Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:16356

Tex10 testis expressed gene 10 ( MGI:1344413)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16356 EMAGE:16356 EMAGE:16356 EMAGE:16356 EMAGE:16356
"Pseudo-wholemount" of euxassay_017059. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_017059_01 euxassay_017059_02 euxassay_017059_03 euxassay_017059_04
EMAGE:16356 EMAGE:16356 EMAGE:16356 EMAGE:16356 EMAGE:16356
euxassay_017059_05 euxassay_017059_06 euxassay_017059_07 euxassay_017059_08 euxassay_017059_09
EMAGE:16356 EMAGE:16356 EMAGE:16356 EMAGE:16356 EMAGE:16356
euxassay_017059_10 euxassay_017059_11 euxassay_017059_12 euxassay_017059_13 euxassay_017059_14
EMAGE:16356 EMAGE:16356 EMAGE:16356 EMAGE:16356 EMAGE:16356
euxassay_017059_15 euxassay_017059_16 euxassay_017059_17 euxassay_017059_18 euxassay_017059_19
EMAGE:16356 EMAGE:16356 EMAGE:16356 EMAGE:16356
euxassay_017059_20 euxassay_017059_21 euxassay_017059_22 euxassay_017059_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16356Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16356_wholemount_strong.wlz
16356_wholemount_moderate.wlz
16356_wholemount_weak.wlz
16356_wholemount_possible.wlz
16356_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16356_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30787
Entity Detected:Tex10, testis expressed gene 10 ( MGI:1344413)
Sequence:sense strand is shown

>T30787
ACAGAGGGGCCTGCTTCTTCCAGTTAGGACTTTGTTAATCAAATTTTTCAGTAAGATCTATCAGAAAGAA
GAATTGAGGTCTTACAGATTGAGATACCGAAGCAAAGTGCTCTCCCGTTGGCTGGCCGGCTTGCCATTGC
AGCTCTCTTACCTTGGCTCCCGAAACCCTGAGCTCTCCACACAGCTGATTGATATTATTCACACTGCTGC
ATCACAAGCAAATAAGGACTTATTGAAAAACTTACAAGCTGCCGCTCTACGAATTTATGATCCTCAAGAA
GGCGCTGTGGTAGTTCTTCCTGCAGAGTCTCAGCAACGTCTAGTTCAGCTGTTGTATTTTCTCCCCACTC
TGCCTGCTGATTTGCTTTCTCAGCTAAGTCGGTGCTGCATAATGGGAAGACTCGGCTCAAGTTTGGCTGT
CACGCTTATTGGGATACTGTACATGAGATCATCTTTTTCTGGATGGAGTCACTCGGTTAATGACTGCTTA
ATGACTGATGTGGATTATCTCAGCTTCTTATTTTCTACACTTACAGGGTTTTCAAAAGAGGAGTTGACCT
GGCTTCAGAACCTTCGAGGCGTTCCTCATGTCGTCCAGACATCACTTTCCCCTGTGCTTCTCTACCTTAC
AGATTTAGACCAGTTCTTGCACCACTGGGACATAACAGAGGCAGTTTGTCACAGCTTATTGGTTATTCCT
GCCCGAAGCCAGAGCTTTGACATCATGCAGAGTGCCATCAGTAAGCATTTGGTTGGGTTGACTGTAATTC
CTGACAGCACAGCTGGCTGTATATTTGGAGTGATGTGTAAGCTCCTGGATCATACATGTGTAGTTAATGA
AGCTCTCCTGCCATTCCTGGCTTCTTGTTGCTACAGCCTTCTTTATTTTCTGCTTACTCTAGAGAAAGGA
GATGCAGAACATCTGAAAAAGAGGGACAAACTGTGGGAGGTCTGTGTCTCCATCCTGGCTCTCCTGCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3595167), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 16025. Forward Primer - name:016025_F_IRAV16-19_E22_Tex10, sequence:ACAGAGGGGCCTGCTTCT; Reverse Primer - name:016025_R_SP6_IRAV16-19_E22_Tex10, sequence:AGGCAGGAGAGCCAGGAT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16357 same embryo
 EMAGE:16355 same embryo
 EMAGE:16358 same embryo
 EMAGE:16359 same embryo
 EurExpress:euxassay_017059 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS