EMAGE mouse gene expression spatial database

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EMAGE:16309

Mbtps2 membrane-bound transcription factor peptidase, site 2 ( MGI:2444506)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017176. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017176_01	euxassay_017176_02	euxassay_017176_03	euxassay_017176_04

euxassay_017176_05	euxassay_017176_06	euxassay_017176_07	euxassay_017176_08	euxassay_017176_09

euxassay_017176_10	euxassay_017176_11	euxassay_017176_12	euxassay_017176_13	euxassay_017176_14

euxassay_017176_15	euxassay_017176_16	euxassay_017176_17	euxassay_017176_18	euxassay_017176_19

euxassay_017176_20	euxassay_017176_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16309_wholemount_strong.wlz

16309_wholemount_moderate.wlz

16309_wholemount_weak.wlz

16309_wholemount_possible.wlz

16309_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16309_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4123
Entity Detected:	Mbtps2, membrane-bound transcription factor peptidase, site 2 ( MGI:2444506)
Sequence:	sense strand is shown >T4123 CCGGGCGGGCGCACGGCTGACTGGGGCTCGCAGAAGCAGGCCGCCGGGGCCCGCCGTCCATCCGTCGCTA CGATGATTCCGGTGTCGCTGCTGGTGGTGGTGGTGGGTGGTTGGACTGCCGTCTACCTGGCCGACTTGGT GTTGAAGTCATCTGTGTATTTTAAACATTCTTATGAAGACTGGCTGGAAAACAATGGATTGAGCATCTCT CCTTTTCATATAAGATGGCAAACTTCAATTTTTAATCGTGCCTTTTACAGTTGGGGACGGCGGAAAGCAA GGATGCTTTACCAATGGTTCAATTTTGGAATGGTGTTTGGCGTGATTGCCATGTTCAGCTCTTTTTTTCT CCTTGGAAAAACACTGATGCAGACTTTGGCACAAATGATGGCTGACTCTCCCTCTCCTTATTCTTCCTCT TCCTCTTCCTCCTCTTCCTCTTCCTCCTCTTCCTCTTCCTCCTCCTCTCTTCACAATGAACAGGTGTTGC AAGTTGTGGTTCCCGGTATAAATTTACCTGTCAATCAGCTGACTTACTTCTTCGCTGCAGTCCTCATTAG TGGTGTTGTACATGAAATTGGACACGGTATAGCAGCTATTAGGGAACAAGTTCGATTTAATGGCTTTGGA ATTTTTCTCTTCATTATTTATCCTGGGGCATTTGTTGATCTGTTCACCACCCATTTGCAACTTATATCAC CAGTCCAGCAGCTAAGGATATTTTGTGCAGGTATATGGCATAATTTTGTCCTTGCTCTCCTTGGTATTTT AGCTCTTGTTCTCCTCCCAGTAATCCTCTTGCCTTTTTACTACACTGGAGTTGGGGTACTTATCACTGAA GTCGCAGAGGACTCACCTGCCATTGGACCCAGAGGCCTTTTTGTGGGAGACCTTGTCACTCATCTACAGG ATTGTCCCGTTACTAATGTGCAAGATTGGAATGAATGTTTAGACACCATTGCATATGAGCCCCAAATTGG GTATTGTATAAGTGCATCAACGTTACAACAATTAAGCTTCCCAGTTAGAGCATACAAACGACTAGATGGT TCTACTGAATGCTGTAACAATCACAGCCTCACAGATGTGTGCTTTTCCTACAGAAATAATTTCAATAAAC GTTTGCATACGTGTCTACCTGCCCGGAAAGCAGTTGAAGCTACTCAAGTTTGTAGAAGCAATAAAGATTG CAAAAGCGGCGCAAGTTCTAGCTTCTGTATAGTACCGTCCTTGGAAACTCACACCCGACTAATCAAAGTG AAACATCCCCCGCAAATTGATATGCTGTATGTAGGGCACCCACTGCATCTTCACTACACAGTGAGCATTA CAAGTTTCATCCCACGTTTCAACTTTCTAAGCATAGATCTGCCAGTGATTGTGGAGACATTTGTCAAGTA CCTGATTTCTCTTTCTGGAGCTCTGGCTATTGTTAATGCAGTGCCCTGCTTTGCCTTGGATGGTCAATGG ATTTTAAACTCTTTCCTGGATGCTACCCTTACTTCAGTAATTGGAGACAATGATGTCAAAGATCTGATTG GATTTTTCATCTTGCTTGGTGGCAGTGTACTTTTGGCTGCCAATGTGACACTGGGACTCTGGATGGTTAC AGCACGGTAATGTTTGCTCTCATCCAACAGAATTAAGAAGTAAAGCTACATGGCAATATCTGTTGCCATT GAAATTCTTACTAGTTATGAAATGTAAAGTGTTTCCTTAAAACACCTTGGCCAACCTCTTTGAGCTCCTA ATATCTAGAGAACCCAAAAACTCTGTAGTAGAGGGAGTAGAAGAAATGAAAAGCATAATTCCTGACTATA TCCTACTTTAAAGATTGAATGTGCAAACTCTGAATGTTTGTTGAATAATTTGTAAACAAGTGAAAATTCA TTCATGTAATACCATTTTGTGTGACCATATAGCATTTTGGAATAGTAAAAGGAAAAGCAGAATCTTGTAG GAAAACGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGAGAGAGAGAGAGAGAGAGAGAGAGA GAATGCGTGTGTATGTGGTATGTGTCTGCGTGTTCTACTAGTGTTTGAAAGCAGGGCCTTACTTATGCTA CTTAGGCTAAGCTTGCCTGTCCCAGGTAAAAGTTACTGAAAAGTGCCATTAAGGTGATGTGCTGGTGCAC ACCTATAATCCCAGCACCTACCTCTATGTGTTTGGAGGGCTAGGCATAAGGATCGTGAATTCAAGGCCAT CATCTTTAGCTACATAGTAGGTTCCAGACCAGCACTGGCCACATAGCAAGACCCTTTCGTGCATGCATAC ATGCACAGGCGCGCACACACACGCACCCACCCACCCACACACACACACACACACACACACACACACGCAC ATACACAAAATCATTAATGATTGGAAGGAAGCTAGACCTTGGTTGAATGGAAAGTAGAGCAAGTGCTCTT GGTTTTATAACTCTTGTCTTTATCTTATTTGAAAAGTATTTTAAAGTTTAATGGTGTATTATTCTGTACT CTGGTTACAATTCTATGAAAGTATCTGCTAACATGAAAGCAAAAACTCTGTACAAGTGCTATCAAGTAAA TGAGCAATGTTGAAAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:4460515 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4460515 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16314 same embryo
	EMAGE:16311 same embryo
	EMAGE:16312 same embryo
	EMAGE:16310 same embryo
	EMAGE:16313 same embryo
	EurExpress:euxassay_017176 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source