EMAGE mouse gene expression spatial database

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EMAGE:15747

Egr3 early growth response 3 ( MGI:1306780)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010934. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010934_01	euxassay_010934_02	euxassay_010934_03	euxassay_010934_04

euxassay_010934_05	euxassay_010934_06	euxassay_010934_07	euxassay_010934_08	euxassay_010934_09

euxassay_010934_10	euxassay_010934_11	euxassay_010934_12	euxassay_010934_13	euxassay_010934_14

euxassay_010934_15	euxassay_010934_16	euxassay_010934_17	euxassay_010934_18	euxassay_010934_19

euxassay_010934_20	euxassay_010934_21	euxassay_010934_22	euxassay_010934_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15747_wholemount_strong.wlz

15747_wholemount_moderate.wlz

15747_wholemount_weak.wlz

15747_wholemount_possible.wlz

15747_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15747_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 15 16 17 19 20 21 22 23

lower jaw incisor

moderate

regional

moderate expression: see section 12 13

lower jaw molar

strong

regional

strong expression: see section 07 08 19

upper jaw incisor

moderate

regional

moderate expression: see section 12 13 17

upper jaw molar

strong

regional

strong expression: see section 07 08 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39032
Entity Detected:	Egr3, early growth response 3 ( MGI:1306780)
Sequence:	sense strand is shown >T39032 AATTGCCTGACAATCTGTACCCCGAGGAGATCCCCAGCGCGCTCAACCTCTTCTCCGGCAGCAGCGACTC GGTAGCCCATTACAATCAGATGGCTACAGAGAATGTGATGGACATCGGTCTGACCAACGAGAAGCCCAAT CCGGAACTCTCTTATTCGAGCTCTTTCCAGCCAGCCCCCGGCAACAAGACCGTGACCTACTTGGGAAAGT TCGCTTTCGACTCTCCTTCCAACTGGTGCCAGGACAACATCATTAGCCTCATGAGTGCCGGCATCTTGGG GGTGCCCCCGGCCTCAGGGGCGCTCAGTACGCAGACGTCCACGGCTAGCATGGTGCAGCCTCCTCAGGGA GACGTGGAGGCCATGTATCCGGCGCTGCCTCCTTATTCCAACTGCGGCGATCTCTACTCGGAGCCCGTGT CTTTCCACGACCCCCAGGGAAACCCTGGGCTCGCCTATTCCCCCCAGGATTACCAATCGGCCAAGCCGGC CTTGGACAGCAATCTTTTCCCCATGATTCCTGACTACAACCTGTACCACCATCCCAACGACATGGGCTCC ATTCCGGAACATAAGCCCTTCCAGGGCATGGACCCCATCCGGGTCAACCCACCCCCTATTACCCCACTGG AGACCATCAA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 100205. Forward Primer - name:100205_F_cDNA_Egr3, sequence:AATTGCCTGACAATCTGTACCC; Reverse Primer - name:100205_N_SP6_cDNA_Egr3, sequence:TTGATGGTCTCCAGTGGGGTA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15746 same embryo
	EMAGE:15744 same embryo
	EMAGE:15742 same embryo
	EMAGE:15745 same embryo
	EMAGE:15743 same embryo
	EurExpress:euxassay_010934 same experiment
	MGI:4824495 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source