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EMAGE:16308

Rapgef4 Rap guanine nucleotide exchange factor (GEF) 4 ( MGI:1917723)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16308 EMAGE:16308 EMAGE:16308 EMAGE:16308 EMAGE:16308
euxassay_017162_01 euxassay_017162_02 euxassay_017162_03 euxassay_017162_04 euxassay_017162_05
EMAGE:16308 EMAGE:16308 EMAGE:16308 EMAGE:16308 EMAGE:16308
euxassay_017162_06 euxassay_017162_07 euxassay_017162_08 euxassay_017162_09 euxassay_017162_10
EMAGE:16308 EMAGE:16308 EMAGE:16308 EMAGE:16308 EMAGE:16308
euxassay_017162_11 euxassay_017162_12 euxassay_017162_13 euxassay_017162_14 euxassay_017162_15
EMAGE:16308 EMAGE:16308 EMAGE:16308 EMAGE:16308 EMAGE:16308
euxassay_017162_16 euxassay_017162_17 euxassay_017162_18 euxassay_017162_19 euxassay_017162_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16308Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16308_wholemount_strong.wlz
16308_wholemount_moderate.wlz
16308_wholemount_weak.wlz
16308_wholemount_possible.wlz
16308_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16308_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T63518
Entity Detected:Rapgef4, Rap guanine nucleotide exchange factor (GEF) 4 ( MGI:1917723)
Sequence:sense strand is shown

>T63518
AACTGTGTTGTTTAACCAGGGGGAAGAAGGTACCTCCTGGTACATCATTCTGAAAGGATCCGTGAATGTA
GTCATTTATGGCAAGGGTGTGGTCTGCACCCTGCACGAAGGAGATGACTTTGGCAAGTTAGCTCTAGTGA
ACGATGCTCCAAGAGCTGCCTCCATTGTTCTTCGGGAAGATAATTGTCACTTCCTAAGAGTCGACAAGGA
AGACTTCAATCGGATTCTGAGGGACGTTGAGGCGAATACAGTCAGACTTAAAGAACATGACCAAGATGTC
TTGGTACTGGAGAAGGTCCCAGCAGGGAACAGAGCTGCTAATCAAGGAAACTCACAGCCTCAGCAAAAGT
ATACTGTGATGTCAGGAACACCTGAAAAGATTTTAGAGCATTTTCTAGAAACAATACGCCTTGAGCCATC
GTTGAATGAAGCAACAGATTCGGTTTTAAATGACTTTGTTATGATGCACTGTGTTTTTATGCCAAATACC
CAGCTTTGCCCTGCCCTTGTGGCCCATTACCACGCACAGCCTTCTCAAGGTACCGAGCAGGAGAGAATGG
ATTATGCCCTCAACAACAAGAGGCGGGTCATCCGCTTGGTCCTGCAGTGGGCGGCCATGTATGGCGATCT
CCTCCAAGAAGATGATGTGGCCATGGCCTTCCTGGAGGAGTTCTATGTGTCTGTATCAGATGACGCACGG
ATGATGGCTGCCTTCAAGGAGCAGCTGCCAGAGCTGGAGAAGATTGTCAAGCAAATCTCAGAAGACG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 100534. Forward Primer - name:100534_F_cDNA_Rapgef4, sequence:AACTGTGTTGTTTAACCAGGGG; Reverse Primer - name:100534_N_SP6_cDNA_Rapgef4, sequence:CGTCTTCTGAGATTTGCTTGAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16307 same embryo
 EMAGE:16304 same embryo
 EMAGE:16306 same embryo
 EMAGE:16303 same embryo
 EMAGE:16305 same embryo
 EurExpress:euxassay_017162 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS