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EMAGE:15293

Pycrl pyrroline-5-carboxylate reductase-like ( MGI:1913444)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15293 EMAGE:15293 EMAGE:15293 EMAGE:15293 EMAGE:15293
"Pseudo-wholemount" of euxassay_010492. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010492_01 euxassay_010492_02 euxassay_010492_03 euxassay_010492_04
EMAGE:15293 EMAGE:15293 EMAGE:15293 EMAGE:15293 EMAGE:15293
euxassay_010492_05 euxassay_010492_06 euxassay_010492_07 euxassay_010492_08 euxassay_010492_09
EMAGE:15293 EMAGE:15293 EMAGE:15293 EMAGE:15293 EMAGE:15293
euxassay_010492_10 euxassay_010492_11 euxassay_010492_12 euxassay_010492_13 euxassay_010492_14
EMAGE:15293 EMAGE:15293 EMAGE:15293 EMAGE:15293 EMAGE:15293
euxassay_010492_15 euxassay_010492_16 euxassay_010492_17 euxassay_010492_18 euxassay_010492_19
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euxassay_010492_20 euxassay_010492_21 euxassay_010492_22 euxassay_010492_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15293Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15293_wholemount_strong.wlz
15293_wholemount_moderate.wlz
15293_wholemount_weak.wlz
15293_wholemount_possible.wlz
15293_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15293_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30287
Entity Detected:Pycrl, pyrroline-5-carboxylate reductase-like ( MGI:1913444)
Sequence:sense strand is shown

>T30287
GCTGCAAAACTGCCCACTTGTCATCTTTGCCACCAAACCCCAAGTCCTGCCAACTGTCCTGGCGGAAGTG
GCCCCCATAGTCACCACTGAGCACATCATCGTATCTGTGGCTGCTGGGATCTCTCTGAGCACAATGGAGG
GGCTGTTACCCCCGAACACACGAGTATTGCGAGTCTCTCCCAATCTACCCTGCGTTGTCCAGGAGGGGGC
CATGGTGATGGCCCGGGGCCACCATGCTGGGAACGATGACGCAGAGCTCCTACAGAACTTGCTGGAAGCC
TGTGGGCAGTGCATAGAGGTTCCCGAGTCCTACGTAGATATCCACACCGGTCTCAGTGGCAGTGGTGTGG
CCTTTGTGTGTACATTTTCAGAGGCCCTGGCTGAAGGTGCCATCAAAATGGGCATGCCCAGTGGCCTGGC
CCACCGCATTGCTGCTCAGACCCTGCTGGGGACAGCCAAGATGCTGCAGCAGGAAGGGAAGCACCCAGCC
CAGCTTCGGACAGATGTGCTCACACCAGCTGGAACCACCATCCATGGGTTGCATGCCCTAGAGCGGGGCG
GTTTTCGAGCGGCTACCATGAGTGCGGTGGAAGCAGCTACCTGCCGGGCTAAGGAGCTCAGCAAGAAGTA
AGGCAGGCCTCAGATGAGACTACGGGCTCCTTGCCCAGCTGCAGCCTCTGTGGTGAGAATAGCCCTGGAC
GGGAGATGTAGTGGGCAGTCCTCTAAGTGGAATGGCTAATTTATCCAAGAAGCGGTGACTACTTGTAAGA
TGCTATCAAGACGGGGTTGCCTTGACTGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4987954), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 27760. Forward Primer - name:027760_F_IRAV71-74_P13_Pycrl, sequence:GCTGCAAAACTGCCCACT; Reverse Primer - name:027760_R_SP6_IRAV71-74_P13_Pycrl, sequence:CACAGTCAAGGCAACCCC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15295 same embryo
 EMAGE:15294 same embryo
 EMAGE:15297 same embryo
 EMAGE:15296 same embryo
 EMAGE:15298 same embryo
 EurExpress:euxassay_010492 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS