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EMAGE:16177

Scube1 signal peptide, CUB domain, EGF-like 1 ( MGI:1890616)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16177 EMAGE:16177 EMAGE:16177 EMAGE:16177 EMAGE:16177
"Pseudo-wholemount" of euxassay_016991. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_016991_01 euxassay_016991_02 euxassay_016991_03 euxassay_016991_04
EMAGE:16177 EMAGE:16177 EMAGE:16177 EMAGE:16177 EMAGE:16177
euxassay_016991_05 euxassay_016991_06 euxassay_016991_07 euxassay_016991_08 euxassay_016991_09
EMAGE:16177 EMAGE:16177 EMAGE:16177 EMAGE:16177 EMAGE:16177
euxassay_016991_10 euxassay_016991_11 euxassay_016991_12 euxassay_016991_13 euxassay_016991_14
EMAGE:16177 EMAGE:16177 EMAGE:16177 EMAGE:16177 EMAGE:16177
euxassay_016991_15 euxassay_016991_16 euxassay_016991_17 euxassay_016991_18 euxassay_016991_19
EMAGE:16177 EMAGE:16177 EMAGE:16177 EMAGE:16177 EMAGE:16177
euxassay_016991_20 euxassay_016991_21 euxassay_016991_22 euxassay_016991_23 euxassay_016991_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16177Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16177_wholemount_strong.wlz
16177_wholemount_moderate.wlz
16177_wholemount_weak.wlz
16177_wholemount_possible.wlz
16177_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16177_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T45506
Entity Detected:Scube1, signal peptide, CUB domain, EGF-like 1 ( MGI:1890616)
Sequence:sense strand is shown

>T45506
TGGACTAGGTGTTGTGTGGAAGTATTGACAAGGGTGGGGGTGGGGTGGGGGCTTCACAAGCAACATCACT
CCCCTCAACAGAACGCAATGGAAAGAAAGCATCCCCCCACCCCCTCTGGGTTCGAGCGTTCACCGTCTCC
GCACTGTGCCTGCGGGTCACCAGAGACAAGGCCTTCCTCAGGGACGTACAGGCTGTCTGCTGAGCAGCCC
CCACTCTTCTTTATGGCAACAGGAGGAGAAAGCAAGGCCCAGGAGAGGAGGCCTCCAAGGCAGAAGCTAT
TGGGAGCCTCCCTGATGCTCCTCAGCCTCTTAGAGAGGAGCCCTTGTATGGCCAGGTCACACGGCATCCC
AGTGGCCGGGGAGTCACCCCCTCACGCAGAGACCTTGCCTGTGCCTCAGATGGCTTGCTGCCAGCTTGTC
AGATACACATAGAAGCTTATATCTCTCAAATTAATGGATATTAGCACTTGGAGGAATGTAGGGGGATCTC
GGAATGAACTCCTGGGGTTCCTGGTCTCCAGCCCAGCCTGATGGTGTAAATGTACTGTTTCAGAAAACAC
ATGAGGCTCACCGAAGGCTGAAGATGTGTGAGCCGGCAAGCCAGGCCTGGTGCCACAGCTTGATGAGAGG
GCACCACACATCTGAACACAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 179539. Forward Primer - name:179539_F_exon_Scube1, sequence:TGGACTAGGTGTTGTGTGGAAG; Reverse Primer - name:179539_N_SP6_exon_Scube1, sequence:CTGTGTTCAGATGTGTGGTGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16179 same embryo
 EMAGE:16178 same embryo
 EMAGE:16176 same embryo
 EMAGE:16175 same embryo
 EMAGE:16180 same embryo
 EurExpress:euxassay_016991 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS