Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:15988

A330008L17Rik RIKEN cDNA A330008L17 gene ( MGI:2443215)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15988 EMAGE:15988 EMAGE:15988 EMAGE:15988 EMAGE:15988
"Pseudo-wholemount" of euxassay_013420. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013420_01 euxassay_013420_02 euxassay_013420_03 euxassay_013420_04
EMAGE:15988 EMAGE:15988 EMAGE:15988 EMAGE:15988 EMAGE:15988
euxassay_013420_05 euxassay_013420_06 euxassay_013420_07 euxassay_013420_08 euxassay_013420_09
EMAGE:15988 EMAGE:15988 EMAGE:15988 EMAGE:15988 EMAGE:15988
euxassay_013420_10 euxassay_013420_11 euxassay_013420_12 euxassay_013420_13 euxassay_013420_14
EMAGE:15988 EMAGE:15988 EMAGE:15988 EMAGE:15988 EMAGE:15988
euxassay_013420_15 euxassay_013420_16 euxassay_013420_17 euxassay_013420_18 euxassay_013420_19
EMAGE:15988
euxassay_013420_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15988Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15988_wholemount_strong.wlz
15988_wholemount_moderate.wlz
15988_wholemount_weak.wlz
15988_wholemount_possible.wlz
15988_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15988_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
thymus primordium
weak weak
regionalweak expression: see section 08 09 10 11 12
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T45280
Entity Detected:A330008L17Rik, RIKEN cDNA A330008L17 gene ( MGI:2443215)
Sequence:sense strand is shown

>T45280
CTATGAGCACAGAAGAACTGCGAAGGCATTATTCAAATGACTGGGTTTCCGGATACCTGTTACTTGAGTC
TCCTTTACTGCTATTACTATGCAGCATCGTGTTTGAGCTTACCCTCTGACTGAGCTATCTTCAAGGGCAA
ACGTAGAGTGAGGCAAGCTGCCACGTAATCTACTCCATTAGATTCTTTACTTACTGCATCTCTTTAATTA
TTAAAACGCTGTGGATGGTAGCCGTTGTTTGCATCGTTCACTGCACTACAAAACGGAGGCTCAGAAAATG
TAACTGATCCCTTCCCCGGAATATACAGTTAATGACACAACAATTTAAATCAAAATTGTTTGACAGGAGG
TCTGAAGAAATGACTCAGTTTCCAAATGCACAGAGTTTTTGCAGAGGGCCTGACTTTGGTTCCAAACCCC
CACCTTGGGCCTGTAACTCCAGCTCTT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 187289. Forward Primer - name:187289_F_exon_A330008L17Rik, sequence:CTATGAGCACAGAAGAACTGCG; Reverse Primer - name:187289_N_SP6_exon_A330008L17Rik, sequence:AAGAGCTGGAGTTACAGGCCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15987 same embryo
 EMAGE:15989 same embryo
 EMAGE:15984 same embryo
 EMAGE:15985 same embryo
 EMAGE:15986 same embryo
 EurExpress:euxassay_013420 same experiment
 MGI:4822826 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS