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EMAGE:15729

Mex3a mex3 homolog A (C. elegans) ( MGI:1919890)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15729 EMAGE:15729 EMAGE:15729 EMAGE:15729 EMAGE:15729
"Pseudo-wholemount" of euxassay_010898. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010898_01 euxassay_010898_02 euxassay_010898_03 euxassay_010898_04
EMAGE:15729 EMAGE:15729 EMAGE:15729 EMAGE:15729 EMAGE:15729
euxassay_010898_05 euxassay_010898_06 euxassay_010898_07 euxassay_010898_08 euxassay_010898_09
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euxassay_010898_10 euxassay_010898_11 euxassay_010898_12 euxassay_010898_13 euxassay_010898_14
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euxassay_010898_15 euxassay_010898_16 euxassay_010898_17 euxassay_010898_18 euxassay_010898_19
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euxassay_010898_20 euxassay_010898_21 euxassay_010898_22 euxassay_010898_23 euxassay_010898_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15729Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15729_wholemount_strong.wlz
15729_wholemount_moderate.wlz
15729_wholemount_weak.wlz
15729_wholemount_possible.wlz
15729_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15729_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
neural retina
weak weak
regionalweak expression: see section 01 02 03 04 22 23 24
nasal cavity olfactory epithelium
weak weak
regionalweak expression: see section 09 10 11 12 15 16 17 18 19
vomeronasal organ
weak weak
regionalweak expression: see section 12
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38932
Entity Detected:Mex3a, mex3 homolog A (C. elegans) ( MGI:1919890)
Sequence:sense strand is shown

>T38932
GAGGGGTAAGAACAGGGAGACTTGCCATCTGTAGTTAGAGGTGCTCTTCCTGATTCGCTAGCCCTCTGGT
CCTGACCTCCCATCTCCTAACATGACCCTTTAGCCCACACACACACACACACACACACACACACACACAC
TCACTCACTCACTCATATCCTGAGTTCCCAACAGTATAGGGAGATGGGGGCCTATTAAAGATCTGCATAG
ATCTTCCAGGGTGATGGAGGATGTCGATAAAGGGCTCACAACTTACCCATCTTACAAAGCTGGGCTAGAG
ATCTGGAAAGGGGCTTCTCTTCCCCCTTCAATTGTTTGCTTGGCTTTTCCCCCAATTTTTTTTTGTTTGT
TTGTTTCTGGATCTGCCCTTTGGTTTCTGTTGTTGTTGTTGTTGTTTTCCCTATTCTGTCCCCCTCTCCC
TCAGCCCCCAGCCACATGTTTCTTTCCATCCTCTTACCTCTTCTAGCCCAGCTTTGGGGATATC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 265282. Forward Primer - name:265282_F_cDNA_2700083E18Rik, sequence:GAGGGGTAAGAACAGGGAGACT; Reverse Primer - name:265282_N_SP6_cDNA_2700083E18Rik, sequence:GATATCCCCAAAGCTGGGCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15725 same embryo
 EMAGE:15724 same embryo
 EMAGE:15728 same embryo
 EMAGE:15726 same embryo
 EMAGE:15727 same embryo
 EurExpress:euxassay_010898 same experiment
 MGI:4826173 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS