EMAGE mouse gene expression spatial database

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EMAGE:15207

2010106G01Rik RIKEN cDNA 2010106G01 gene ( MGI:1913802)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010397. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010397_01	euxassay_010397_02	euxassay_010397_03	euxassay_010397_04

euxassay_010397_05	euxassay_010397_06	euxassay_010397_07	euxassay_010397_08	euxassay_010397_09

euxassay_010397_10	euxassay_010397_11	euxassay_010397_12	euxassay_010397_13	euxassay_010397_14

euxassay_010397_15	euxassay_010397_16	euxassay_010397_17	euxassay_010397_18	euxassay_010397_19

euxassay_010397_20	euxassay_010397_21	euxassay_010397_22	euxassay_010397_23	euxassay_010397_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15207_wholemount_strong.wlz

15207_wholemount_moderate.wlz

15207_wholemount_weak.wlz

15207_wholemount_possible.wlz

15207_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15207_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 05 weak expression: see section 06 18 19 20 21 22

thymus primordium

moderate

regional

moderate expression: see section 13 14 weak expression: see section 11 12 15

mandible

moderate

regional

moderate expression: see section 05 06 07 08 09 10 17 18 19 20 21 weak expression: see section 04 11 15 16 22

maxilla

moderate

regional

moderate expression: see section 05 06 07 08 09 10 17 18 19 20 21 weak expression: see section 11 15 16 22

orbito-sphenoid

weak

regional

weak expression: see section 01 02 03 22 23 24

clavicle

moderate

regional

moderate expression: see section 05 06 07 08 09 17 weak expression: see section 04 10 16 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30264
Entity Detected:	2010106G01Rik, RIKEN cDNA 2010106G01 gene ( MGI:1913802)
Sequence:	sense strand is shown >T30264 CCCCATCCTGGCCTAACTTTGACTATACTCTGGTGGTTATTTTTGTAATTGCTGTGTTCACTGTGGCCTT AGGAGGATACTGGAGTGGACTTATTGAATTGGAAAACATGAAGTCAGTGGAAGACGCAGAAGACAGAGAG ACAAGAAAAAAGAAGGACGATTACTTAACATTCAGTCCTCTCACGGTTGTTGTGTTCGTGGTCATCTGCT GTATAATGATTGTCTTACTGTATTTCTTCTACAGGTGGCTTGTGTATGTTATGATAGCGATTTTCTGCAT AGCATCGTCAATGAGCCTGTACAACTGTCTTTCTGCATTGATTCATAGGATGCCATGTGGACAGTGCACG ATTTTGTGTTGTGGCAAAAACATTAAAGTGAGCCTGATTTTCCTTTCCGGGCTGTGCATCTCAGTTGCTG TTGTGTGGGCTGTATTTCGAAATGAGGATAGGTGGGCTTGGATTTTACAGGATATCTTAGGAATTGCTTT CTGTCTCCCAAAGGTTTAGTTAGGACTATAGCTGGACAACACTATGCCCAGCAAGTTTTAAATCAAAGTT TGAGTTGAAAATTGGGAACATTTACAGCGATGTTTGCAAATCTGTATATTTTTATGAGTTGGTAAAAATC CCATCATACATAGTGAAAATGCATTTTCTTTTAATTATGTTAAAATTGTGCCTTCACAGTCAGCTTAAAA ACATAGCGTTTGTGTAGTTTCCAAACGAATTATATACATTATATTTTTCTAAAAAAGAAAAAAAAAAGCT ACCCTGACCTTCCTTTGTACTTTTCTTAATGAAATATTTCTCCAAATGCTAAGGTTAGATCATGTGTAAG TGTCAAAGCAAGTTTCTGAGTATATTAAAGATTTATGTAACTTTTAACCCCTCTACAGAAATAAATACTT AAGACTGCAAGAAGACAGTTCATGTATATGGGTGTTTTGCCTGTATGCATCTGTCTGTACACCACATTTG TGCCTGGTACCTGGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5059823), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 29868. Forward Primer - name:029868_F_IRAV71-74_B08_2010106G01Rik, sequence:CCCCATCCTGGCCTAACT; Reverse Primer - name:029868_R_SP6_IRAV71-74_B08_2010106G01Rik, sequence:CACCAGGTACCAGGCACA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15205 same embryo
	EMAGE:15210 same embryo
	EMAGE:15208 same embryo
	EMAGE:15206 same embryo
	EMAGE:15209 same embryo
	EurExpress:euxassay_010397 same experiment
	MGI:4822645 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source