EMAGE mouse gene expression spatial database

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EMAGE:14894

Rd3 retinal degeneration 3 ( MGI:1921273)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007914. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007914_01	euxassay_007914_02	euxassay_007914_03	euxassay_007914_04

euxassay_007914_05	euxassay_007914_06	euxassay_007914_07	euxassay_007914_08	euxassay_007914_09

euxassay_007914_10	euxassay_007914_11	euxassay_007914_12	euxassay_007914_13	euxassay_007914_14

euxassay_007914_15	euxassay_007914_16	euxassay_007914_17	euxassay_007914_18	euxassay_007914_19

euxassay_007914_20	euxassay_007914_21	euxassay_007914_22	euxassay_007914_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14894_wholemount_strong.wlz

14894_wholemount_moderate.wlz

14894_wholemount_weak.wlz

14894_wholemount_possible.wlz

14894_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14894_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

choroid invagination

moderate

regional

moderate expression: see section 07 08 09 10 11 15 16 17 18

metencephalon part of 4th ventricle choroid plexus

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17

vomeronasal organ

moderate

regional

moderate expression: see section 11 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35611
Entity Detected:	Rd3, retinal degeneration 3 ( MGI:1921273)
Sequence:	sense strand is shown >T35611 AAGCAGGACCCCTACAGTGATAGTGGCCCTGAAGGAGGTGGACCTAAGAAGAGTCTGGGGGGCCATGTCC CTCATCCCGTGGCTCCGGTGGAACGACACCCCTCCCCGGCTCTCGGCCCGGACCCCGGCCGAGATGGTGC TGGAGACGCTCATGATGGAGCTGGCTGGGCAGATGAGAGAGGTGGAGCGACAACAGAGGGAGCGTCGCAG CGCAGTCAGGAAGATCTGCACTGGTGTGGACTACAGCTGGTTGGCAAACACGCCCAGGCCCACCTATGAC ATCAGCCCTGGTGAGCGGCTGCAGTTGGAGGATGTATGTGCCAAGATCCACCCATCCTACTGTGGGCCTG CCATCCTCAGGTTCAGGCAGCTGCTGGCTGAACGAGAGCCCGAGGTGCAGGAGGTGGCGCGGCTGTTCCG CTCCGTGTTGCAGGAGGCCCTGGAGAAGATGAAGCAGGAGGAGGAGGCCCATAAGCTGACACGTCAGTGG AGCTTGAGGCCCCGCGGCAGCCTGTCGTCCTTCAAGACCCGCGCTCGCATCGCACCCTTTGCCAGTGACA TCCGAACCATCTCGGAGGATGTGGAGCGCGATGCGCCACCCCCGCCGCGCACCTGGAGCATGCCGGAGTT CAGGGCGCCCCAGGCCGACTGACCACCCAGGGCAATCACCCTCAGGAGGAATAACGGGCTGGTGACTCTG TCACTA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 65715. Forward Primer - name:065715_F_cDNA_3322402L07Rik, sequence:AAGCAGGACCCCTACAGTGATA; Reverse Primer - name:065715_N_SP6_cDNA_3322402L07Rik, sequence:TAGTGACAGAGTCACCAGCCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14893 same embryo
	EMAGE:14896 same embryo
	EMAGE:14895 same embryo
	EMAGE:14897 same embryo
	EMAGE:14892 same embryo
	EurExpress:euxassay_007914 same experiment
	MGI:4827672 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source