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EMAGE:16115

Mob3c MOB kinase activator 3C ( MGI:2140623)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16115 EMAGE:16115 EMAGE:16115 EMAGE:16115 EMAGE:16115
"Pseudo-wholemount" of euxassay_013546. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013546_01 euxassay_013546_02 euxassay_013546_03 euxassay_013546_04
EMAGE:16115 EMAGE:16115 EMAGE:16115 EMAGE:16115 EMAGE:16115
euxassay_013546_05 euxassay_013546_06 euxassay_013546_07 euxassay_013546_08 euxassay_013546_09
EMAGE:16115 EMAGE:16115 EMAGE:16115 EMAGE:16115 EMAGE:16115
euxassay_013546_10 euxassay_013546_11 euxassay_013546_12 euxassay_013546_13 euxassay_013546_14
EMAGE:16115 EMAGE:16115 EMAGE:16115 EMAGE:16115 EMAGE:16115
euxassay_013546_15 euxassay_013546_16 euxassay_013546_17 euxassay_013546_18 euxassay_013546_19
EMAGE:16115 EMAGE:16115 EMAGE:16115
euxassay_013546_20 euxassay_013546_21 euxassay_013546_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16115Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16115_wholemount_strong.wlz
16115_wholemount_moderate.wlz
16115_wholemount_weak.wlz
16115_wholemount_possible.wlz
16115_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16115_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T45000
Entity Detected:Mob3c, MOB kinase activator 3C ( MGI:2140623)
Sequence:sense strand is shown

>T45000
CTGGATCTTGAACTTGGACACAAGATACTTCAATCTCAAACCCAGAATCTACTGACATGACTGAAGGCCA
GCACAAAATAGCCCTCCAGACCAGGGAGCCACTGGAACTTGAATGAATTGAAGAATCCAATTTTCTTCTA
TCTCAGGACACTGAGGATCATGCAACTGATGGAGACCTTCCCATCCTAAAAACACCTGCCTCGGCCCAGC
CTCCTGATACATTCAGGCTTCTTGGATTTCCAAGCCACAGCTATCCAGAGAGGCTAGGATGCTTATTCAT
TAGCCATTACTTAATACCTCCATGCTGAGCCCTCTGCACAGACTCACAGGCAGGGAAACCGGCAGAAATA
GGTTTCTGCCCTTCCAGAGCCTTTATCCTGAGAGGCCAGGAGGGCAAGAAGTCACATAAGAATATTGTCT
CTGTCTCTCAATATGCATTGGAGGAAATTGAAACCGGTAA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 187004. Forward Primer - name:187004_F_exon_Mobkl2c, sequence:CTGGATCTTGAACTTGGACACA; Reverse Primer - name:187004_N_SP6_exon_Mobkl2c, sequence:TTACCGGTTTCAATTTCCTCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16120 same embryo
 EMAGE:16116 same embryo
 EMAGE:16119 same embryo
 EMAGE:16118 same embryo
 EMAGE:16117 same embryo
 EurExpress:euxassay_013546 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS