EMAGE mouse gene expression spatial database

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EMAGE:15652

LOC433482 (LOC433482)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010868. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010868_01	euxassay_010868_02	euxassay_010868_03	euxassay_010868_04

euxassay_010868_05	euxassay_010868_06	euxassay_010868_07	euxassay_010868_08	euxassay_010868_09

euxassay_010868_10	euxassay_010868_11	euxassay_010868_12	euxassay_010868_13	euxassay_010868_14

euxassay_010868_15	euxassay_010868_16	euxassay_010868_17	euxassay_010868_18	euxassay_010868_19

euxassay_010868_20	euxassay_010868_21	euxassay_010868_22	euxassay_010868_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15652_wholemount_strong.wlz

15652_wholemount_moderate.wlz

15652_wholemount_weak.wlz

15652_wholemount_possible.wlz

15652_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15652_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

regional

weak expression: see section 02 03 04 05 06 08 16 17 18 19 20 21

humerus

weak

regional

weak expression: see section 01 20 21 23

fibula

weak

regional

weak expression: see section 01 22

tibia

weak

regional

weak expression: see section 01 21 22

femur

weak

regional

weak expression: see section 02 03 04 05 19 20 21

axial skeleton cervical region

moderate

regional

moderate expression: see section 16

scapula

moderate

regional

moderate expression: see section 20 weak expression: see section 02 03 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38429
Entity Detected:	LOC433482, (LOC433482)
Sequence:	sense strand is shown >T38429 TAAAGGACCGATAGTGGAAGGAGCAATGGGCAGCCAGGACACCAGGACTTGAGGCAGAGTGACATTCTGG AGTCGGATTCAAGGAAGATCACGGGGTGTCCTCGGCTGACCTTTGCAGAGATTGGGTAGGGGGCAAGCCA CCGAGGGTGAGGGCCGGATGATTGGCGGTGCAGGTGCGTGTGCATACGAGCGTACCCAGGAGCTGGGAGG TAGAGAGATCTTTGTTGAGTTCTACCTGCGGCGCCGCCTGCCGTGTGTCAAATGCGCCTGTGCTGGCCAG ATCCCAAGAGTCACAGTGCTGAGTTGCAGAGCACACATTGTGATCCGCCACTTTCTCCTTACAGCGAGGC AGAGGGGTCAGCGAACCCCCGGCAGGAGGGTGCTTGGGCTGTTGCAGGCAGTGATTGAGGGCGGTACAGC TATTAGTGCAGGGGTTCTGGATTCCAGCTGGGAAGGGACAGGGCCATTTTCCCCCTTGAAGGCCTATGGG TTTCCCCCTCTGAGCTGTTACTCCTCTCTTAGCCTGGGCTCCCTGGAGCTTGGCGTCCCACACTCATCCT GCGATGGAGGTTCTTCGACGGCTGGAGCATTGCAGGGTTTGCCGAAGTTCAGCCGGAAAGTGATG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 108172. Forward Primer - name:108172_F_cDNA_LOC433482, sequence:TAAAGGACCGATAGTGGAAGGA; Reverse Primer - name:108172_N_SP6_cDNA_LOC433482, sequence:CATCACTTTCCGGCTGAACTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15651 same embryo
	EMAGE:15654 same embryo
	EMAGE:15653 same embryo
	EMAGE:15650 same embryo
	EMAGE:15655 same embryo
	EurExpress:euxassay_010868 same experiment
	MGI:4823427 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	BrainStars same gene
	ViBrism same gene
Data Source