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EMAGE:15388

Cd68 CD68 antigen ( MGI:88342)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15388 EMAGE:15388 EMAGE:15388 EMAGE:15388 EMAGE:15388
"Pseudo-wholemount" of euxassay_010548. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010548_01 euxassay_010548_02 euxassay_010548_03 euxassay_010548_04
EMAGE:15388 EMAGE:15388 EMAGE:15388 EMAGE:15388 EMAGE:15388
euxassay_010548_05 euxassay_010548_06 euxassay_010548_07 euxassay_010548_08 euxassay_010548_09
EMAGE:15388 EMAGE:15388 EMAGE:15388 EMAGE:15388 EMAGE:15388
euxassay_010548_10 euxassay_010548_11 euxassay_010548_12 euxassay_010548_13 euxassay_010548_14
EMAGE:15388 EMAGE:15388 EMAGE:15388 EMAGE:15388 EMAGE:15388
euxassay_010548_15 euxassay_010548_16 euxassay_010548_17 euxassay_010548_18 euxassay_010548_19
EMAGE:15388 EMAGE:15388 EMAGE:15388
euxassay_010548_20 euxassay_010548_21 euxassay_010548_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15388Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15388_wholemount_strong.wlz
15388_wholemount_moderate.wlz
15388_wholemount_weak.wlz
15388_wholemount_possible.wlz
15388_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15388_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
mesenchyme
strong strong
spottedstrong expression: see section 11 moderate expression: see section 01 02 03 04 05 06 07 08 09 10 12 13 14 15 16 17 18 19 20 21 22
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30826
Entity Detected:Cd68, CD68 antigen ( MGI:88342)
Sequence:sense strand is shown

>T30826
GCCGTTACTCTCCTGCCATCCTTCACGATGACACCTACAGCCACAGAAAGCACAGCCAGCCCTACGACCA
GCCACAGGCCCACCACCACCAGTCACGGAAATGTCACAGTTCACACCAGCTCCGGACCCACAACTGTCAC
TCATAACCCTGCCACCACCACCAGTCATGGGAATGCCACAATTTCTCATGCCACAGTTTCTCCCACCACA
AATGGCACTGCTACTAGTCCAAGATCCTCCACTGTTGGCCCTCACCCTGGACCACCTCCACCCTCGCCTA
GTCCAAGGTCCAAGGGGGCTCTTGGGAACTACACGTGGGCCAACGGCTCCCAGCCTTGTGTTCAGCTCCA
AGCCCAAATTCAAATCCGAATCCTATACCCAATTCAGGGTGGAAGAAAGGCTTGGGGCATATCTGTTTTG
AATCCCAACAAAACCAAGGTCCAGGGAGGTTGTGACGGTACCCATCCCCACCTGTCTCTCTCATTTCCTT
ATGGACAGCTTACCTTTGGATTCAAACAGGACCTACATCAGAGCCCGAGTACAGTCTACCTGGACTACAT
GGCGGTGGAATACAATGTGTCCTTCCCACAGGCAGCACAGTGGACATTCATGGCGCAGAATTCATCTCTT
CGAGAGCTCCAAGCTCCCTTGGGCCAAAGCTTCTGCTGTGGAAATGCAAGCATAGTTCTTTCTCCAGCTG
TTCACCTTGACCTGCTCTCTCTAAGGCTACAGGCTGCTCAGCTGCCTGACAAGGGACACTTCGGGCCATG
TTTCTCTTGCAACCGTGACCAGTCCCTCTTGCTGCCTCTCATCATTGGCCTGGTCCTCCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3981024), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 16900. Forward Primer - name:016900_F_IRAV20-23_B05_Cd68, sequence:GCCGTTACTCTCCTGCCA; Reverse Primer - name:016900_R_SP6_IRAV20-23_B05_Cd68, sequence:GAGGAGGACCAGGCCAAT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15393 same embryo
 EMAGE:15390 same embryo
 EMAGE:15389 same embryo
 EMAGE:15391 same embryo
 EMAGE:15392 same embryo
 EurExpress:euxassay_010548 same experiment
 MGI:4823740 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS