EMAGE mouse gene expression spatial database

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EMAGE:15419

Kctd10 potassium channel tetramerisation domain containing 10 ( MGI:2141207)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010579. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010579_01	euxassay_010579_02	euxassay_010579_03	euxassay_010579_04

euxassay_010579_05	euxassay_010579_06	euxassay_010579_07	euxassay_010579_08	euxassay_010579_09

euxassay_010579_10	euxassay_010579_11	euxassay_010579_12	euxassay_010579_13	euxassay_010579_14

euxassay_010579_15	euxassay_010579_16	euxassay_010579_17	euxassay_010579_18	euxassay_010579_19

euxassay_010579_20	euxassay_010579_21	euxassay_010579_22	euxassay_010579_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15419_wholemount_strong.wlz

15419_wholemount_moderate.wlz

15419_wholemount_weak.wlz

15419_wholemount_possible.wlz

15419_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15419_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 16 20 22 weak expression: see section 01 02 03 04 11 15 17 18 19 21

midgut

moderate

regional

moderate expression: see section 10 15 16 17 18 19 20 21 22 23 weak expression: see section 09 11 12 13 14

mandible

moderate

regional

moderate expression: see section 04 08 09 10 16 17 18 19 20 21 22 weak expression: see section 05 06 07 11 14 15

maxilla

moderate

regional

moderate expression: see section 08 09 10 17 18 19 20 21 22 weak expression: see section 06 07 11

clavicle

weak

regional

weak expression: see section 07 08 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30777
Entity Detected:	Kctd10, potassium channel tetramerisation domain containing 10 ( MGI:2141207)
Sequence:	sense strand is shown >T30777 ATCCATATCAAGCGCCCAGATGACCGGGCCCACCTCCACCAGTGAGCAGGCCGAGCACCCTGCCTTCTGC CCTCCCTCTGCTCCTGCCCCGCCCCCTCAGACCCTGTGCAGGCTTTGGGGCACCTCCCACTTCCCCTGGA GTCTGAGACACTTTTGTAACAAGCCAGATGATTATTTTGTTATTTCTTGACAAAGTAGATTGCTTCTGTG TTGGCCCGGGTGTGCACCCCTTCTGAACATGCTGTGTCTGGGGTCCCACCTCCCGTGAGAGCCTGGAGCC CAGCCTTCTGGGGCCTTGGAGGAAAACGTATGAATGAGTTTGGCGTGTATGTGAGAACCTTTGTTGCAGT ATTTATTTTTATGGGTGTTGACTACCTATTAGGGCCTCTTAGGTGACACTCCCTCAAGGACTTAGTTTGG CAGTTGGGAGGAACTGCGTAGTTGGCAGCCGGCCACCAAGGCGTCCTTGAGCAGCCTGGCCCTGGTGTGT TCTGAGCAGGCAGTCTTTGCTCTCCTGTGAGTCTGCTCTGTGTGCGGTTTTGTACAGAGCCCCTTGGGCA CAGTGGAAGGGGGACGAAGTGTGCTTCTGGCCTCTCCCTGGTTCTCAGCATGGCAGGCACACTCCAGGCT GGGAGCAGACCGATTCCTGGCAGTTGGCTGCTCTCTCGGCTCAGTCCTTTTGATGTACCCCATCCAGGAA GGGGCAGCCATCGCTGCTTTAAGGGTCTACTCCTCTCTTGCATGGCTTCCTTTTCTCATCAAGGGGGTGC CTTGCCCTTTGGTTAGGAACTGGGGTGACCGCAGACACTGGCACCAGGTGAGGACA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3601917), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 15675. Forward Primer - name:015675_F_IRAV16-19_C10_Kctd10, sequence:ATCCATATCAAGCGCCCA; Reverse Primer - name:015675_R_SP6_IRAV16-19_C10_Kctd10, sequence:GTGTCCTCACCTGGTGCC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15421 same embryo
	EMAGE:15420 same embryo
	EMAGE:15417 same embryo
	EMAGE:15418 same embryo
	EMAGE:15422 same embryo
	EurExpress:euxassay_010579 same experiment
	MGI:4825733 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source