EMAGE mouse gene expression spatial database

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EMAGE:16188

Gosr1 golgi SNAP receptor complex member 1 ( MGI:1858260)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017043. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017043_01	euxassay_017043_02	euxassay_017043_03	euxassay_017043_04

euxassay_017043_05	euxassay_017043_06	euxassay_017043_07	euxassay_017043_08	euxassay_017043_09

euxassay_017043_10	euxassay_017043_11	euxassay_017043_12	euxassay_017043_13	euxassay_017043_14

euxassay_017043_15	euxassay_017043_16	euxassay_017043_17	euxassay_017043_18	euxassay_017043_19

euxassay_017043_20	euxassay_017043_21	euxassay_017043_22	euxassay_017043_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16188_wholemount_strong.wlz

16188_wholemount_moderate.wlz

16188_wholemount_weak.wlz

16188_wholemount_possible.wlz

16188_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16188_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6142
Entity Detected:	Gosr1, golgi SNAP receptor complex member 1 ( MGI:1858260)
Sequence:	sense strand is shown >T6142 CAAAGATGGCGGCAGGAACCAGCAATTACTGGGAAGATCTCAGGAAACAAGCTCGGCAGCTGGAAAACGA ACTTGACCTGAAACTCGTTTCCTTCAGTAAACTCTGTACGAGCTACAGCCACAGCGGCTCACGGGATGGA GGACGCGATAGGTACAGTTCTGACACAACACCGCTATTAAATGGATCAAGCCAAGACAGAATGTTTGAGA CAATGGCAATTGAAATTGAACAGCTTTTGGCAAGGCTTACAGGAGTAAACGACAAAATGGCAGAGTACAC CCACAGTGCAGGGGTGCCCTCCCTGAATGCAGCCCTGATGCACACGCTGCAGCGACACAGAGACATTCTG CAGGATTATACACACGAATTTCATAAAACCAAAGCAAACTTTACGGCAATACGGGAAAGGGAGAATCTCA TGGGATCAGTACGGAAAGATATTGAGTCATATAAAAGCGGGTCTGGAGTAAACAACAGGAGAACGGAACT GTTTCTGAAAGAGCATGACCACCTTCGAAACTCTGATCGTCTGATAGAAGAAACAATAAGCATTGCTATG GCAACAAAAGAGAATATGACTTCCCAGAGAGGAATGCTCAAGTCAATTCACAGCAAGATGAACACTCTGG CCAACCGCTTTCCTGCCGTGAACAGCCTGATACAAAGGATCAACCTTAGGAAACGGCGTGACTCGCTCAT CCTTGGAGGTGTCATTGGCATCTGCACCATCCTGTTGCTGCTGTATGCGTTCCACTGAAAGCATAGCCCC AGGACTCTCTGCCCACCACCTTCATGGCCTGATCTGGACTGGGGAGCCTACCAAGGAGAAAGACCATCCC GGCCACGGGCGCCACCAGATGGATTCCAAACTGCACCTGTGGCTCTGACCTGGTCGGGCTGAGCTGAAGC CGAAGTTTTTCTGTGCTATCTTTTCTAATACACATTACTCTGTTTTTAATTTTAAAAACAACAAAAGGTT TCCGTTGCTGTGTCTCCCTAGGAGGTAAGCAGGCAGAAGCTGAGAAGCTGGGCTTCAGTGACTGGCGAGG CCAGATGATCAGCGGGCCTCGGAGCTGGACTCCTCGTAGCACCAGACTCTCTACATCCAAAGATGGCTTC ACTTTCAGCCACGAGATTGGATGTGAATAAAAGTAATTTTTGTCCTTTTATTTAACACTTGTGATGAGTA ATCAGCTGTCCTTTGCTGATACTCTGTGATCTGTATTCTATTCTGTGTCCCTAACTCTTAGCAGTCAAGA AAAATTCCATGTCCCACGGGTTCTGTGAGATGTTGGTAGGCCAGCTTCATGTTTGAGGTGGTCTGAAATG GAGAGTGCTGTAGAAAGGGCGTCTCGTCTCTGCGTCAGCTGTGAGCGCTGCGTCAGCTGTACCGCCGCCA ACACATCAGAATGGCAGACCTTGCCATTGCTTTATTATTTTTTCCACTGGGCTAATATTTGCTTGTACAT GATAAAATTGCATTTCCACTGACAGGCAATCAGCACCTGTCATTGCCCCTAGCGGTCCCATATCATGTGA AGCTTAATACTAGGAAGTGAGGGAGGGGACTTGGTTCTCAGTGAGGTCTGCTCATGAGCCACTGCAAGGA GACAGTGAGAGGGTGGCTCTCTGGCCTTCCTGTCTGTCAGTGTGGTGCCGCCTGGAGCAAGGGTAGCACT GTGACCTGATGTCACAGTATTCGTGCCCTGTGCAGATCTATCCCTTCCTCCTCTGTTGGCGCCTTCCTTC CTACTCCTAGGCCGCCGGATAACCACTGAGGGGGCCTGGGACTCCTGCCTGCACCTCTCTATTACTTTGG GCAGTGACAGAATTGTGAATGTTTCTCAAGGTTAGCCACTTCTCAGCATCTGTAAATTGGTAACTTGTCG TACTGAACTAGCGGATAGTGTAAAGACTCCAACATCTGTGCTCTGCTGGTCCTACCCTGGAGTGGCCTCC AGCACATTGTCCAGGGGCAGTGAGTGGATATTAGAGCTCTTCACCATTCTATGGTGGGGGACGGTGCCTC TGGGCCACCTTGATACACTTACTGCGCATGGACAGACTGACACCCAGTCTGGGACTGACTGACAGTTTTG ACATCCTGCTGTTGAAGTGTTAGTACATTCTAGGTATGAAAGGCATTTGATATATTTTTCTTAATTATGG GGTTTATTTTTCTAAATATGGGTTAATCAGCTTGTTGACTGGGCATGCCCTTAAAAGGAATTTAAATTTC CCTCTATAGACCTTAAAGAATCAGGGAAGTTCATTTGTTAAATTCTCCTTGAGAGAACAGAACTTCTCTT CCCTGACACTTCTATTATCTTTGATTTTTTTCAAAGGGCCTTGATTGGCATTTGTGCCTCAGCTAGACTT TATGGGACTTTGGTTGTTATATAGTGGGCATATATGAAATCTGAAGTATCGTAAATAAAGTTATTTATTT AAATATACAGCCAGTCTTTTCTACTGGTTTGATGACATGTTTCATTCACTAAAGCTGATGAGAAAGCGTA GGCTAGGACATGGTCCTGCTCCACTGCCAGCCGCCACCGCACAGCAGCTGGGCCGCCCCAGGACGCTGCA CTCAGATGAACAGATGGAATTATGTTGCTTGTCTTCTTTTTGAGAGTAGATCAGTTCTTTCAAGGAACGC TAGCTGTAGCGGCTGGGGTCCGACCTTCCATTGTGGTGCCGTGGAGGGAAGAGTGCCCCTCCCTTTCCAG TTCTGCCCTGTACAGGACCCCCTTGGAACCTCGGTTTTGTGTCATTCAATATTTTTTTCAAGTTCGTTTC TCCCATTTTGGGCTTACCCCAGTTATCAGGCTTAGGCATGGACAGGGAGGGATGTCTTGTCAGAGCCTGA GGTAAGATTTGTGACCCCAGTGAACTGTTGCTCCCCGGCCACAGTTTTTAACAGAATACCTTTTTTTAAA AATATATATTTTTAGTAACAGTTCAAAACTTCCCACTTCACAAAAAGATATGAAAAACGGGAATAAACAG CAGAAAATTCCAAGTGTATTTGTCTGCCCTGGCTTGAGAGGTCTCACTGTTTAGCTCCGGCTGGCCTTGA GTTCCTGCCTCGGCTTCCTGAGACCCTGGGACTGCTATCTGATGTATACCACCAACCCTGGCTCCAAACA GTACCTTCCTCCAGTTTCCCATGTGTCCCAGTGACTCAGTGAAGATGTGCAGTCCATTGCTACTGAGATC CACAAACCCATACCAAAGGATGCTCTGCCTCCTGCCCTGCTCACCTCTGCTTGTTCCCTTCCCCTTGATA CCAGCATTGACATTTATTGAGAATGTCTTTCTGCTTCTTCCTGGTACTCGGATCAGAAATGGGGACAGGT GAGGAAGAGCCACTCTAGAGGACACTTTTGTACTGGGACCCAGATAGCTGAGGGCCTCCATGCTGGTCGT GGATAAGTGAGTGGGAAGCAGAGTCATTGCTCTGCCGCCCTGTGAGCCCTGTGGAGTTGAGGCACCTTGG CCTTCACCACATCACTGTCCTGTCTTACTGCTGATGACCTCGGCCTGAGGGGGTGGCTTCTAAGAATGGG TCTAGCCTTTCCGACTTCTCTTTATCTTCTGTCTTTAACCAGGTAAGAGTTTTGGGATTCATTGAGCTTT TGAACTTGTTATATTAAGTGGTTGGGATGCATGGCCATACCCAGGTCATCTGTGGCAGATGCGGCAGAGG AAACAAACCTACCCAAATGACGGATTGTGCACCGTCCTTATTTTACCGTAGAGGGCATCCTTTGGCCTGA TCTCCAAAAGGATGCAGCATTTCCCACTGCGGGCCCCAAACTCATTCGTCCCCCTGAGTGCCCATATGTC CCTTTCTCAGAGAGAATGGGGGACGAGAAACAAGGTCTAAACGTGATAATCTGGTGAGAAGCTGGGAAAG TTGAGTCATGTCTTAAAGAATGTGTTTTTTTTTTTTTTTCAATCTGATCTAAAATCTTAGTACCAAAATA TTTGTACCACACCCTAGTAGCTCTCAGTGATGGGAAATCAGTTCTTACCATACTTTATTCCGATGGAAGA CGATTGACGCCTGGTGAAAAATGCCAATACAGATTTTTTTTTAATCTATTCTTTTCCAAATGCACTTTCT AATTTTTTGCTTTTAAAATAGAGACTTGGAACCAATATTATTGTATTTTTACTTAATTTTTGTTCTCATC TAAATGTGCATTTTCTGACTGTATAGAAGAAACTTTGTTATATAATTTAATAAATTAAATATCTAAATAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3495882 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3495882 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16191 same embryo
	EMAGE:16187 same embryo
	EMAGE:16192 same embryo
	EMAGE:16190 same embryo
	EMAGE:16189 same embryo
	EurExpress:euxassay_017043 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source