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EMAGE:14874

Zfp39 zinc finger protein 39 ( MGI:99183)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14874 EMAGE:14874 EMAGE:14874 EMAGE:14874 EMAGE:14874
"Pseudo-wholemount" of euxassay_007923. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007923_01 euxassay_007923_02 euxassay_007923_03 euxassay_007923_04
EMAGE:14874 EMAGE:14874 EMAGE:14874 EMAGE:14874 EMAGE:14874
euxassay_007923_05 euxassay_007923_06 euxassay_007923_07 euxassay_007923_08 euxassay_007923_09
EMAGE:14874 EMAGE:14874 EMAGE:14874 EMAGE:14874 EMAGE:14874
euxassay_007923_10 euxassay_007923_11 euxassay_007923_12 euxassay_007923_13 euxassay_007923_14
EMAGE:14874 EMAGE:14874 EMAGE:14874 EMAGE:14874 EMAGE:14874
euxassay_007923_15 euxassay_007923_16 euxassay_007923_17 euxassay_007923_18 euxassay_007923_19
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euxassay_007923_20 euxassay_007923_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14874Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14874_wholemount_strong.wlz
14874_wholemount_moderate.wlz
14874_wholemount_weak.wlz
14874_wholemount_possible.wlz
14874_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14874_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35576
Entity Detected:Zfp39, zinc finger protein 39 ( MGI:99183)
Sequence:sense strand is shown

>T35576
CATAAGTCCTCCCTCACTGTCCATCAGAGAAGCCACACAGGCTACAAGCCATACTCGTGTGAGGAGTGCA
GGAAAACGTTTTACAGTAAGTCACATCTCACCGTCCATCAGAGAACCCACACAGGCGAGAAGCCCTATGA
GTGTAAGCTATGCAAGAAGGCTTTTCACCAGAAGTCATACCTTAACCGGCATCAGGTAACTCACGGAAGT
GAGAAACGATTTGAATGTCAAGAATGTAGGAAAACGTTTTATCACAAGTCTTCCCTCACTGTACATCAGA
GAATCCACCTGAGGGAGCTCCTGTGCGTCTGATGTGGGAAGCTCTGTCAGCAGGAACCATCAGCTGGCAC
CAGACAAGGCATGTGGAGGGCGTCTCCTATAAATGTGACAGTGTGGACAATGTTTTTGCAAGAAAGGACA
CCTTTGCCAAGTGCTAACTTTACACAGGAAATTCTGTGTGGCACAAGTTGGCTGTCTTCTCTGGAACATA
GTCCCCATTACTGAGGGTCTGTGTGATCATGTAACACACACACACACACACACACACACACACACACACA
CACACACACACACACACACACACTGCTTTCATTTCACTGCTTTCAGCCAGGCCCACAGAAATACTTTTTT
CTTCCTCCCTAAGTTCTCATGAGCTCTGGGTCTGAGATAAATGTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 64295. Forward Primer - name:064295_F_cDNA_Zfp39, sequence:CATAAGTCCTCCCTCACTGTCC; Reverse Primer - name:064295_N_SP6_cDNA_Zfp39, sequence:GACATTTATCTCAGACCCAGAGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14875 same embryo
 EMAGE:14872 same embryo
 EMAGE:14871 same embryo
 EMAGE:14876 same embryo
 EMAGE:14873 same embryo
 EurExpress:euxassay_007923 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS