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EMAGE:16093

Kcnj2 potassium inwardly-rectifying channel, subfamily J, member 2 ( MGI:104744)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16093 EMAGE:16093 EMAGE:16093 EMAGE:16093 EMAGE:16093
"Pseudo-wholemount" of euxassay_013544. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013544_01 euxassay_013544_02 euxassay_013544_03 euxassay_013544_04
EMAGE:16093 EMAGE:16093 EMAGE:16093 EMAGE:16093 EMAGE:16093
euxassay_013544_05 euxassay_013544_06 euxassay_013544_07 euxassay_013544_08 euxassay_013544_09
EMAGE:16093 EMAGE:16093 EMAGE:16093 EMAGE:16093 EMAGE:16093
euxassay_013544_10 euxassay_013544_11 euxassay_013544_12 euxassay_013544_13 euxassay_013544_14
EMAGE:16093 EMAGE:16093 EMAGE:16093 EMAGE:16093 EMAGE:16093
euxassay_013544_15 euxassay_013544_16 euxassay_013544_17 euxassay_013544_18 euxassay_013544_19
EMAGE:16093 EMAGE:16093 EMAGE:16093 EMAGE:16093
euxassay_013544_20 euxassay_013544_21 euxassay_013544_22 euxassay_013544_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16093Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16093_wholemount_strong.wlz
16093_wholemount_moderate.wlz
16093_wholemount_weak.wlz
16093_wholemount_possible.wlz
16093_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16093_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 08 09 10 11 12 15 16 17 18 19
mandible
strong strong
regionalstrong expression: see section 06 11 16 17 18 19
maxilla
strong strong
regionalstrong expression: see section 06 07 08 17 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T45110
Entity Detected:Kcnj2, potassium inwardly-rectifying channel, subfamily J, member 2 ( MGI:104744)
Sequence:sense strand is shown

>T45110
ATATGACTGGCTGATTCCGTCTTTGGAATACTTACTTTGCTACACAGCCTGACGTTGGTCAGAGGTCCGA
GACAGTTATACAGACCATGGTACTGGTCGAGAGGTGGGTGAAAGCAAGCAGCCACAAGAGACTAAGGCTA
GCACAAAGGTTTCAAGGAAAGACTAAGCTGGATGACTGATGTAAAGTGCTTTGCAGGCCTCCAAGAGACA
TGATGGCACATATCTGTTGTAGTATAAGTTATGGGGTTTTTAATGTATTGTTTTGTGTTTTTACAAAACT
TGAATATGCAGGCAAGCCTCAGTTTGGGTACATGACTTACCTGGAATGTTTCTCTTTAGGGGAACAAGAG
TGATTTTAATGGCATAACACAGGCAAGACTCTGCCTTAATTTTTTGAAAAGCTGCTAACTACATGAACAC
GAA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 219207. Forward Primer - name:219207_F_exon_Kcnj2, sequence:ATATGACTGGCTGATTCCGTCT; Reverse Primer - name:219207_N_SP6_exon_Kcnj2, sequence:TTCGTGTTCATGTAGTTAGCAGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16091 same embryo
 EMAGE:16092 same embryo
 EMAGE:16094 same embryo
 EMAGE:16097 same embryo
 EMAGE:16096 same embryo
 EMAGE:16095 same embryo
 EurExpress:euxassay_013544 same experiment
 MGI:4825721 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS