EMAGE mouse gene expression spatial database

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EMAGE:14863

0610007P08Rik RIKEN cDNA 0610007P08 gene ( MGI:1923501)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005815. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005815_01	euxassay_005815_02	euxassay_005815_03	euxassay_005815_04

euxassay_005815_05	euxassay_005815_06	euxassay_005815_07	euxassay_005815_08	euxassay_005815_09

euxassay_005815_10	euxassay_005815_11	euxassay_005815_12	euxassay_005815_13	euxassay_005815_14

euxassay_005815_15	euxassay_005815_16	euxassay_005815_17	euxassay_005815_18	euxassay_005815_19

euxassay_005815_20	euxassay_005815_21	euxassay_005815_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14863_wholemount_strong.wlz

14863_wholemount_moderate.wlz

14863_wholemount_weak.wlz

14863_wholemount_possible.wlz

14863_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14863_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9609
Entity Detected:	0610007P08Rik, RIKEN cDNA 0610007P08 gene ( MGI:1923501)
Sequence:	sense strand is shown >T9609 AAATCTCTTGTGTCTCGAGAACATGACCTCAAAATCTGTTAGGAAAAGAAAAGGTACAGATGATATTAGC GATGAGTCTGATGACATTGACATGTTCCCCAAGTCAAGAATAAGAAAGCAAAGAGCCACTACTTCCTTGA AATTTAAGAGAAAAAAGGAAAACAAAAGGAAACTTGATAATTCTCCTGTAACAGCAAAGGAAGCAAATCA AGTGTGTGCAGCTGATGGAGACCGTAGCTCTCAGGTTATTGAGGATTTTTCATCCTCAGATGACAATTTA TCTCTCAGCCACTTGAGTTTCACTAAACTAAGTCACAGAGCAGAAACTGTAAAAGATAAAATCAGCTTGT CCCCCAAGCTGCCTGGCCCAGATAAGAAAAATAACACTTTTATATCAAGAAAACCACCAAGCTTTCTAAA TGAAGGAGTTATCAGTCAAGAGCAAATATGCAACTCAATGGATAAAATATTAGATGGTGTTCAGGAAGTG GCTTATATCCACTCAAATCAGAATGTGATCGGCTCAAGCAGAGCTGAGAACCACATGAGCCGATGGGCAA CGCGTGATGTGTTTGAGTTGAAGCAGTTTTCCCAGCTCCCTGCTAATGTAGCTGTTTGCAGTTCTAAGAC ATACAAAACTCAGGTGAAGGCAAACATAGTGTCACCTACAGAGAAAGATCAACCACCAAGTGATGGAGGC ATTTCCTCTCCTCTGTACGTATCCCACCCTGTAGTCCAGAAAAAGAAAGATGTATATCGTACTAACCATA CTACCTTCATCATTGGAGAAACACCAAGAGGAATCCGCAGAAAGCAGTTTGAAGAAATGGCCTCTTACTA TAAGCTGCCTGTAAAGGAGTTTGCTGAACAAGTTACTAGGGCCACATCTGAAGAGCGACAGAAAATGCTC AGAGACTTCTACAGTCTTCAGCACCCAGAGGTAAAGGAGTTCTTTGTGAATTCTGCTTCAGAATTGATAA AATCTGTCCACAAGAAAGAAGAGAGGGTGAGGAATAAATCTAAAGAAAAAGAATCTCTTTTAAAAGAAAA CCCCTCAAATGATAGTACTTTATCATGTTATGATTCCACCAACAAAATGTCTCAAGTTTACAACAGAAAA ATATGTGAAGGAAAATCAGTCAGATCTCAGAATCATGTTTTCCATAGAGAAGACACATTTTCTAGTGATG CAGAGATTAACAAATCACCTGTCAGCTTTACTGAAGAGCTTCACAGTGAGAGAAAGGACCACACACCCAA GGATACCACAACAGTCTTCTGTCCAAACAGCAACTCTGAAGCCCTGGAGGCAGAGCTAGGAAATTCCCCA GGACGTCAGTGGGACCTCACAGGAGCATGCGGTTCAAGAAACAGACCACTTTTCAAGTTGAGAAACAAAA GGGTAGAAAACCCAGGGTCAGAAAACACTCCTGAGGATGGCTTACTTGGTGACACCTCTATTCTTAACGA CCTCTTTAAAAGCCACGGGGAAGGTCCCACACAGCTGCCAAAGAATGTTCTTTCAGGACCTGTGGCAAAA GCAAAGCAGAAACCAAAAGATTTCTGGGACATCTTGAATGAGCAGAATGATGACAGTCTCAGCAAACTCA CTGACTTGGCAGTGATAGAGACGCTGTGTACCAAAGCACCCAGTACCTCAGCCTCCAAAAGGAAAGATGA GCTGGAAGCTTCCCTTTGGAAAGCAAACGAGAAGTTTTTGTGGAAGACATTAAGCTCAGATGTTGACGAT GAAAGCATAAGCAATACAGAGAGAGAGTAGGACATGGACGTGCCATTAACCTAGATATTGATGAGCTGCT GGCCCTGGCTATGGATGTTCATGTATGGGGCACACTGAACATTTGTTTACAAACTTGTGTATTAAATTAT TGCTTTAGTATAATCTAAACAGCCTAGCATGTTATCATGGGTTCTTTCCCTAATATCTGTTTTAATCATT AACGCTCATGTATGTAGTTTATGTAGTACTGGAGGTCAAGCCCAGGACTTTATGCAAGCACTCTGGGCAA CTGAGCTACATCTCCTGCCCCAGATTTGGTCTTCAGAACAAATGTAATTGTATCATATTGTAACGTGTCA TAATATAACTATTATTAACTGGCATCTTCCCTCCTGTGTGAGTCCGTGGCCTTACGTGAGTGACTTGCCC ATTCATTATCTGAAAGATAACCGACACACAGCCTTCCACTTTTAAACTCCAGAATGCCCCAAAGTCAAGT GGCAGCCACTGCCCTCTCCTCATTGGCATTGGGAACTTACTGTTTTCCTTCTTCCTTGGAATTATGTTCT ATTACTATTAAATTATTTTAGATAAAAGTTATAATGAAGACTCAACAATTAGGACTCAATGTATGGCATT ATGTTAAAAATGCTAATTTTTATAATCACTCAGAGTTTGTGTGCTGTGGTCTAAAGAAATGGGATTTTTA TCTTTGCCCAGGATTCCGATGCCTTAACTTTTCACATTACTATGATTTAGTAAATATAAATAGATTTATA GCCTAACTTTGTATTTCTTAAGTAAAAGGAAATGAATAAACATTTGATTTTAATTGAAAAAAAAAAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30649253 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30649253 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14864 same embryo
	EMAGE:14862 same embryo
	EMAGE:14861 same embryo
	EMAGE:14860 same embryo
	EurExpress:euxassay_005815 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source