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EMAGE:16233

Tbcd tubulin-specific chaperone d ( MGI:1919686)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16233 EMAGE:16233 EMAGE:16233 EMAGE:16233 EMAGE:16233
"Pseudo-wholemount" of euxassay_017097. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_017097_01 euxassay_017097_02 euxassay_017097_03 euxassay_017097_04
EMAGE:16233 EMAGE:16233 EMAGE:16233 EMAGE:16233 EMAGE:16233
euxassay_017097_05 euxassay_017097_06 euxassay_017097_07 euxassay_017097_08 euxassay_017097_09
EMAGE:16233 EMAGE:16233 EMAGE:16233 EMAGE:16233 EMAGE:16233
euxassay_017097_10 euxassay_017097_11 euxassay_017097_12 euxassay_017097_13 euxassay_017097_14
EMAGE:16233 EMAGE:16233 EMAGE:16233 EMAGE:16233 EMAGE:16233
euxassay_017097_15 euxassay_017097_16 euxassay_017097_17 euxassay_017097_18 euxassay_017097_19
EMAGE:16233 EMAGE:16233 EMAGE:16233 EMAGE:16233
euxassay_017097_20 euxassay_017097_21 euxassay_017097_22 euxassay_017097_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16233Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16233_wholemount_strong.wlz
16233_wholemount_moderate.wlz
16233_wholemount_weak.wlz
16233_wholemount_possible.wlz
16233_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16233_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T63024
Entity Detected:Tbcd, tubulin-specific chaperone d ( MGI:1919686)
Sequence:sense strand is shown

>T63024
CTCTGAGGTTTATGCTGCATTGCTGGGCTGCATGAGTGATTACACCACAGACAGCAGAGGGGATGTGGGA
GCCTGGGTTCGTGAGGCTGCCATGACCAGCCTCATGGATTTGATGCTTTTGCTGGCACGGACAGAGCCGG
TGCTGATTGAGGCCCACATCTGTGAGCGCGTCATGTGTTGCGTTGCCCAGCAAGCAAGTGAGAAGATTGA
TCGGTTCCGTGCTCATGCTGCCCGGGTGTTCCTGACTCTCTTGCACTTCGACAGCCCTCCCATTCCCCAT
GTTCCCCACCGCCAAGAACTGGAAAGTCTGTTTCCCAGGTCTGATGTGGCCACTGTGAACTGGAACGCAC
CTTCCCAGGCCTTCCCTCTCATCACCCAGCTCTTGGGATTGCCTACCTATAGATACCATGTCCTGCTGGG
GCTGGCTGTGTCTGTGGGTGGCTTGACAGAGTCTACGGTCAGACATTCTACACAGAGCCTCTTTGAGTAC
ATGAAGGGGATTCAGAAGGATGCTCAGGTCCTGCAGAGCTTCAGTGAGACCCTCCTGAAGGTCTTTGAGG
ACAACCTTTTGAATGATAGGGTGTCTGTATCACTGCTGAAAATGCTGGACCAGCTGTTGGCTAATGGGTG
CTTTGACATCTTTACTGCCGAGGAGAACCACCCCTTTTGTGTGAAGTTGCTCACACTTTGTAAGGAAGAA
ATCAAGAAGTCAAAAGACATCCAGAAACTTCGGTCAAGCATCGCAGTGCTCTGTGGGATGGTGCAGTTCA
ATGGTGATGTGAGAAAGAAGATTCTTCTTCAGCTGTTCCTCCTCCTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 66110. Forward Primer - name:066110_F_cDNA_Tbcd, sequence:CTCTGAGGTTTATGCTGCATTG; Reverse Primer - name:066110_N_SP6_cDNA_Tbcd, sequence:GAGGAGGAGGAACAGCTGAAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16230 same embryo
 EMAGE:16234 same embryo
 EMAGE:16232 same embryo
 EMAGE:16229 same embryo
 EMAGE:16231 same embryo
 EurExpress:euxassay_017097 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS