EMAGE mouse gene expression spatial database

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EMAGE:16124

Gdf11 growth differentiation factor 11 ( MGI:1338027)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013538. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013538_01	euxassay_013538_02	euxassay_013538_03	euxassay_013538_04

euxassay_013538_05	euxassay_013538_06	euxassay_013538_07	euxassay_013538_08	euxassay_013538_09

euxassay_013538_10	euxassay_013538_11	euxassay_013538_12	euxassay_013538_13	euxassay_013538_14

euxassay_013538_15	euxassay_013538_16	euxassay_013538_17	euxassay_013538_18	euxassay_013538_19

euxassay_013538_20	euxassay_013538_21	euxassay_013538_22	euxassay_013538_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16124_wholemount_strong.wlz

16124_wholemount_moderate.wlz

16124_wholemount_weak.wlz

16124_wholemount_possible.wlz

16124_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16124_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 12 13 14 15 16 17 moderate expression: see section 10 11 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T45078
Entity Detected:	Gdf11, growth differentiation factor 11 ( MGI:1338027)
Sequence:	sense strand is shown >T45078 CATGCAAAAGTATCCACACACCCACTTGGTGCAACAGGCCAACCCAAGAGGCTCTGCTGGGCCCTGCTGC ACCCCTACCAAGATGTCCCCAATCAACATGCTCTACTTCAATGACAAGCAGCAGATTATCTACGGCAAGA TCCCTGGCATGGTGGTGGATCGATGTGGCTGCTCCTAAGTTGTGGGCTACAGTGGATGCCTCCCTCAGAC CCTACCCCAAGAACCCCAGCCCTGGTCCCCATTCCTCAAGCCCTAGAGTGCTCCTCCCCCACCTCTTCCC ATGAACATCACACCTTGTTCCCTGACCAAGCAGTGTGCAATACAACAGAGGGAGGCAGGTGGGGATGGAG GGGGGAGGGGTTGGGAGAAGAGAAGGGCAGTCAGGGTGGGATGTATCTGAGATTTGCAGGTGAGAAGGTT TGGCAAGAAGACAGATGTAGGGACAGAGGAAGAAGATGGATGGATAGAGACAAGAGGACAGACAGAACAC TGAGAAAGCAAAGGAACAGAGAGGCAAAAAGATTAGAGACTAAGCAGACAAGTAGAGAGATTGAAATGGG AGTAAGCAGAGAGCCCTGCCCCAAGCCTGTTTTTCCACAGCAAGGTGAGAGGCTTGGTATAGTTTGGGGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 205017. Forward Primer - name:205017_F_exon_Gdf11, sequence:CATGCAAAAGTATCCACACACC; Reverse Primer - name:205017_N_SP6_exon_Gdf11, sequence:CCCCCAAACTATACCAAGCCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16126 same embryo
	EMAGE:16121 same embryo
	EMAGE:16125 same embryo
	EMAGE:16122 same embryo
	EMAGE:16123 same embryo
	EurExpress:euxassay_013538 same experiment
	MGI:4825033 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source