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EMAGE:16263

Ulk2 Unc-51 like kinase 2 (C. elegans) ( MGI:1352758)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16263 EMAGE:16263 EMAGE:16263 EMAGE:16263 EMAGE:16263
"Pseudo-wholemount" of euxassay_017152. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_017152_01 euxassay_017152_02 euxassay_017152_03 euxassay_017152_04
EMAGE:16263 EMAGE:16263 EMAGE:16263 EMAGE:16263 EMAGE:16263
euxassay_017152_05 euxassay_017152_06 euxassay_017152_07 euxassay_017152_08 euxassay_017152_09
EMAGE:16263 EMAGE:16263 EMAGE:16263 EMAGE:16263 EMAGE:16263
euxassay_017152_10 euxassay_017152_11 euxassay_017152_12 euxassay_017152_13 euxassay_017152_14
EMAGE:16263 EMAGE:16263 EMAGE:16263 EMAGE:16263 EMAGE:16263
euxassay_017152_15 euxassay_017152_16 euxassay_017152_17 euxassay_017152_18 euxassay_017152_19
EMAGE:16263 EMAGE:16263 EMAGE:16263
euxassay_017152_20 euxassay_017152_21 euxassay_017152_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16263Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16263_wholemount_strong.wlz
16263_wholemount_moderate.wlz
16263_wholemount_weak.wlz
16263_wholemount_possible.wlz
16263_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16263_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T63271
Entity Detected:Ulk2, Unc-51 like kinase 2 (C. elegans) ( MGI:1352758)
Sequence:sense strand is shown

>T63271
CAGCTCAGGAGGAGGAATCTTAAGAAGAAACAAAAGGTGACTCTTGGATGAATTGTGAAATCTTCAACTT
GATCTAATGTGGACATGATTTTAATCTTCCAAAAATCTTTCATATTGCACTAATTTATTAAAATAACTGT
GTATTGGATTTTGCAATTTAAAACTAACTGAGGCACAATGGACTTGTTTAAATATTTTACTTGATTATAT
ACATGTCCTTTTCAGAATTCATGTGTAATCTCCACTGAACTTTTAAATGGTTGGAAATTGCGTTCATGTG
AACCTTTGCATTTTTCTCACCTGTTATCTTCACCAACAGATCTTAGTGGAATTTTGAGTTGCTGGTTGTT
TGCATTTTTTGTTGTGCGTGCAGAATGTGCATCGACTCCTGTGATCATAGGTTTACTAAAGGCTAAGTTT
ATTTGGGCAGTATTAGAAAGCCATCATGAATCAAGAAATACTCTTGAGAATTTTAATAGGGCCAAATCAA
AGTGATGAATAATGGCTTGTTAGTGATGTGGAGTTTCTACATGAAATTAGTAAGAAATGAGGTTCGTTTT
CCCTTAGGAAATGGGCAGCTCTCTCCAGACTAATGTGTACTTCTCAGTGTTAACCCTGAGTTCACCATAG
CTAGTCATGGCAGATCAGCACCTCTCCAAGAATGGTTCCTGTGTTGTATATTATTTGGTATCTTTTACTT
ACCTGCTTGAATACTTGAATAAACCATTCACCGGTTTTAATCCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 101659. Forward Primer - name:101659_F_cDNA_Ulk2, sequence:CAGCTCAGGAGGAGGAATCTTA; Reverse Primer - name:101659_N_SP6_cDNA_Ulk2, sequence:AGGATTAAAACCGGTGAATGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16262 same embryo
 EMAGE:16265 same embryo
 EMAGE:16266 same embryo
 EMAGE:16261 same embryo
 EMAGE:16264 same embryo
 EurExpress:euxassay_017152 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS