EMAGE mouse gene expression spatial database

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EMAGE:15185

Srprb signal recognition particle receptor, B subunit ( MGI:102964)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010365. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010365_01	euxassay_010365_02	euxassay_010365_03	euxassay_010365_04

euxassay_010365_05	euxassay_010365_06	euxassay_010365_07	euxassay_010365_08	euxassay_010365_09

euxassay_010365_10	euxassay_010365_11	euxassay_010365_12	euxassay_010365_13	euxassay_010365_14

euxassay_010365_15	euxassay_010365_16	euxassay_010365_17	euxassay_010365_18	euxassay_010365_19

euxassay_010365_20	euxassay_010365_21	euxassay_010365_22	euxassay_010365_23	euxassay_010365_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15185_wholemount_strong.wlz

15185_wholemount_moderate.wlz

15185_wholemount_weak.wlz

15185_wholemount_possible.wlz

15185_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15185_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 02 03 04 05 06 07 08 17 18 19 20 21 22 moderate expression: see section 16

humerus

strong

regional

strong expression: see section 23 moderate expression: see section 02 03 22 24

tibia

moderate

regional

moderate expression: see section 03 04

femur

strong

regional

strong expression: see section 07 08

naris

moderate

regional

moderate expression: see section 11 12 13 14 15

viscerocranium

moderate

regional

Expression in the turbinate bone.

mandible

strong

regional

strong expression: see section 03 04 05 06 08 09 17 18 19 20 21 22 moderate expression: see section 07 10 11 12 14 15 16

maxilla

strong

regional

strong expression: see section 05 06 08 09 17 18 19 20 21 22 moderate expression: see section 07 11 12 16

basioccipital bone

strong

regional

strong expression: see section 04

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 22 23 24 moderate expression: see section 06 21

clavicle

strong

regional

strong expression: see section 07 08 16 17 18 19 20 moderate expression: see section 06 09 15

sternum

moderate

regional

moderate expression: see section 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30197
Entity Detected:	Srprb, signal recognition particle receptor, B subunit ( MGI:102964)
Sequence:	sense strand is shown >T30197 TTTGTGGTGGACAGTGCGGCGTTCCAGCGGGAGGTGAAAGATGTGGCCGAATTTCTGTACCAGGTCCTCA TCGACAGCATGGCTCTGAAGAACTCACCGTCCTTATTAATAGCCTGCAATAAGCAAGATATCGCAATGGC AAAATCAGCGAAGTTGATCCAGCAGCAGCTTGAGAAGGAGCTCAACACCTTACGAGTCACCCGGTCCGCG GCTCCCAGCACACTGGACAGTTCCAGCACCGCACCTGCTCAGTTGGGGAAGAAAGGCAAAGAATTTGAAT TTTCCCAGTTGCCACTCAAAGTGGAGTTCCTGGAGTGTAGTGCCAAGGGTGGACGAGGGGACACTGGCTC TGCTGACATCCAGGACTTGGAGAAGTGGCTGGCCAAGATTGCCTGAGGAGCATCGCTGGCCACAAGCGTT GGTCATGTGAGACTGACCCTGCAGTTTGAGAAAGGGCGGTGGTGGTGTGGACTGATAAAGAGGGGGCATG TCGTTGAAGCATTTCTTGAGTTCTTGAAACAGTTGTACCACCCCCACCCCCGTCAGCTCCTTTCTCAGCT AGTCCTTCTTTCTTAATTCCCATATTCTTTCTCCCTTTGCTAGGTGTTAGGTGTAGCATCATGTTAGTGT GAGACATAACAGAGAGGGCATCGCTTTGAGAGTAGGGTCGCATCTGAGAAGTCCTTGTCTCCTGCCTCTG CGTTGGTCCCTTTTCCTGGGACCAGATCTGCCTGCTTGTGGAAGAAGTGAACTTGCAGTTGCCCCTGGTG TAGGCATAATACAGTGTCAGTTCTTAGTGGAGTCTGGGGTGTGAAGGTCGACAGTGAAGACAGCTTCAAA GCCACAGGCAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3490766), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 11156. Forward Primer - name:011156_F_IRAV04-07_O05_Srprb, sequence:TTTGTGGTGGACAGTGCG; Reverse Primer - name:011156_R_SP6_IRAV04-07_O05_Srprb, sequence:TGTGCCTGTGGCTTTGAA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15189 same embryo
	EMAGE:15187 same embryo
	EMAGE:15186 same embryo
	EMAGE:15184 same embryo
	EMAGE:15188 same embryo
	EurExpress:euxassay_010365 same experiment
	MGI:4828447 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source