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EMAGE:15028

Aff2 AF4/FMR2 family, member 2 ( MGI:1202294)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15028 EMAGE:15028 EMAGE:15028 EMAGE:15028 EMAGE:15028
"Pseudo-wholemount" of euxassay_008103. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008103_01 euxassay_008103_02 euxassay_008103_03 euxassay_008103_04
EMAGE:15028 EMAGE:15028 EMAGE:15028 EMAGE:15028 EMAGE:15028
euxassay_008103_05 euxassay_008103_06 euxassay_008103_07 euxassay_008103_08 euxassay_008103_09
EMAGE:15028 EMAGE:15028 EMAGE:15028 EMAGE:15028 EMAGE:15028
euxassay_008103_10 euxassay_008103_11 euxassay_008103_12 euxassay_008103_13 euxassay_008103_14
EMAGE:15028 EMAGE:15028 EMAGE:15028 EMAGE:15028 EMAGE:15028
euxassay_008103_15 euxassay_008103_16 euxassay_008103_17 euxassay_008103_18 euxassay_008103_19
EMAGE:15028 EMAGE:15028 EMAGE:15028 EMAGE:15028 EMAGE:15028
euxassay_008103_20 euxassay_008103_21 euxassay_008103_22 euxassay_008103_23 euxassay_008103_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15028Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15028_wholemount_strong.wlz
15028_wholemount_moderate.wlz
15028_wholemount_weak.wlz
15028_wholemount_possible.wlz
15028_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15028_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36388
Entity Detected:Aff2, AF4/FMR2 family, member 2 ( MGI:1202294)
Sequence:sense strand is shown

>T36388
GCAGAGAAAATCCCTGAGAAGAAGCAGCGCCTGGAGGACAACACTACTATCTGCCTGCTTCCCCCTTGCA
TCTCACCAGCCCCGCCCCACAAGCCTCCCAGCACTAGAGAAAATTCATCCAGGAGAGCAAACAGGAAAAA
AGAAGAAAAGCTGTTCCCTCCTGCACTTTCCCCACTGGCAGAGGATCCTCCACGTCGCAGAAATGTCAGT
GGCAACAATGGGCATTTCGGTCAGGACAAAAACATTTCCATGGCTGGACAAATCACCTCTAGCAAACCTA
AAAGAAGTGAAGGCAAATTCTGTGCTACTTTCAAGGGGATATCTATAAATGAGGGAGATGCTCCAAAAAA
GGCTGCCTCTGCCACCGCTACTGTCGCCAATATGGCTCTTGCCACTGCTACTGCCACTGCCACTGTTCCT
GCCATTGTCACTGCCACTGTCACAGCTACTGCCACCACCACTGCTACAGCCACTACCACAACCACTACCA
CTACTATTTCTAGCATCACCCCTACAATCACTTCTGGCCTTATGGATAGCAGTCACCTGGAGATGACATC
CTGGGCAGCCCTGCCCCTTCTGTCCAGCAGTAGTGCCAATGTCCGGAGACCCAAGCTCACATTTGATGAC
TCTGTTCACAATGCTGATTTTTACATGCAAGAGGCTAAGAAGCTGAAACACAAAGCTGATGCACTGTTTG
AGAAATTTGGCAAAGCTGTGAATTATGCAGATGCAGCCTTGTCCTTCACTGAATGTGGCAATGCCATGGA
ACGTGATCCTCTGGAAGCGAAGTCCCCATATACCATGTATTCTGAGACTGTGGAGCTCCTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 97734. Forward Primer - name:097734_F_cDNA_Fmr2, sequence:GCAGAGAAAATCCCTGAGAAGA; Reverse Primer - name:097734_N_SP6_cDNA_Fmr2, sequence:GAGGAGCTCCACAGTCTCAGAA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15027 same embryo
 EMAGE:15032 same embryo
 EMAGE:15031 same embryo
 EMAGE:15030 same embryo
 EMAGE:15029 same embryo
 EurExpress:euxassay_008103 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS