EMAGE mouse gene expression spatial database

EMAGE:15925

Gm6537 predicted gene 6537 ( MGI:3645242)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013381. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013381_01	euxassay_013381_02	euxassay_013381_03	euxassay_013381_04

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euxassay_013381_10	euxassay_013381_11	euxassay_013381_12	euxassay_013381_13	euxassay_013381_14

euxassay_013381_15	euxassay_013381_16	euxassay_013381_17	euxassay_013381_18	euxassay_013381_19

euxassay_013381_20	euxassay_013381_21	euxassay_013381_22

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Expression pattern clarity:
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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cochlea

strong

regional

strong expression: see section 03 04 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T40039
Entity Detected:	Gm6537, predicted gene 6537 ( MGI:3645242)
Sequence:	sense strand is shown >T40039 ACTACACCTGGGTTATAAGGCGCTAGCACTGGCTGGCTGCCCGGCAGAAAGGTCCCGGGTCCGGACTCCC CACCATGCTGCTTCGTGTAACCACCTCTGCAGCGCTGCTGCTGCTGCCTCTGCCCCCGCTGCTGGCATTG CTGCTCGGGGCTGCCTCTTCAGCTCCATTGATGCCCAGACCCTCCAAGGAGGAGCTAACCCGCTGTCTAG CAGAGGTGGTCATGGAGGTGCTGACACTGGGCCAGGCCCAGCGGGGCCCCTGCATGCCGGGGAAGACCTC TGAGCATGTGTGGAAGCAACATCCAGAGGGAGGCCTCCAGAAGCGGGTGGCTGAGAAGGCTAGCGATGAG GAGAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 108387. Forward Primer - name:108387_F_cDNA_LOC434157, sequence:ACTACACCTGGGTTATAAGGCG; Reverse Primer - name:108387_N_SP6_cDNA_LOC434157, sequence:GTCTCCTCATCGCTAGCCTTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15929 same embryo
	EMAGE:15927 same embryo
	EMAGE:15924 same embryo
	EMAGE:15928 same embryo
	EMAGE:15926 same embryo
	EurExpress:euxassay_013381 same experiment
	MGI:4825115 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source