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EMAGE:16275

Slc5a7 solute carrier family 5 (choline transporter), member 7 ( MGI:1927126)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16275 EMAGE:16275 EMAGE:16275 EMAGE:16275 EMAGE:16275
"Pseudo-wholemount" of euxassay_017136. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_017136_01 euxassay_017136_02 euxassay_017136_03 euxassay_017136_04
EMAGE:16275 EMAGE:16275 EMAGE:16275 EMAGE:16275 EMAGE:16275
euxassay_017136_05 euxassay_017136_06 euxassay_017136_07 euxassay_017136_08 euxassay_017136_09
EMAGE:16275 EMAGE:16275 EMAGE:16275 EMAGE:16275 EMAGE:16275
euxassay_017136_10 euxassay_017136_11 euxassay_017136_12 euxassay_017136_13 euxassay_017136_14
EMAGE:16275 EMAGE:16275 EMAGE:16275 EMAGE:16275 EMAGE:16275
euxassay_017136_15 euxassay_017136_16 euxassay_017136_17 euxassay_017136_18 euxassay_017136_19
EMAGE:16275 EMAGE:16275 EMAGE:16275
euxassay_017136_20 euxassay_017136_21 euxassay_017136_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16275Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16275_wholemount_strong.wlz
16275_wholemount_moderate.wlz
16275_wholemount_weak.wlz
16275_wholemount_possible.wlz
16275_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16275_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T63361
Entity Detected:Slc5a7, solute carrier family 5 (choline transporter), member 7 ( MGI:1927126)
Sequence:sense strand is shown

>T63361
CCTCACCAAGAAACTGGAAAACAAACCCTGCTTATTTTGGACATTTATTAATGGAATAGTTTAGTGTTCA
GGACCTACTCTTGACACTGTTCCACATAAGCCATTTTTAGGGACTGGACAGATCACAACAAGTATGTATA
TTGAATATCAAATGTAATAGAAAGCAAACAGTAAAAACAAGTCATAGAAAGAAGAAGCCATCATTTTTAA
TATCTGTTGTGTACATGGATTTCAAGAGGTCAGTCATTAAATGTAGTTTTGCTTCTCTTTGTCTTGTTCA
GAATTGCACACAAACCTTGTGCCCCCAAACCCTAGAATTCTCTTTATAGGTTACAGTTATTATCTCTCAA
AGTATCAAAAGGTGGAAGTCCTTTGCTTAAAAATGTGACTTAAAAATTCCTAGTATGACTTTTAAAATTT
AAAGTTAAATAATTTTACATATTGTAATATCCTACTTTTCAGTGGTGTCTCAATTCGTAGGCCTCTTTAC
TGTATGTATATTACATAAGAAAGATAATTAATTTACCCTCCAGTCTATCTCAGAATCAACTCACTAACTA
TGGACTATTTGAAAGTATGGAGTCTGAGATGCTCAAATGTGGGGTGAGAAAAGAATCAAAGAAAAAAGAA
AACACAGATTAAAAAATAGAAAACTTTGGGCATGAAAAACTACTAGTGACAAGGATGAAATTTCTGGTAA
ACATGGTATTTCAGACCCACAAGGTCTTTCAACAAAGACAAAAGAGTGAATTTTGCCCAACTCATCATCT
GTGCCCTTCCATCGGATTGTTAATGTGCGTTCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 96519. Forward Primer - name:096519_F_cDNA_Slc5a7, sequence:CCTCACCAAGAAACTGGAAAAC; Reverse Primer - name:096519_N_SP6_cDNA_Slc5a7, sequence:AGAACGCACATTAACAATCCG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16277 same embryo
 EMAGE:16273 same embryo
 EMAGE:16278 same embryo
 EMAGE:16276 same embryo
 EMAGE:16274 same embryo
 EurExpress:euxassay_017136 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS