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EMAGE:16252

Serpinb2 serine (or cysteine) peptidase inhibitor, clade B, member 2 ( MGI:97609)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16252 EMAGE:16252 EMAGE:16252 EMAGE:16252 EMAGE:16252
"Pseudo-wholemount" of euxassay_017092. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_017092_01 euxassay_017092_02 euxassay_017092_03 euxassay_017092_04
EMAGE:16252 EMAGE:16252 EMAGE:16252 EMAGE:16252 EMAGE:16252
euxassay_017092_05 euxassay_017092_06 euxassay_017092_07 euxassay_017092_08 euxassay_017092_09
EMAGE:16252 EMAGE:16252 EMAGE:16252 EMAGE:16252 EMAGE:16252
euxassay_017092_10 euxassay_017092_11 euxassay_017092_12 euxassay_017092_13 euxassay_017092_14
EMAGE:16252 EMAGE:16252 EMAGE:16252 EMAGE:16252 EMAGE:16252
euxassay_017092_15 euxassay_017092_16 euxassay_017092_17 euxassay_017092_18 euxassay_017092_19
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euxassay_017092_20 euxassay_017092_21 euxassay_017092_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16252Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16252_wholemount_strong.wlz
16252_wholemount_moderate.wlz
16252_wholemount_weak.wlz
16252_wholemount_possible.wlz
16252_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16252_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T63078
Entity Detected:Serpinb2, serine (or cysteine) peptidase inhibitor, clade B, member 2 ( MGI:97609)
Sequence:sense strand is shown

>T63078
GTGCTGAAGAAGCTAGGGAAAAGATTAATTCTTGGGTCAAGACTCAAACCAAAGGTGAAATCCCAAACCT
GCTACCCGAAGGTTCTGTAGATGAAGACACCAAGATGGTGCTGGTGAATGCTGTCTACTTCAAAGGAAAG
TGGAAAACTCCATTTGAGAAGAAACTTAATGGGCTTTATCCTTTCCGTGTGAACTCGCATGAGAGCATAC
CTGTCCAGATGATGTTCCTCCATGCAAAGCTGAACATTGGATACATAAAGGACCTGAAGACTCAGATCCT
AGAACTTCCGCATACTGGAAACATCAGCATGCTCCTGTTGCTTCCCGATGAGATTGAGGACGCATCCACT
GGCTTGGAATTGCTGGAAAGTGAAATAAACTTTGCCAACTTCAACAAGTGGATCAGCAAAGACACACTGG
ATGAAGATGATGTTGTGGTCTACATTCCCAAGTTCAAACTGGCACAAAGCTACGAACTCAAGTCCATTCT
TCAAAGCATGGGCATGGAGGATGCCTTCAACAAGGGCAAGGCCAACTTCTCAGGAATGTCTGAGAGGAAT
GACCTTTTTCTTTCTGAGGTGTTCCATCAAGCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 99329. Forward Primer - name:099329_F_cDNA_Serpinb2, sequence:GTGCTGAAGAAGCTAGGGAAAA; Reverse Primer - name:099329_N_SP6_cDNA_Serpinb2, sequence:GGCTTGATGGAACACCTCAGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16251 same embryo
 EMAGE:16248 same embryo
 EMAGE:16250 same embryo
 EMAGE:16247 same embryo
 EMAGE:16249 same embryo
 EurExpress:euxassay_017092 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS