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EMAGE:15270

Madcam1 mucosal vascular addressin cell adhesion molecule 1 ( MGI:103579)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15270 EMAGE:15270 EMAGE:15270 EMAGE:15270 EMAGE:15270
"Pseudo-wholemount" of euxassay_010430. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010430_01 euxassay_010430_02 euxassay_010430_03 euxassay_010430_04
EMAGE:15270 EMAGE:15270 EMAGE:15270 EMAGE:15270 EMAGE:15270
euxassay_010430_05 euxassay_010430_06 euxassay_010430_07 euxassay_010430_08 euxassay_010430_09
EMAGE:15270 EMAGE:15270 EMAGE:15270 EMAGE:15270 EMAGE:15270
euxassay_010430_10 euxassay_010430_11 euxassay_010430_12 euxassay_010430_13 euxassay_010430_14
EMAGE:15270 EMAGE:15270 EMAGE:15270 EMAGE:15270 EMAGE:15270
euxassay_010430_15 euxassay_010430_16 euxassay_010430_17 euxassay_010430_18 euxassay_010430_19
EMAGE:15270 EMAGE:15270 EMAGE:15270 EMAGE:15270
euxassay_010430_20 euxassay_010430_21 euxassay_010430_22 euxassay_010430_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15270Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15270_wholemount_strong.wlz
15270_wholemount_moderate.wlz
15270_wholemount_weak.wlz
15270_wholemount_possible.wlz
15270_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15270_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
spleen primordium
strong strong
regionalstrong expression: see section 02 03 04 05 06 07
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30275
Entity Detected:Madcam1, mucosal vascular addressin cell adhesion molecule 1 ( MGI:103579)
Sequence:sense strand is shown

>T30275
TAGATCGCGAGAGGCAGGCATGGAATCCATCCTGGCCCTCCTGCTGGCCCTGGCCCTAGTACCCTACCAG
CTCAGCAGAGGACAGTCCTTCCAGGTGAACCCCCCTGAGTCTGAGGTAGCTGTGGCCATGGGCACATCCC
TCCAGATCACCTGCAGCATGTCCTGTGACGAGGGTGTAGCCCGGGTGCACTGGCGTGGTCTGGACACCAG
CTTGGGCAGTGTACAGACCCTCCCAGGCAGCAGTATCCTCTCTGTACGGGGCATGCTGTCAGACACAGGC
ACTCCTGTGTGTGTGGGCTCCTGCGGGAGTCGAAGCTTCCAGCACTCCGTGAAGATCCTTGTGTATGCCT
TCCCAGACCAGCTGGTGGTGTCCCCGGAGTTCCTTGTACCTGGACAGGACCAGGTGGTGTCCTGCACGGC
CCACAACATCTGGCCTGCAGACCCGAACAGTCTCTCCTTTGCCCTGCTACTGGGAGAGCAGAGACTGGAG
GGTGCCCAAGCCCTGGAACCAGAGCAAGAAGAGGAGATACAAGAGGCTGAGGGCACACCACTGTTCCGAA
TGACACAACGCTGGCGGTTACCCTCCCTGGGGACCCCTGCCCCTCCTGCCCTTCACTGCCAGGTCACCAT
GCAGCTGCCCAAACTGGTGCTGACCCATAGAAAGGAGATTCCAGCCTCCACCGTCGTCCTATGGATTGGC
AGCTTGGTGCTGGGGCTGCTTGCACTGGTCTTCCTTGCCTACCGCCTGTGGAAATGCTACCGGCCAGGTC
CTCGCCCAGACACTAGCTCATGTACACACCTATGAAGCTCCATTATGCCAGACTAAAGGAGGCAGAGAGT
GACCAGCTGCAGGATTTGGGGCATCAAGATGATAGTGTGGCCTCTTTCCTTGGTGGTCAGCACATCTATA
AGTTTCTCCTGACTTCTGGGCTTTTCTGCCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4986802), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 28324. Forward Primer - name:028324_F_IRAV71-74_H19_Madcam1, sequence:TAGATCGCGAGAGGCAGG; Reverse Primer - name:028324_R_SP6_IRAV71-74_H19_Madcam1, sequence:CAGGCAGAAAAGCCCAGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15272 same embryo
 EMAGE:15273 same embryo
 EMAGE:15271 same embryo
 EMAGE:15274 same embryo
 EMAGE:15275 same embryo
 EurExpress:euxassay_010430 same experiment
 MGI:4826054 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS