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EMAGE:15269

Nfxl1 nuclear transcription factor, X-box binding-like 1 ( MGI:1923646)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15269 EMAGE:15269 EMAGE:15269 EMAGE:15269 EMAGE:15269
"Pseudo-wholemount" of euxassay_010376. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010376_01 euxassay_010376_02 euxassay_010376_03 euxassay_010376_04
EMAGE:15269 EMAGE:15269 EMAGE:15269 EMAGE:15269 EMAGE:15269
euxassay_010376_05 euxassay_010376_06 euxassay_010376_07 euxassay_010376_08 euxassay_010376_09
EMAGE:15269 EMAGE:15269 EMAGE:15269 EMAGE:15269 EMAGE:15269
euxassay_010376_10 euxassay_010376_11 euxassay_010376_12 euxassay_010376_13 euxassay_010376_14
EMAGE:15269 EMAGE:15269 EMAGE:15269 EMAGE:15269 EMAGE:15269
euxassay_010376_15 euxassay_010376_16 euxassay_010376_17 euxassay_010376_18 euxassay_010376_19
EMAGE:15269 EMAGE:15269 EMAGE:15269 EMAGE:15269
euxassay_010376_20 euxassay_010376_21 euxassay_010376_22 euxassay_010376_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15269Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15269_wholemount_strong.wlz
15269_wholemount_moderate.wlz
15269_wholemount_weak.wlz
15269_wholemount_possible.wlz
15269_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15269_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30234
Entity Detected:Nfxl1, nuclear transcription factor, X-box binding-like 1 ( MGI:1923646)
Sequence:sense strand is shown

>T30234
TAAGGCTGGCCCAGAATGCTTTCACTGTGAAGAAGGGTGCTCTAAGTCACGGCCTGCGGGTTGTCCTCAC
CCTTGTGTTTTGCCATGTCACCCTGGAAAATGCCCTCCTTGTGTCCAGATGCTTAGAATAAAATGTCACT
GCAAGATCACAAGCCTGTATGTGGAATGTAGAAAAATAACCACAGCTGATGTAAGCGAAAAGAACCTCCT
CAGTTGCTGCAAAAACCAGTGCCCCAAAGAGCTTCCTTGTGGCCACAGATGCAAAGAGATGTGCCATCCT
GGTGAATGCCCCTTTAATTGCAACCAGAAGGTAAAACTTAGATGCCCTTGTAAAAGAATAAAACGGGAGT
TGCCTTGCAATAAAGTACGTGAAAATCAGGTTTCAGTTGAATGTGATGCAACGTGCAAGGAGATGAAGCG
GAAAGCGTCTGAGGTTAAAGAAGCAGAAGCCAACGCTGCCCTGGAAGAAGAAAAGCGAAGGCAGCAGGCT
GAACTGGAGGCTTTTGAAAACAGACTGAAGGGCCGTCGGAAGAAGAGCAGGAAACGCGATGAAGTGGCCG
TGGAGCTCTCGCCTTGGCAGAAGTATAAGTCCTACCTCCTTCCCGTGTGTGCAGTTGTGGTGGCAGTGCT
TATGTGGTACATCTTCCATGGTGTGGATTAATGATCCTTTAAATCTCCTGACATACCTCAGACTGGAGTT
ATATAAATGTTCAATTTCAGACGTTAGGAAACATGCACTGTAGCATATGACCCACTTCACATTCCTCTCT
GTGCCCTTGTCTGGCTGTAGGTAGGACAGAATGCTTAGTGTCATCTGAGTCAGTACAGTCCCCAGCAGTG
AGACGCTGTCTAGAGTCAGCACGACTGCAGCTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4219165), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 26503. Forward Primer - name:026503_F_IRAV59-62_L01_AW538212, sequence:TAAGGCTGGCCCAGAATG; Reverse Primer - name:026503_R_SP6_IRAV59-62_L01_AW538212, sequence:ACAGCTGCAGTCGTGCTG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15267 same embryo
 EMAGE:15265 same embryo
 EMAGE:15268 same embryo
 EMAGE:15266 same embryo
 EMAGE:15264 same embryo
 EurExpress:euxassay_010376 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS