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EMAGE:15613

5730409E04Rik RIKEN cDNA 5730409E04Rik gene ( MGI:3609248)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15613 EMAGE:15613 EMAGE:15613 EMAGE:15613 EMAGE:15613
"Pseudo-wholemount" of euxassay_010814. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010814_01 euxassay_010814_02 euxassay_010814_03 euxassay_010814_04
EMAGE:15613 EMAGE:15613 EMAGE:15613 EMAGE:15613 EMAGE:15613
euxassay_010814_05 euxassay_010814_06 euxassay_010814_07 euxassay_010814_08 euxassay_010814_09
EMAGE:15613 EMAGE:15613 EMAGE:15613 EMAGE:15613 EMAGE:15613
euxassay_010814_10 euxassay_010814_11 euxassay_010814_12 euxassay_010814_13 euxassay_010814_14
EMAGE:15613 EMAGE:15613 EMAGE:15613 EMAGE:15613 EMAGE:15613
euxassay_010814_15 euxassay_010814_16 euxassay_010814_17 euxassay_010814_18 euxassay_010814_19
EMAGE:15613
euxassay_010814_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15613Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15613_wholemount_strong.wlz
15613_wholemount_moderate.wlz
15613_wholemount_weak.wlz
15613_wholemount_possible.wlz
15613_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15613_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
facial vii ganglion
weak weak
regionalweak expression: see section 02 16 17 18
glossopharyngeal ix ganglion
weak weak
regionalweak expression: see section 03 04 13 14
trigeminal v ganglion
weak weak
regionalweak expression: see section 01 02 03 04 05 06 15 16 17 18 19
dorsal root ganglion
weak weak
regionalweak expression: see section 02 03 04 05 09 10 11 12
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38361
Entity Detected:5730409E04Rik, RIKEN cDNA 5730409E04Rik gene ( MGI:3609248)
Sequence:sense strand is shown

>T38361
GAGTCACCCTCTGATAACCAGGTCTTCCAAGCCACCAGGCTCCCTGAGGCCGGAGTCCGCAGTCCCCCGG
AGGGTGCGGAGATCCCTGGTGCTGAGCCAGAGAAGTTGAGCGGTGCCAGCAGTGTCTGCTCTCCTCTGGA
GGACATTGGCTATGCCAGCAGTTCCTTGAGCATCGACAGCTTCAGCAGCAGTCCAGAACCTGTCTGCGAT
ACCCCTCGAGGTCCGAGCCCTCTGGATCCCCTTCTGCCCTCCGTGGCCCAGGCCGTGCAGCAGCTGCAGG
CTCAGGAGCGCTACAAAGAACAGGAGAAGGAGAAACACCACGCGCATTTGGTGATGTATCGGCGCTTGGC
GCTGCTCCAGTGGATCCGGGCTCTGCAGCACCAGTTGGTCGACCAGCAGGCCCGACTGCAAGAGAGCTTT
GACACCATCCTGGACAACCGGAAGGAGCTTATCCGCTGTCTGCAACAGAGGGAAGCACCTTGCAGGCACC
AGGACCAGGGCTAAGGGTGTGCACAAGTGTGTGTGCACAAGTGTGTGTGTGCACAAGTGTGTGCATTTGC
AGGCTTTTGAGTGGTTATACACAAGGAAGGATGTGAGTATTAGCTGGCATGTATGCAAGTAAGCATAAGT
GAATATCTATATGCTAACATGTAAGCAGGTATGTAAGGCATGTGTGTTTATGTATGCATGTGTGGCTAGG
TACCCAGGAAT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 107402. Forward Primer - name:107402_F_cDNA_LOC230757, sequence:GAGTCACCCTCTGATAACCAGG; Reverse Primer - name:107402_N_SP6_cDNA_LOC230757, sequence:ATTCCTGGGTACCTAGCCACA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15610 same embryo
 EMAGE:15611 same embryo
 EMAGE:15612 same embryo
 EMAGE:15609 same embryo
 EMAGE:15608 same embryo
 EurExpress:euxassay_010814 same experiment
 MGI:4822764 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS