EMAGE mouse gene expression spatial database

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EMAGE:14942

Ccdc114 coiled-coil domain containing 114 ( MGI:2446120)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008023. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008023_01	euxassay_008023_02	euxassay_008023_03	euxassay_008023_04

euxassay_008023_05	euxassay_008023_06	euxassay_008023_07	euxassay_008023_08	euxassay_008023_09

euxassay_008023_10	euxassay_008023_11	euxassay_008023_12	euxassay_008023_13	euxassay_008023_14

euxassay_008023_15	euxassay_008023_16	euxassay_008023_17	euxassay_008023_18	euxassay_008023_19

euxassay_008023_20	euxassay_008023_21	euxassay_008023_22	euxassay_008023_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14942_wholemount_strong.wlz

14942_wholemount_moderate.wlz

14942_wholemount_weak.wlz

14942_wholemount_possible.wlz

14942_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14942_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 08 09

lateral ventricle choroid plexus

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 12 14 15 16 17 moderate expression: see section 13

4th ventricle

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 07 08

pons ventricular layer

strong

regional

strong expression: see section 08 09

midbrain ventricular layer

strong

regional

strong expression: see section 06 07 moderate expression: see section 04 05 08 weak expression: see section 03 09

ventral grey horn

moderate

regional

moderate expression: see section 08 09 10 weak expression: see section 11

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 11 12 18 19 20 weak expression: see section 13 14 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36012
Entity Detected:	Ccdc114, coiled-coil domain containing 114 ( MGI:2446120)
Sequence:	sense strand is shown >T36012 CCGATCTCATCATGTACCAGAAGATGAAAATCCAGAGAAGGATCAGGATCCTTGAAGACCAGCTGGACAG GGTCACCTGTCATTTTGACATCCATCTGGTGAGGAATGCAGCCCTGCGGGAGGAGCTGGAGCTGCTGCGC ATCGAGAGGGGCCGCTACCTGAATATGGACCGCAAGCTGAAGAAGGAGATCCACCTACTCCGGGAGATGG TTGGAGCTCTCAGCACATCCTCTACTTCTGCTTATACTGCCAGGGAAGAGGCCAAGACCAAGATGGGCAT GCTTCAGGAGCGCGCAGAGAAGGAGCTGGCTCAGAGTGATACGGAGGCACAGATCTTGCTGCGACAGATA TCGCATCTGGAGCAGCTGCATCGATTCCTTAAGCTCAAGAACGATGAACGACAACCGGATCCCCGTGTGG TGCAAAAGGCAGAACAGCGAGACTGGGAAGTGTCAGAAGGACTCCGAAAGACCTCCCAAGAGAAGCTAGT GCTGCGTTATGAGGACACTCTGGGCAAACTGGCCCAGCTGACCGGCGAGAGCGACCCTGACCTTTTGGTG GAGAAATACCTGGAGTTGGAGGAACGCAACTTTGCTGAGTTT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 80428. Forward Primer - name:080428_F_cDNA_BC013491, sequence:CCGATCTCATCATGTACCAGAA; Reverse Primer - name:080428_N_SP6_cDNA_BC013491, sequence:AAACTCAGCAAAGTTGCGTTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14945 same embryo
	EMAGE:14943 same embryo
	EMAGE:14944 same embryo
	EMAGE:14941 same embryo
	EMAGE:14940 same embryo
	EurExpress:euxassay_008023 same experiment
	MGI:4823666 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source