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EMAGE:16287

Sfpq splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated) ( MGI:1918764)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16287 EMAGE:16287 EMAGE:16287 EMAGE:16287 EMAGE:16287
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euxassay_017148_06 euxassay_017148_07 euxassay_017148_08 euxassay_017148_09 euxassay_017148_10
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euxassay_017148_11 euxassay_017148_12 euxassay_017148_13 euxassay_017148_14 euxassay_017148_15
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euxassay_017148_16 euxassay_017148_17 euxassay_017148_18 euxassay_017148_19 euxassay_017148_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16287Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16287_wholemount_strong.wlz
16287_wholemount_moderate.wlz
16287_wholemount_weak.wlz
16287_wholemount_possible.wlz
16287_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16287_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T63391
Entity Detected:Sfpq, splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated) ( MGI:1918764)
Sequence:sense strand is shown

>T63391
GAATCTAGAGCCTTGGCTGAAATCGCCAAAGCTGAGCTTGATGATACTCCCATGAGAGGTAGACAGCTTC
GGGTTCGATTTGCCACACACGCTGCAGCCCTGTCTGTTCGAAATCTCTCTCCTTATGTTTCCAACGAACT
TTTGGAAGAGGCCTTTAGCCAGTTTGGTCCTATTGAAAGGGCTGTTGTAATTGTGGATGATCGCGGAAGA
TCTACAGGGAAAGGCATTGTTGAGTTTGCTTCCAAGCCAGCAGCAAGAAAAGCATTTGAAAGATGCAGTG
AAGGTGTTTTCCTACTGACAACGACTCCTCGCCCAGTCATTGTGGAACCACTTGAACAGTTAGATGACGA
AGATGGTCTTCCTGAAAAGCTGGCCCAGAAGAATCCAATGTATCAAAAGGAGAGAGAAACACCACCTCGT
TTTGCTCAGCATGGCACATTTGAGTATGAATATTCTCAACGATGGAAGTCCTTGGATGAAATGGAAAAAC
AGCAAAGGGAACAAGTTGAAAAAAACATGAAGGATGCTAAAGACAAATTGGAAAGTGAAATGGAAGATGC
TTACCATGAACACCAAGCAAATCTTTTGCGCCAAGATCTGATGAGACGCCAGGAAGAATTAAGGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 65475. Forward Primer - name:065475_F_cDNA_Sfpq, sequence:GAATCTAGAGCCTTGGCTGAAA; Reverse Primer - name:065475_N_SP6_cDNA_Sfpq, sequence:GCCTTAATTCTTCCTGGCGTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16285 same embryo
 EMAGE:16284 same embryo
 EMAGE:16289 same embryo
 EMAGE:16286 same embryo
 EMAGE:16288 same embryo
 EurExpress:euxassay_017148 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS