EMAGE mouse gene expression spatial database

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EMAGE:15513

Mrpl17 mitochondrial ribosomal protein L17 ( MGI:1351608)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010706. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010706_01	euxassay_010706_02	euxassay_010706_03	euxassay_010706_04

euxassay_010706_05	euxassay_010706_06	euxassay_010706_07	euxassay_010706_08	euxassay_010706_09

euxassay_010706_10	euxassay_010706_11	euxassay_010706_12	euxassay_010706_13	euxassay_010706_14

euxassay_010706_15	euxassay_010706_16	euxassay_010706_17	euxassay_010706_18	euxassay_010706_19

euxassay_010706_20	euxassay_010706_21	euxassay_010706_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15513_wholemount_strong.wlz

15513_wholemount_moderate.wlz

15513_wholemount_weak.wlz

15513_wholemount_possible.wlz

15513_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15513_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 14 15 17 18 20 21 22 weak expression: see section 19

thymus primordium

weak

regional

weak expression: see section 10 11 12 13 14

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 15 16 17

viscerocranium

moderate

regional

Expression in the turbinate bone.

mandible

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 weak expression: see section 02

maxilla

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 15 16 17 18 19

liver

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

axial skeleton

moderate

regional

moderate expression: see section 11 12 13

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 06 19 20 21 22

clavicle

moderate

regional

moderate expression: see section 07 08 09 16 17 18

axial skeleton tail region

moderate

regional

moderate expression: see section 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38303
Entity Detected:	Mrpl17, mitochondrial ribosomal protein L17 ( MGI:1351608)
Sequence:	sense strand is shown >T38303 GAGGTGAGAGCATGAGGTTGTCGCTGGCTGCCGCTATCTCCCACGGCCGCGTCTACCGCCGCCTGGGCCT GGGTCCGGAGTCCCGCATCCATCTGCTGCGGAACTTGCTCACGGGCCTAGTTCGCCACGAACGCATCGAG GCGACATGGGCACGCGCGGACGAGATGAGGGGCTACGCGGAGAAGCTCATCGATTATGGAAAGCTGGGCG ACACCAACGAACGAGCCATGCGTATGGCTGACTTCTGGCTCACAGAGAAGGACTTGATCCCAAAGCTGTT TAAAGTGCTAGCGCCTCGGTTCCAAGGTCAGAATGGGAACTACACAAGAATGCTACAGATCCCGAATCGG AAGGAGCAAGATCGGGCCAAGATGGCGGTGATAGAATATAAAGGGAACTACCTCCCTCCCCTGCCTCTGC CTCACAGAGACAGCAACCTTACTCTCCTAAACCAGCTGCTCCTGGGACTGCAGCAGGACCTGCACCATAA CCAGGACGCAAGCCTCCACAGCTCCTGTACAGTTCAAACACCAAAGACTTAACTGGAGCTGGAGAGTGGT GCATAGGTGTGGTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 145466. Forward Primer - name:145466_F_cDNA_Mrpl17, sequence:GAGGTGAGAGCATGAGGTTGTC; Reverse Primer - name:145466_N_SP6_cDNA_Mrpl17, sequence:CACCACACCTATGCACCACTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15517 same embryo
	EMAGE:15514 same embryo
	EMAGE:15515 same embryo
	EMAGE:15512 same embryo
	EMAGE:15516 same embryo
	EurExpress:euxassay_010706 same experiment
	MGI:4826442 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source