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EMAGE:15814

S100a3 S100 calcium binding protein A3 ( MGI:1338849)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15814 EMAGE:15814 EMAGE:15814 EMAGE:15814 EMAGE:15814
"Pseudo-wholemount" of euxassay_013218. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013218_01 euxassay_013218_02 euxassay_013218_03 euxassay_013218_04
EMAGE:15814 EMAGE:15814 EMAGE:15814 EMAGE:15814 EMAGE:15814
euxassay_013218_05 euxassay_013218_06 euxassay_013218_07 euxassay_013218_08 euxassay_013218_09
EMAGE:15814 EMAGE:15814 EMAGE:15814 EMAGE:15814 EMAGE:15814
euxassay_013218_10 euxassay_013218_11 euxassay_013218_12 euxassay_013218_13 euxassay_013218_14
EMAGE:15814 EMAGE:15814 EMAGE:15814 EMAGE:15814 EMAGE:15814
euxassay_013218_15 euxassay_013218_16 euxassay_013218_17 euxassay_013218_18 euxassay_013218_19
EMAGE:15814 EMAGE:15814 EMAGE:15814 EMAGE:15814
euxassay_013218_20 euxassay_013218_21 euxassay_013218_22 euxassay_013218_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15814Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15814_wholemount_strong.wlz
15814_wholemount_moderate.wlz
15814_wholemount_weak.wlz
15814_wholemount_possible.wlz
15814_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15814_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
vibrissa
moderate moderate
regionalmoderate expression: see section 05 06 07 20 21
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39062
Entity Detected:S100a3, S100 calcium binding protein A3 ( MGI:1338849)
Sequence:sense strand is shown

>T39062
AAATAGGGACACCCAGTTGGTAGGCTCCTACAGCTGAAGCAACAGCAGCAGTGTGAGGATGACCCGGCCC
CTGGAGCAGGCAGTAGCTGCCATCGTGTGCACCTTCCAGGAGTATGCAGGGCGCTGTGGGGATAAATACA
AGATCTGCCAGTCGGAGCTCAAGGAGTTGTTGCAGAAGGAGCTGCCCACCTGGACGCCGAGTGAGTTCCG
GGAGTGTGACTACAATAAATTCATGAGTGTTCTGGATACCAACAAAGACTGCGAAGTGGACTTTGGGGAG
TACGTGCGCTCACTTGCCAGCCTCTGTCTCTACTGCCACGAGTACTTCAAAGAGTGCCCCCCTGAGCCTC
CTTGCCCCCAGTAGCCTCTGATCCAAAGGTGTACGCTATCCCAGAAGGGCAGGGTCTGCTCCAGTCCTCC
ATCTTTGTCCTTGAGGTGGTCCTGGGTGTGTAGCCACAC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 88873. Forward Primer - name:088873_F_cDNA_S100a3, sequence:AAATAGGGACACCCAGTTGGTA; Reverse Primer - name:088873_N_SP6_cDNA_S100a3, sequence:GTGTGGCTACACACCCAGGAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15810 same embryo
 EMAGE:15813 same embryo
 EMAGE:15812 same embryo
 EMAGE:15809 same embryo
 EMAGE:15811 same embryo
 EurExpress:euxassay_013218 same experiment
 MGI:4827851 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS