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EMAGE:15976

9430029L20Rik RIKEN cDNA 9430029L20 gene ( MGI:1924575)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15976 EMAGE:15976 EMAGE:15976 EMAGE:15976 EMAGE:15976
"Pseudo-wholemount" of euxassay_013419. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013419_01 euxassay_013419_02 euxassay_013419_03 euxassay_013419_04
EMAGE:15976 EMAGE:15976 EMAGE:15976 EMAGE:15976 EMAGE:15976
euxassay_013419_05 euxassay_013419_06 euxassay_013419_07 euxassay_013419_08 euxassay_013419_09
EMAGE:15976 EMAGE:15976 EMAGE:15976 EMAGE:15976 EMAGE:15976
euxassay_013419_10 euxassay_013419_11 euxassay_013419_12 euxassay_013419_13 euxassay_013419_14
EMAGE:15976 EMAGE:15976 EMAGE:15976 EMAGE:15976 EMAGE:15976
euxassay_013419_15 euxassay_013419_16 euxassay_013419_17 euxassay_013419_18 euxassay_013419_19
EMAGE:15976 EMAGE:15976
euxassay_013419_20 euxassay_013419_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15976Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15976_wholemount_strong.wlz
15976_wholemount_moderate.wlz
15976_wholemount_weak.wlz
15976_wholemount_possible.wlz
15976_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15976_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T40397
Entity Detected:9430029L20Rik, RIKEN cDNA 9430029L20 gene ( MGI:1924575)
Sequence:sense strand is shown

>T40397
CTTATGGTACTGTCCATGGGGTCCCCACACTCGGTACCCAGGGCTTCTCCAGTGTGCAATGGGGGAACAG
AAAGATCCTTCTACCCACATACTAGGGTAGACATTGTAAGTCAGGTCTGCCACTGGAGCAGATGACCAGG
CAGTGTCTTGAGTCACAAGGGACGTAGAACTAGCTACTCCTGGGAGAGGATTCTCCTAAGTCTAGAGTCT
CAGTACTGAGACAGGCAACCTGGCCCACAGGCATTGGGCAAATGAGCACAGGCAGCCTACCCACAGTAAG
TACTCTAGGGAAACAGCCGGACCCGCTCTCAGGCCTGACCTAAGTTTCCTGCCCACCCCCAGGAATGCGG
GGGAGGTGTCCTCTCTGCCATCCTGTTTGTTTCTAAATAAAAGTAGTACGGTTTTAAAGCTGTGTGAGAT
GGGGGAGGGGGGGGAGGGGACACTACGACTGGGCTGGGCTAGGCTAGAGTTCCAGAGCAAGTCCACTAAC
TCTGTATGGGTCAAAGAAACCAATCAAACCTCCCTTCCCTGGTGGACTGGGACCGCTGGCCACCAAGAGG
AAGGGCCTCTCCCTCAGCAGTGCTCAGGCCTGTATCAGTAGTAAGCTGGACTGTGCATCCA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 152827. Forward Primer - name:152827_F_cDNA_TC1414533, sequence:CTTATGGTACTGTCCATGGGGT; Reverse Primer - name:152827_N_SP6_cDNA_TC1414533, sequence:TGGATGCACAGTCCAGCTTAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15978 same embryo
 EMAGE:15975 same embryo
 EMAGE:15977 same embryo
 EurExpress:euxassay_013419 same experiment
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS