EMAGE mouse gene expression spatial database

EMAGE:15714

Hcn2 hyperpolarization-activated, cyclic nucleotide-gated K+ 2 ( MGI:1298210)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010859. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010859_01	euxassay_010859_02	euxassay_010859_03	euxassay_010859_04

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euxassay_010859_10	euxassay_010859_11	euxassay_010859_12	euxassay_010859_13	euxassay_010859_14

euxassay_010859_15	euxassay_010859_16	euxassay_010859_17	euxassay_010859_18	euxassay_010859_19

euxassay_010859_20	euxassay_010859_21	euxassay_010859_22

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Expression pattern clarity:
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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

ventral grey horn

moderate

regional

moderate expression: see section 07 weak expression: see section 08 09 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38441
Entity Detected:	Hcn2, hyperpolarization-activated, cyclic nucleotide-gated K+ 2 ( MGI:1298210)
Sequence:	sense strand is shown >T38441 GAGCAATACATGTCCTTCCACAAACTGCCCGCTGACTTCCGCCAGAAGATCCACGATTACTATGAACACC GGTACCAAGGGAAGATGTTTGATGAGGACAGCATCCTTGGGGAACTCAACGGGCCACTGCGTGAGGAGAT TGTGAACTTCAACTGCCGGAAGCTGGTGGCTTCCATGCCGCTGTTTGCCAATGCAGACCCCAACTTCGTC ACAGCCATGCTGACAAAGCTCAAATTTGAGGTCTTCCAGCCTGGAGATTACATCATCCGAGAGGGGACCA TCGGGAAGAAGATGTACTTCATCCAGCATGGGGTGGTGAGCGTGCTCACCAAGGGCAACAAGGAGATGAA GCTGTCGGATGGCTCCTATTTCGGGGAGATCTGCTTGCTCACGAGGGGCCGGCGTACGGCCAGCGTGCGA GCTGACACCTACTGTCGCCTCTACTCACTGAGTGTGGACAATTTCAACGAGGTGCTGGAGGAATACCCCA TGATGCGGCGTGCCTTTGAGACTGTGGCTATTGACCGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 149006. Forward Primer - name:149006_F_cDNA_Hcn2, sequence:GAGCAATACATGTCCTTCCACA; Reverse Primer - name:149006_N_SP6_cDNA_Hcn2, sequence:CCGGTCAATAGCCACAGTCTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15717 same embryo
	EMAGE:15713 same embryo
	EMAGE:15716 same embryo
	EMAGE:15715 same embryo
	EMAGE:15712 same embryo
	EurExpress:euxassay_010859 same experiment
	MGI:4825335 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source