EMAGE mouse gene expression spatial database

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EMAGE:15260

Adam11 a disintegrin and metallopeptidase domain 11 ( MGI:1098667)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010451. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010451_01	euxassay_010451_02	euxassay_010451_03	euxassay_010451_04

euxassay_010451_05	euxassay_010451_06	euxassay_010451_07	euxassay_010451_08	euxassay_010451_09

euxassay_010451_10	euxassay_010451_11	euxassay_010451_12	euxassay_010451_13	euxassay_010451_14

euxassay_010451_15	euxassay_010451_16	euxassay_010451_17	euxassay_010451_18	euxassay_010451_19

euxassay_010451_20	euxassay_010451_21	euxassay_010451_22	euxassay_010451_23	euxassay_010451_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15260_wholemount_strong.wlz

15260_wholemount_moderate.wlz

15260_wholemount_weak.wlz

15260_wholemount_possible.wlz

15260_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15260_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vibrissa

weak

regional

weak expression: see section 08 09 23

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 20 21 22 23

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 18 19

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 08 09 18 19 20

ventral grey horn

weak

regional

weak expression: see section 10 11 12 13 14 15 16

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

neural retina

strong

regional

strong expression: see section 02 03 04 05 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30563
Entity Detected:	Adam11, a disintegrin and metallopeptidase domain 11 ( MGI:1098667)
Sequence:	sense strand is shown >T30563 CCAGTCTGCTCTCCCCAACTGGCCCAAGCTAAGAAGGAAAAGGCAGGTCCGCAGGGGCCACCCCACAGTG CACAGCGAGACCAAGTATGTGGAGTTGATTGTAATCAATGACCACCAGCTGTTTGAGCAGATGCGGCAGT CAGTGGTCCTCACCAGCAACTTTGCAAAATCTGTTGTGAACCTGGCAGATGTGATATACAAGGAACAGCT CAACACAAGAATTGTTCTGGTTGCCATGGAAACGTGGGCAGATGGGGACAAGATCCAGGTGCAGGATGAC CTACTGGAGACCCTGGCCCGGCTTATGGTCTACCGGCGGGAGGGTCTGCCTGAGCCCAGTGATGCCACCC ACCTCTTCTCGGGTAGAACCTTCCAGAGCACCAGCAGCGGGGCGGCCTACGTGGGAGGCATCTGTTCACT GTCCCGGGGTGGAGGTGTGAACGAGTATGGCAACATGGGTGCCATGGCGGTGACCCTGGCCCAGACGCTA GGGCAGAACTTGGGCATGATGTGGAATAAGCACCGGAGCTCAGCAGGGGACTGCAAGTGTCCAGACATTT GGCTGGGCTGCATCATGGAGGACACTGGGTTCTATTTGCCCCGCAAGTTCTCGCGCTGCAGCATCGACGA ATACAACCAGTTTCTGCAGGAGGGAGGCGGGAGCTGCCTGTTCAACAAGCCCCTCAAGCTTCTGGACCCT CCCGAGTGCGGAAACGGCTTCGTGGAGGCGGGAGAGGAGTGCGACTGCGGGTCGGTGCAGGAGTGCAGCC GAGCAGGGGGCAACTGCTGCAAAAAATGCACCTTGACGCACGACGCCATGTGCAGCGATGGGCTCTGTTG TCGCCGCTGCAAGTATGAGCCACGAGGTGTCTCCTGCCGAGAAGCGGTGAATGAGTGTGACATTGCAGAG ACCTGCACCGGCGACTCAAGCCAGTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6400084), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59030. Forward Primer - name:059030_F_IRAV112_a05_Adam11, sequence:CCAGTCTGCTCTCCCCAA; Reverse Primer - name:059030_R_SP6_IRAV112_a05_Adam11, sequence:ACACTGGCTTGAGTCGCC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15262 same embryo
	EMAGE:15258 same embryo
	EMAGE:15259 same embryo
	EMAGE:15263 same embryo
	EMAGE:15261 same embryo
	EurExpress:euxassay_010451 same experiment
	MGI:4822972 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source