EMAGE mouse gene expression spatial database

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EMAGE:15292

Vav2 vav 2 oncogene ( MGI:102718)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010484. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010484_01	euxassay_010484_02	euxassay_010484_03	euxassay_010484_04

euxassay_010484_05	euxassay_010484_06	euxassay_010484_07	euxassay_010484_08	euxassay_010484_09

euxassay_010484_10	euxassay_010484_11	euxassay_010484_12	euxassay_010484_13	euxassay_010484_14

euxassay_010484_15	euxassay_010484_16	euxassay_010484_17	euxassay_010484_18	euxassay_010484_19

euxassay_010484_20	euxassay_010484_21	euxassay_010484_22	euxassay_010484_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15292_wholemount_strong.wlz

15292_wholemount_moderate.wlz

15292_wholemount_weak.wlz

15292_wholemount_possible.wlz

15292_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15292_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

regional

weak expression: see section 01 02 03 04 16 17 18 19 20

thalamus mantle layer

moderate

regional

moderate expression: see section 12 13 15 weak expression: see section 14

mandible

weak

regional

weak expression: see section 03 04 05 06 07 08 16 17 18 19 20 21

maxilla

weak

regional

weak expression: see section 05 07 08 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30538
Entity Detected:	Vav2, vav 2 oncogene ( MGI:102718)
Sequence:	sense strand is shown >T30538 CAAGTCTCGGGAGCGAACCACCAGCAGGGCCTCAAGCCGGTCTCCAGCTTCCTGCGCTTCCTACAACTTT TCTTTTCTCAGTCCTCAGGGCCTCAGCTTTGCCCCCCAGGCCCCTTCTGCTCCCTTCTGGTCAGTGTTCA CACCGCGGGTCATCGGCACAGCCGTGGCCAGGTACAACTTTGCTGCTCGAGACATGCGGGAGCTGTCACT GCGAGAAGGTGATGTGGTGAAGATCTACAGCCGAATTGGTGGGGACCAGGGCTGGTGGAAAGGAGAGACA AATGGGCGGATCGGTTGGTTTCCTTCGACGTACGTGGAAGAGGAAGGCGTCCAGTGAGGACGAGACCTGG ACAGGACCCGTGGATGTTCTTGGGAGTCTCCCCAGCTCTGAAGTCCACCTCTGGCTTCTCCATAGTTCTG GGAACTGCCTGCTAAGCCTGCAGCTGTTATGGCCCATAGGGATGGGGAGGGTGTTCCACCATCACCTGCC CTAGGTGGCCTGTCCCTGGGTACAGTCCTTGTACATAGAGGAAATCTGTGCTGGCCACATTCAGAGCACC AAGGAGAAGGACCTCAGGCAGTGTGGCTAAGCATGTGTGGTTGACAGCAAAGGTCAGAAGCCACTGCCTG CATCTCTGGTTTAGATGATTGTTCAGAAACCTAGAACTCAACTCATGGTGGCTAGGGAGGGCTAGGGACA GCAGGGGGTTCCTTCCCAGGCCCATCTGCTGCCCCACACTGATCTGCCTGTCTTTTCTGCTGCTCTCTAG AGTATGTGTCAGGTCTTGGGAGGCAGGCAGGACCAGAGCCGAGCTGTGCTGTAGCACACACTAGGCTCAG CCGGGAGCCAGAGCTTGCAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5704852), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59059. Forward Primer - name:059059_F_IRAV111_a03_Vav2, sequence:CAAGTCTCGGGAGCGAAC; Reverse Primer - name:059059_R_SP6_IRAV111_a03_Vav2, sequence:TGTGCAAGCTCTGGCTCC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15291 same embryo
	EMAGE:15288 same embryo
	EMAGE:15290 same embryo
	EMAGE:15287 same embryo
	EMAGE:15289 same embryo
	EurExpress:euxassay_010484 same experiment
	MGI:4829152 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source