGGGAGCCCGTCACTCCGGTGCCTCTCATCAGCACTCTG
GAGCTGAGCGGTGGAGGTGATGTGGCCACGCTGGAGCTGCACGGAGAAAATTTCCACGCTGGGCTCAAGG
TGTGGTTCGGAGAC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 98459. Forward Primer - name:098459_F_cDNA_Rbpsuhl, sequence:GCGTTACCTCTCTGTGGAAGAT; Reverse Primer - name:098459_N_SP6_cDNA_Rbpsuhl, sequence:GTCTCCGAACCACACCTTGAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14794 same embryo
 EMAGE:14797 same embryo
 EMAGE:14798 same embryo
 EMAGE:14796 same embryo
 EMAGE:14795 same embryo
 EurExpress:euxassay_005797 same experiment
 MGI:4827659 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS