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EMAGE:15241

Fam113a family with sequence similarity 113, member A ( MGI:2442177)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15241 EMAGE:15241 EMAGE:15241 EMAGE:15241 EMAGE:15241
"Pseudo-wholemount" of euxassay_010382. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010382_01 euxassay_010382_02 euxassay_010382_03 euxassay_010382_04
EMAGE:15241 EMAGE:15241 EMAGE:15241 EMAGE:15241 EMAGE:15241
euxassay_010382_05 euxassay_010382_06 euxassay_010382_07 euxassay_010382_08 euxassay_010382_09
EMAGE:15241 EMAGE:15241 EMAGE:15241 EMAGE:15241 EMAGE:15241
euxassay_010382_10 euxassay_010382_11 euxassay_010382_12 euxassay_010382_13 euxassay_010382_14
EMAGE:15241 EMAGE:15241 EMAGE:15241 EMAGE:15241 EMAGE:15241
euxassay_010382_15 euxassay_010382_16 euxassay_010382_17 euxassay_010382_18 euxassay_010382_19
EMAGE:15241 EMAGE:15241 EMAGE:15241 EMAGE:15241
euxassay_010382_20 euxassay_010382_21 euxassay_010382_22 euxassay_010382_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15241Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15241_wholemount_strong.wlz
15241_wholemount_moderate.wlz
15241_wholemount_weak.wlz
15241_wholemount_possible.wlz
15241_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15241_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30511
Entity Detected:Fam113a, family with sequence similarity 113, member A ( MGI:2442177)
Sequence:sense strand is shown

>T30511
ACTTGGCAATGCCTCTGGGGGAACGTGTCACTGGGGGTTTCCTCCTGCCAGAGCTCCAGCCCCTGGCAGT
GTCTCTGCGACAGGATGTGGTTGAGGGGAACTTCTACAGCGCTACACTAGCCGGGAAACACTGTTTCGAC
GTCCTAGATCTCCACTTCCATTTCCGGCATGCTGTCCGTCACCGTCATCGAGATGGTGTCCATTGGGACC
AGCATGCACACCGCCACCTCTCGCACTTACTTCTGGCCCACGTGGCCGATGCCTGGGGTGTGGAGCTGCC
CAAACATGACCGGCTGCCAGACCCATGGATTGAGGACTGGTCAGAGATGGATCATTCATTCCAGGGAAGC
CATAAGCAGCCCCCAGACTTCAGGGAGAAGCTGGCCTTGCCCCTGCTCCCACCTTTCCACTTGCCTCCTC
CCATGTCTTTTCCTTACCCACTTGAGTCCTCACCACCTCCCTTGTTCCCATCCCTGCCCCAGGATACCCC
CTTTTTCCAAGGCCAGCCCTTTCCACCCTATGAATTCAAATACAATGCAATGGAGGACTTCTCAATGCCA
GGATGTGGCCCTGGAATGAACTTTGTGCCTGGCCCCCTGCCACCATCAGTCTCTGGCCCTGTCTCCCACG
GTCAACACCGGGGCCCAGTGGTCCACCGGGGCAAGCCACGTTGTGTTCTGAACAACCCCTACCATGTACC
AAGAATTGGGGGTCCATGCAGGCATCGACTCAGACACTCAGACAGACTGATCCACACATACAAACAGGAC
AGGCGGGGACATGCTCATTCGGGAACATGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:30009464), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 60636. Forward Primer - name:060636_F_IRAV109_f02_A930025D01Rik, sequence:ACTTGGCAATGCCTCTGG; Reverse Primer - name:060636_R_SP6_IRAV109_f02_A930025D01Rik, sequence:GCCATGTTCCCGAATGAG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15242 same embryo
 EMAGE:15243 same embryo
 EMAGE:15244 same embryo
 EMAGE:15245 same embryo
 EMAGE:15246 same embryo
 EurExpress:euxassay_010382 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS