EMAGE mouse gene expression spatial database

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EMAGE:14828

Nln neurolysin (metallopeptidase M3 family) ( MGI:1923055)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005807. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005807_01	euxassay_005807_02	euxassay_005807_03	euxassay_005807_04

euxassay_005807_05	euxassay_005807_06	euxassay_005807_07	euxassay_005807_08	euxassay_005807_09

euxassay_005807_10	euxassay_005807_11	euxassay_005807_12	euxassay_005807_13	euxassay_005807_14

euxassay_005807_15	euxassay_005807_16	euxassay_005807_17	euxassay_005807_18	euxassay_005807_19

euxassay_005807_20	euxassay_005807_21	euxassay_005807_22	euxassay_005807_23	euxassay_005807_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14828_wholemount_strong.wlz

14828_wholemount_moderate.wlz

14828_wholemount_weak.wlz

14828_wholemount_possible.wlz

14828_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14828_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4180
Entity Detected:	Nln, neurolysin (metallopeptidase M3 family) ( MGI:1923055)
Sequence:	sense strand is shown >T4180 CCCACGCGTCCGCTGGTCTGCTCCGCTCTGCCCGCTCTCGGCTTCCGGGCTCGCAGACTCCACCGCCCGC CTCCTGCACCTACCAAGCCGGAGGACCACGCGCCTGCTGCTGCCGGCCTCCCGGCCTGCCCATGATCACC CTGTGCCTTTCGGCTCTGCGAGGCCTCCACAGAGCTGGTGGTTCCAGGATTCGGCTCAGAATGACGTTGG GGAGGGAGGCGGCATCTCCTCTTCAGGCCATGTCTTCCTACACCGCCGCAGGCAGGAATGTTCTCAGATG GGACCTTTCTCCAGAGCAGATCAGGACGAGGACAGAGGAGCTCATAGCACAGACCAAGCAGGTGTATGAT ACCGTGGGGACAATCAACCTGGAGGATGTAACTTACGAGAACTGTCTGCAGGTGCTGGCTGACATAGAAG TGAAGTACATAGTGGAAAGGACCATGCTGGACTTCCCTCAGCACGTGTCGTCTGACAGAGAAGTGCGGGC TGCAAGCACAGAAGCGGACAAAAGGCTTTCTCGTTTTGATATTGAGATGAGCATGAGAGAAGATGTATTT CAGAGAATTGTTCATTTACAAGAAACGTGCGATTTGGAGAAGATAAAGCCTGAGGCCAGGCGATACTTGG AAAAATCAATTAAAATGGGGAAAAGGAATGGACTCCATCTTCCAGAACACGTAAAAAATGAAATCAAATC AATGAAGAAGAGAATGAGTGAGCTATGCATTGACTTCAACAAAAACCTCAATGAGGATGACACGTCCCTT GTATTTTCCAAGGCTGAACTTGGTGCTCTTCCCGACGACTTCATTGACAGTTTAGAAAAAACAGATGAAG ACAAGTACAAAGTTACCTTAAAGTATCCCCACTATTTTCCTGTCATGAAGAAATGCTGTGTCCCTGAAAC TCGAAGGAAGATGGAAATGGCTTTTCATACCAGGTGCAAAGAGGAAAACACCATAATTCTGCAACAGCTC CTGCCGCTGCGAGCACAAGTGGCCAAACTGCTCGGCTACAACACCCATGCTGACTTTGTCCTTGAACTGA ACACTGCAAAGAGCACAAGCCATGTGGCAACCTTTCTAGATGATTTAAGCCAGAAATTAAAACCCTTGGG TGAGGCTGAACGGGAGTTTATTTTGAGCTTGAAGAAGAAGGAATGCGAAGAGCGAGGTTTTGCATACGAC GGGAAGATCAACGCATGGGACCTCCATTACTACATGACCCAGACAGAGGAGCTCAAGTACTCTGTGGACC AGGAGTCCCTCAAGGAGTACTTTCCCATTGAGGTGGTCACTGAAGGCCTGCTAAGCATCTACCAGGAGCT GTTGGGACTTTCATTTGAACAAGTGGCTGATGCCCATGTTTGGAATAAGAGTGTTTCACTTTACACCGTG AAGGATAAAGCTACTGGAGAAGTGCTGGGGCAGTTCTACCTGGACCTCTATCCAAGGGAAGGAAAATACA ACCATGCGGCCTGCTTTGGTCTCCAGCCAGGCTGCCTTCTCCCTGATGGCAGTCGGATGATGTCTGTGGC TGCCCTGGTGGTCAACTTCTCTCAGCCCATAGCAGGCCGGCCCTCTCTCCTGAGACACGACGAAGTGCGA ACTTACTTCCATGAGTTTGGTCACGTCATGCATCAGATCTGTGCACAGACTGACTTTGCACGATTTAGTG GAACAAATGTGGAAACTGACTTTGTAGAGGTGCCATCACAAATGCTTGAAAACTGGGTGTGGGACATTGA CTCCCTGCGAAAACTGTCAAAACATTATAGAGACGGACACCCTATCACAGACGAGCTGCTGGAGAAGCTT GTGGCTTCGAGACTGGTCAACACAGGTCTTCTGACGCTTCGCCAAATTGTTTTGAGCAAAGTTGACCAGT CCCTCCATACCAATGCGTCGCTGGATGCCGCGAGCGAATACGCTAAATACTGCACAGAAATTTTGGGAGT TGCAGCTACGCCAGGCACAAATATGCCAGCTACCTTTGGACATTTGGCAGGAGGGTATGACGGCCAGTAT TATGGATATCTTTGGAGTGAAGTCTTTTCCATGGACATGTTTCACAGCTGTTTCAGAAAAGAAGGGATTA TGAATCCAGAGGTTGGAATGAAATACAGAAACCTAATCCTGAAGCCTGGGGGGTCCCTGGACGGCATGGA CATGCTCCAGAATTTCTTGCAACGTGAGCCAAACCAGAAAGCATTCCTGATGAGTCGAGGCCTGAATGCT TCGTAAAGCAGGGACGCTGGGGGCTGTCCGTGGCTGGAGGACAAGCCAACACCGTCCGCCATGTGGTGCC GGCCTGGAGACTGAAGGCAGTTTTACAAAGGAAAACTTAGATTTCTATTCATTTGCTTTTGTTTCTTCAT TTTAAAAATTATACAGATGTAAATGGAATTATAAATACTGTGACCTAAGAAAAGACCCACTAAAAATAAT TGTACTATAAAGTTTCATAAAAACAGATTTGATTTCTTTTGATAAAAGTTTCACATGACTGTAATTTGAT TTTTTTACTGTTATAATCCAGATAATATAATGTAAGAGGGCTAAGACATTTTAAATTGAATCACATACAT GATATAATTTGATCTTTCTTATATCTTGAAGTTTTATACTTGAGATTCCTGGACTGATAAGTGAATCAGC ATGTTCTCTCTGGTAAATATTTTCTCAGAGCCCTGTGTCAACCTTGACCCTCTGCCTCACAACTAAGTGA CAGTTTGCCTGTGTGTGTCAAGGCCCGCAGGATACACTTCAATGCTGTTTGTCTTTTGTAGTGAGCTTTG TGATTCTCGGCTTCTGTCACACTTACTTACATAGCTCCACTTAGATTTGGGGTTCTTTCCCTTCTAAAAT TTAATTTCTAAAGTTAATATTATTATTTAGATAATATTTTTAAATTTTGTTTCAACTGGCCTAGTATATA AGTTTTGTGTCTTGCACTAGAAGCTCAAATGTCAAGTCTTCGGTTGCAGTCACAGTGAGAGAAGCAGAGA ACTTAGGCATCTTTTGGGTTCTCGACTTTTAGTATTTGCTATGTATTTGTTTCTGCTTGTTTTGCTTATA TATATATATTTTTTTTATTTTGTTTGCTTGAGGCACCATCTCACCATGGAGCCAGATTCGCCTGGAACTC ATGGTAATTCCCCTGCTTCGGCCTCCCAAGCCTTAGGGGCGCAGGTGTGAGCTGCCTCACTCAGCTCAGT TCTCACGTTTCTAATAAGACTCTGACCTTGCCTCCCTGAAAGTGGTAGGTCGGCCCTGTAGTCACAGTGT GTTGAAGTGCCAGGGGGAGAGATGACATCCACTGGAACTTAGCTTTGAAAACTCCAGAAAAGAGTGCGTT GGAGCAGAGCGTTTGCGAACAGCTGCTCCCAAGTGTTCCGTGGGCAACCGAGCTGTGGGAAAAGGCTGTC CAGCAGGCTGTTTCATATGGCGACTGTTAAGTGAACCTCCAGATCTCCAACTCCTTTTATTGGGACCTAA GTTTCCAGCTCAGAGTAGTGAGCTCACAGCATTATTTTAGCCTTGGGAGTCTACTCCAAACGGAAGCCAA GTAGGATAGAGAGGAGAAAATGTAAATGAGCTGTGATCGGACTTTCCACAGGGCTTTGAGTTGTTTCTCC ACACATACCTGAGGAATGACTTTCCTGGCAGGAAACGAAATTAGAGCCTGTCTGTTCTTTAGACCCGGTG GACTTTGTGTTCAATAAAATATTATGTCGTGTAATAAAGTTATACAGAGTACCAAGAGCTGGTACACAGC AGGGAAGCCCTTTGTTTATGTAGTTTGATTTGATTTTTACAAGGCTGGGCATCAAACCTCCGGCTTCTTC ACCCATGCTAGCAAGTGCTATGCTCTCCCAGCTGCTTTTATGGTGGTGGTAGAAACCTCTGGAAGCGTCT CACATCTGTCCCCACCCCCATTTTTGTTTCCTACTTTCAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAA A
Notes:	The probe template was PCR amplified from IMAGE:4486941 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4486941 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14827 same embryo
	EMAGE:14830 same embryo
	EMAGE:14831 same embryo
	EMAGE:14829 same embryo
	EMAGE:14826 same embryo
	EurExpress:euxassay_005807 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source