EMAGE mouse gene expression spatial database

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EMAGE:16196

Zfp790 zinc finger protein 790 ( MGI:1923431)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017048. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017048_01	euxassay_017048_02	euxassay_017048_03	euxassay_017048_04

euxassay_017048_05	euxassay_017048_06	euxassay_017048_07	euxassay_017048_08	euxassay_017048_09

euxassay_017048_10	euxassay_017048_11	euxassay_017048_12	euxassay_017048_13	euxassay_017048_14

euxassay_017048_15	euxassay_017048_16	euxassay_017048_17	euxassay_017048_18	euxassay_017048_19

euxassay_017048_20	euxassay_017048_21	euxassay_017048_22	euxassay_017048_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16196_wholemount_strong.wlz

16196_wholemount_moderate.wlz

16196_wholemount_weak.wlz

16196_wholemount_possible.wlz

16196_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16196_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6167
Entity Detected:	Zfp790, zinc finger protein 790 ( MGI:1923431)
Sequence:	sense strand is shown >T6167 CCCAGCGCTAAGACCAACCCAGCATTGGCAGTCTGACCAAGAGGAAGCCTCGTCCTGTAGGTGCAAGAGT ACGCAATGACAGCCCTGAGCGTGTGTGAGAGAAAGCATAGGGAGGTTAGATTGCATGCGCGGCTGGATCT GGGAGGAGTTGGACGGCAGAGAAACTGCGAGGAAGAAAACGGCCTTTGTTCTTGTCCACTTAAATGGTCT GTGGAAAGCCAGCTGCCTCATGGTTCTCTTTCCTGTGCGCTGTCTTCTCAGAAGCTGGCCTCATCGACAT AGGAATCAAGACGAGGACTGACTGGCCACTTTTAGGAGTTTCCAATCATGGCCCACGTAATGACATTCAG AGATGTGGCCGTAGACTTCTCTCCAGAGGAGTGGGAGTGTCTGAGCCTGGACCAGAAGAGTTTATACCGA GATGTCATGCTGGAGAACTACAGCCACCTTGTCTCAGTGGGGCTTTGCATTTATCAACCGCATGGGTTCC CTGTGCCAGAGAAAGAGCAAGATGCCTGCATGGATTTGGGCAATGAGCCTAGCAGATTTTGCCCAGATAC AGATTCCCGGTGGCAGATGAAGAAAGCCCTACGGACAAACAGCACGTGCGCAACTCGGTTGTCCCACTGG GAGGGAATGGGAATGGAGCCAAAGCACGGGCTTGCAGCCTCGTGTCCTGCAGCCGACTGGGAAGGCAAAG GTCAGGAGCCACCACAAAGTGAGAATCAAGAGGAAACGCTCCAGCAAATGACACTCGCTTGTGACAAACT GCCCCTTCTGAATCTGCTCACAGCTCTTCAGTGTCCAAGACTGAAGCTAGCAGAGAAAACAAAGGAAGTC AAATCGGAAAAATCCTTTAGTTTTGCACCAGAGCCCGCTCAACTTCAGCTCATTCACACAGGTGAGAAAT TTGAGGAAGATAAAAAACGCGGAAAGACCTTCCCTCCTGGCCCTAAGCTTACTCGCTACCGAGCAGTTCA GGATGGCAAGAAGGCATTTCAGTGTGACGAGTGTGGCAAGGCTTTCAGCTTCCGTTCTAGCCTCACTGGT CACAAGAGAATTCATACTGGGGAGAAGCCGTTCACGTGTAAGGAGTGTGGGAAGGCCTTCAGATTCCACT CACTGCTCAGTGTCCACGTTCGGACTCACACTGGCGAGAAGTCTTACGAGTGTAAGGCATGTGGAAAGTT CTTTAACTACAGCTCAGACCTGACTCGACATCACCGGATCCACACGGGAGAAAAGCCCTATCAGTGTAGG GAATGTGGGAAAGCCTTCAGCTGTGGCTCAGACCTGACTCGGCACGAGAGGATCCACACTGGCGAGAAGC CCTACGAGTGTAATGAGTGTGGAAAGGCCTTTATCCAGCAGTCACATCTCATTAAACATCAGAGAATCCA CACAGGTGAAAAACCTTACGTATGCAAGGAGTGTGGGAAAGCCTTCACGTGCGGGTCACAATTAAGTCAA CATAGGAAGATCCATACTGGCGATAAATTGCATAAATGTAAAGAATGTAAAAACTCCTTTAGTTTCGCCC TAGATCATACCCAGAGGCAGTTAATTTACATTGGTGAGAAGTTCTTTGAAGATAAGGAGAACGGGGAGAC CTTCTTTCCAGGCGCAGAGTTTCCGCAGTGTGAGACAGTTCACACCGGTAAGAAGCTGTACGAGTGTAAA GAGTGTGGCAAGGCTTTTAGTCTGCGCTCCATCGTTAGTAGTCTTAAAAAAATTCATAACAGGGAAAAGC TTTTTAAATGTAAGGACTGTGAAAAGGCCTTCCGTTGCCCCTCAGACCTCTCTCGACATCAGAAGATTCA CACGGGCGAGAAGCCCTACAAGTGTAAGGAATGTGGGAAGGGCTTTATTTGCCGATCCGACCTGGGCCGG CATCAGAGAGTTCACACTGGTGTGAAGCCGTACGTGTGTAAGGAGTGTGGGAAAGCTTTCACTCGAGGGG CACACCTGACTCAGCATCAGAAAATTCATAGTAAGAAATCACACGAATTCAATGAAGGAGAGAAGGCCTT TAGTTCTGGTTTAGAAAACACTCCGCACTCCTTGATCCCTACGGGCGAGAAACTCTGGGAACGTAAGGAA TACGGAGAGGCCGTCCTTCATGACCCAGAGCGGACTCAACTCCAGGCTGCTAAGACAACGTATGACTGTA AGGAGAAGGGCAAGGACTTTAGCTGGCATTCACATGATACAGACCTCAAGGCAGTTCATATTCGGGAGAA ACGTTTTCAATGCAAGGAATGCCCAAAGGCCTTTAGATACAGCTCAGAACTGACCCGACATCAGAGACTC CATACTGGAGAAAAGCCGTATAAATGTCAGGAGTGCTGGAAGGCCTTTGCTTCTGCGTCAGACCTGGCCC GGCACCAGAGGATTCACACTGGTGAGCGGCCCTACGAGTGCAATGAGTGTGGGAAAGCCTTTATTCAACA GTCACACCTCATTAAACATCAGAGAGTTCACAGTGCCGAGCAGCCCAAGGATGTGATGAGCGCAGAGAAG CCATCGCTTGGCAGTCCGATGCTATTGAACATGAGAGAGTTCACACTGGTCAACAATCTTATAAATTTAA GGAGTGTTGGAAAGATTCTGCTCATGGCTCAGCTCAATCCGTGTCAGAAGATTTATACTGATAAGAAATT AAGTTAAGGTGGAGGGCTTGGAAAAGCCTATTTCTGGGTCTCAAATCTTACCCAGCACTAGAGTGTCCAC ATTGGTGGGAAATCTACAGCAGATCTGGGGAAGCCTTTAGTTGTAACTCAAGTCTCTGTCCATGTCAGTC CGTGGGGTAGAGAATCCTACAGTGTAAACAATGTGGAGGCTTTCATGGGTTCACATCTTGCTCAAAGGGA GAAAATTCATGAGAGGAAACTGGATATAAGGAATATGGAAAGACCTTTCTTCATGGTTCAAAATTTAATA GACACCTGGAAAATCATGCTGACTAAGAGAACTGAATTCAAGGAATTTGGAAACATTTATTCATGATTCT GCACTTAACTGATAGTAAATTCTCAAAAGGGACCTGAGAGAATGTGGAAATGCCCTTCAGGTTTGCAGCT GTGGGGAACAGGGCTTGGCAAGCAACCTGGCTATCCCAGAGAGATGGAAAAGTTTTCATCTTGGACTTTC CTCTCATGACAAGAAGTTCATGCTGGCACCGAGCCCCACAAATGTATGAAGTGATTTTAGTTACCATGGC AGCTTGGGTCAACACCTGGATATATATACTTTTTCCTGTGGTATGAAACCCTGAGCTGTGTGTGCTGAAC ACACTGTCTTGTTTCTCAGCTGCATCCCCAGCCCTCACGTTTGAGGAAGCCCATGAAGATAAATATGTTG AAATGTTTTTCTAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3497180 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3497180 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16193 same embryo
	EMAGE:16197 same embryo
	EMAGE:16198 same embryo
	EMAGE:16194 same embryo
	EMAGE:16195 same embryo
	EurExpress:euxassay_017048 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source