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EMAGE:15504

Dpf2 D4, zinc and double PHD fingers family 2 ( MGI:109529)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15504 EMAGE:15504 EMAGE:15504 EMAGE:15504 EMAGE:15504
"Pseudo-wholemount" of euxassay_010678. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010678_01 euxassay_010678_02 euxassay_010678_03 euxassay_010678_04
EMAGE:15504 EMAGE:15504 EMAGE:15504 EMAGE:15504 EMAGE:15504
euxassay_010678_05 euxassay_010678_06 euxassay_010678_07 euxassay_010678_08 euxassay_010678_09
EMAGE:15504 EMAGE:15504 EMAGE:15504 EMAGE:15504 EMAGE:15504
euxassay_010678_10 euxassay_010678_11 euxassay_010678_12 euxassay_010678_13 euxassay_010678_14
EMAGE:15504 EMAGE:15504 EMAGE:15504 EMAGE:15504 EMAGE:15504
euxassay_010678_15 euxassay_010678_16 euxassay_010678_17 euxassay_010678_18 euxassay_010678_19
EMAGE:15504 EMAGE:15504 EMAGE:15504
euxassay_010678_20 euxassay_010678_21 euxassay_010678_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15504Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15504_wholemount_strong.wlz
15504_wholemount_moderate.wlz
15504_wholemount_weak.wlz
15504_wholemount_possible.wlz
15504_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15504_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30841
Entity Detected:Dpf2, D4, zinc and double PHD fingers family 2 ( MGI:109529)
Sequence:sense strand is shown

>T30841
AGGCCGTTGCTCTCCTCTCTACCTTGGTTTCCATTGCCCCTCTCTCCTCTTTCACTCTGTAGTCCTGCCA
ACCTCCGTTGGCAACAGCACAGGGAGGTGGCAGCTCTGACTGCCTCTAGCCCCGAGCCCTCAGGGAGTAA
GGAGCAGCGTGCTGCTCCAGGGCTGACCTGTGGGTCCAACTTCTCTCTGCTCTCCAAGAAGTGCATTCAC
TCTGCCTGCCTTGGGCCTAAGACCCTGGTGATTACAGGGCTCAAATGGGGTCCTCTGAGAAGGAATATGA
GAGCAGCTCACTTGTCTCAAGCCTTGCCCACCCCTCTTCCCCCAAACCCCCTTTGGTTTCCAGGGTTTTG
CCCCAGAGATGAGCCAGGCTGGGCCTTTCCTGGAAGCAGCTGGAGTGAGCTGGCTGAGTGGCACTTGCCA
GGACCTTTTCATACCCTAGTTCTGCTTCCCTTTGCCTCCTGCCAAAGCAGTCCCCTGTCCTCTGTCATGC
TACATGGGGTTCTGTGCTTGAGCTAGAATGTTCTCGGGCACCTCCTGGCTCTGCCCTGTCCCACAAAGGG
ACGAGCAGCTTCAAACCTGTCCTCCCTGTGCTTGGTGGCTTGCTCACAGGTGCGCTCTGGCTACCCAGAC
ATTTCCTATCCTCAGAACTTCCCATCTTCTGCCCCCATCCTTAGTCCCTTTGCTTTTGTAGGGCGAGGGA
TAGTGTCAGGGGCTGGGCCAGCAGCTTGGGGGCCACAGGGAGAAGTTGGATAATGTGCCTGTTTTTTAAC
TCGATAAAAAAGCCTACCTCCAAAATTCCCTTTTTGTTCTTCCTGAACCTGGGCATTCAGCCTCCTGTCC
TTAACTAAATTAGGAGCCTCTGCCTCCTGCCTGTGTATCCTGGCTCCCAGGACACAGGATGGTCCCCTTT
CCTTGCACGCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4208205), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 17255. Forward Primer - name:017255_F_IRAV20-23_L10_Dpf2, sequence:AGGCCGTTGCTCTCCTCT; Reverse Primer - name:017255_R_SP6_IRAV20-23_L10_Dpf2, sequence:TAGCGTGCAAGGAAAGGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15505 same embryo
 EMAGE:15502 same embryo
 EMAGE:15500 same embryo
 EMAGE:15501 same embryo
 EMAGE:15503 same embryo
 EurExpress:euxassay_010678 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS