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EMAGE:15441

Metrnl meteorin, glial cell differentiation regulator-like ( MGI:2384806)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15441 EMAGE:15441 EMAGE:15441 EMAGE:15441 EMAGE:15441
"Pseudo-wholemount" of euxassay_010650. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010650_01 euxassay_010650_02 euxassay_010650_03 euxassay_010650_04
EMAGE:15441 EMAGE:15441 EMAGE:15441 EMAGE:15441 EMAGE:15441
euxassay_010650_05 euxassay_010650_06 euxassay_010650_07 euxassay_010650_08 euxassay_010650_09
EMAGE:15441 EMAGE:15441 EMAGE:15441 EMAGE:15441 EMAGE:15441
euxassay_010650_10 euxassay_010650_11 euxassay_010650_12 euxassay_010650_13 euxassay_010650_14
EMAGE:15441 EMAGE:15441 EMAGE:15441 EMAGE:15441 EMAGE:15441
euxassay_010650_15 euxassay_010650_16 euxassay_010650_17 euxassay_010650_18 euxassay_010650_19
EMAGE:15441 EMAGE:15441 EMAGE:15441 EMAGE:15441 EMAGE:15441
euxassay_010650_20 euxassay_010650_21 euxassay_010650_22 euxassay_010650_23 euxassay_010650_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15441Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15441_wholemount_strong.wlz
15441_wholemount_moderate.wlz
15441_wholemount_weak.wlz
15441_wholemount_possible.wlz
15441_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15441_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 17 18 19 20 21 22 23
radius
strong strong
regionalstrong expression: see section 01 02
ulna
strong strong
regionalstrong expression: see section 01
humerus
strong strong
regionalstrong expression: see section 02 03 04 23 24
fibula
moderate moderate
regionalmoderate expression: see section 04
tibia
moderate moderate
regionalmoderate expression: see section 03 04
femur
moderate moderate
regionalmoderate expression: see section 04 05 06 21 22 23
vibrissa
moderate moderate
regionalmoderate expression: see section 07 weak expression: see section 06 08 21 22
medulla oblongata basal plate ventricular layer
strong strong
regionalstrong expression: see section 13 weak expression: see section 12
pons ventricular layer
strong strong
regionalstrong expression: see section 13
midbrain ventricular layer
strong strong
regionalstrong expression: see section 13 weak expression: see section 12
spinal cord floor plate
strong strong
regionalstrong expression: see section 12 13 14
naris
strong strong
regionalstrong expression: see section 12 13 17 18 moderate expression: see section 16
mandible
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 11 12 16 17 18 19 20 21 22 23
maxilla
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 16 17 18 19 20 21 22 23
axial skeleton cervical region
strong strong
regionalstrong expression: see section 09 16 moderate expression: see section 08
axial skeleton thoracic region
strong strong
regionalstrong expression: see section 16 moderate expression: see section 08
orbito-sphenoid
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 22 23 24
clavicle
strong strong
regionalstrong expression: see section 20 moderate expression: see section 07 08 09 18 19 weak expression: see section 10 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31191
Entity Detected:Metrnl, meteorin, glial cell differentiation regulator-like ( MGI:2384806)
Sequence:sense strand is shown

>T31191
ACCTACGGCCCAACACCTTCTCACCTGCCCAGAACTTGACTGTGTGCATCAAGCCTTTCAGGGACTCCTC
TGGAGCCAATATTTATTTGGAAAAAACTGGAGAACTAAGACTGTTGGTGCGGGACATCAGAGGTGAGCCT
GGCCAAGTGCAGTGCTTCAGCCTGGAGCAGGGAGGCTTATTTGTGGAGGCGACACCCCAACAGGACATCA
GCAGAAGGACCACAGGCTTCCAGTATGAGCTGATGAGTGGGCAGAGGGGACTGGACCTGCACGTGCTGTC
TGCCCCCTGTCGGCCTTGCAGTGACACTGAGGTCCTCCTTGCCATCTGTACCAGTGACTTTGTTGTCCGA
GGCTTCATTGAGGACGTCACACATGTACCAGAACAGCAAGTGTCAGTCATCTACCTGCGGGTGAACAGGC
TTCACAGGCAGAAGAGCAGGGTCTTCCAGCCAGCTCCTGAGGACAGTGGCCACTGGCTGGGCCATGTCAC
AACACTGCTGCAGTGTGGAGTACGACCAGGGCATGGGGAATTCCTCTTCACTGGACATGTGCACTTTGGG
GAGGCACAACTTGGATGTGCCCCACGCTTTAGTGACTTTCAAAGGATGTACAGGAAAGCAGAAGAAATGG
GCATAAACCCCTGTGAAATCAATATGGAGTGACTTGCAGGGTGACACAGTACTGTTGTCCTTCAGATGAG
CCATGTTTTGTGGGCTCAGTCGCTCTATCATATCCTGATAGAGATTGCAGACTGGTGGCATGGGCCCAGC
CTGGTGCTAGAACTGGGAAGGTACATGCTGCTCTGACCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3672224), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 55073. Forward Primer - name:055073_F_IRAV47_c04_BC019776, sequence:ACCTACGGCCCAACACCT; Reverse Primer - name:055073_R_SP6_IRAV47_c04_BC019776, sequence:GGGGTCAGAGCAGCATGT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15445 same embryo
 EMAGE:15443 same embryo
 EMAGE:15444 same embryo
 EMAGE:15442 same embryo
 EMAGE:15440 same embryo
 EurExpress:euxassay_010650 same experiment
 MGI:4826167 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS