EMAGE mouse gene expression spatial database

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EMAGE:16377

Ank2 ankyrin 2, brain ( MGI:88025)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017190. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017190_01	euxassay_017190_02	euxassay_017190_03	euxassay_017190_04

euxassay_017190_05	euxassay_017190_06	euxassay_017190_07	euxassay_017190_08	euxassay_017190_09

euxassay_017190_10	euxassay_017190_11	euxassay_017190_12	euxassay_017190_13	euxassay_017190_14

euxassay_017190_15	euxassay_017190_16	euxassay_017190_17	euxassay_017190_18	euxassay_017190_19

euxassay_017190_20	euxassay_017190_21	euxassay_017190_22	euxassay_017190_23	euxassay_017190_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16377_wholemount_strong.wlz

16377_wholemount_moderate.wlz

16377_wholemount_weak.wlz

16377_wholemount_possible.wlz

16377_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16377_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8741
Entity Detected:	Ank2, ankyrin 2, brain ( MGI:88025)
Sequence:	sense strand is shown >T8741 AGATCCTGCAGCGCAGAAGCTAAATCTGTCACTGGGTCCCTATGGTAACATCTTACCTGCAGCAGATGGC CTGACTCTTGAATGGTAACACGGCCCTGGCGATTGCTAAGCGGCTGGGCTACATCTCCGTGGTTGACACC CTGAAGGTTGTGACTGAAGAGGTCACCACCACTACCACGACTATCACGGAAAAACATAAGCTAAATGTTC CTGAGACAATGACGGAGGTCCTTGATGTTTCTGATGAAGAGGGTGATGACACTGTGACAGGTGATGGGGG AGAATACCTCAGGCCAGAAGATCTCAAGGAGCTCGGAGATGACTCACTGCCCAGCAGTCAGTTCCTGGAT GGCATGAACTACCTTCGGTACAGTCTGGAGGGAGGAAGATCCGACAGCCTCCGGTCCTTCAGTTCCGACA GGTCTCACACTCTGAGCCATGCATCGTACCTGAGGGACAGTGCCATGATTGACGACACGGTTGTGATCCC CAGCCACCAGGTGTCTGCGCTAGCCAAGGAGGCAGAAAGGAATTCTTATCGTCTGAGCTGGGGCACTGAG AACTTAGACAACGTGGCTCTTTCTTCCAGTCCTATTCATTCAGGTTTCCTAGTCAGTTTTATGGTGGATG CCCGTGGTGGTGCCATGCGAGGATGCAGACACAATGGACTCAGAATCATTATCCCACCTCGGAAATGCAC AGCCCCAACTCGCGTCACCTGCCGCCTTGTGAAACGCCATCGACTGGCAACAATGCCGCCCATGGTGGAA GGAGAAGGCCTGGCCAGCCGCCTGATTGAAGTCGGACCTTCGGGAGCTCAGTTTCTTGGGCCCGTGATCG TGGAGATCCCTCACTTCGCCGCTCTTCGAGGAAAGGAGAGGGAGCTGGTGGTCCTGCGCAGTGAAAATGG GGACAGCTGGAAAGAGCATTTCTGCGACTACACTGAGGATGAATTGAACGAAATCCTTAATGGCATGGAT GAAGTGCTGGACAGTCCAGAAGACCTGGAAAAGAAACGAATCTGCCGCATCATCACTCGTGACTTCCCAC AGTACTTTGCGGTGGTGTCTCGCATCAAACAGGACAGCAACCTGATTGGCCCCGAGGGTGGAGTACTGAG CAGCACAGTGGTGTCACAGGTGCAAGCCGTCTTCCCAGAGGGCGCACTCACCAAGCGCATTCGAGTAGGC CTACAGGCTCAACCTATGCATAGTGAATTGGTAAAGAAGATCTTAGGCAACAAAGCCACCTTCAGCCCAA TAGTCACTTTGGAACCCAGGAGAAGAAAATTCCACAAGCCAATTACTATGACTATTCCTGTCCCCAAAGC TTCAAGTGATGTCATGCTGAATGGTTTTGGGGGAGACGCACCAACCTTAAGATTACTGTGCAGCATAACA GGTGGAACTACGCCTGCCCAGTGGGAAGACATCACAGGAACAACGCCACTAACATTTGTCAATGAGTGTG TTTCCTTTACAACCAACGTGTCTGCCAGGTTCTGGCTGATAGACTGTCGACAGATTCAGGAGTCTGTTGC CTTTGCATCACAAGTGTATAGAGAAATTATCTGTGTGCCATATATGGCCAAATTTGTAGTGTTTGCCAAG TCACATGACCCCATTGAAGCCAGGTTGCGGTGTTTCTGTATGACAGATGACAAAGTAGATAAGACCCTTG AACAACAAGAAAACTTCTCTGAGGTGGCCAGGAGCAGGGATGTGGAGGTATTGGAAGGAAAACCTATTTA TGTTGATTGTTTTGGCAACCTGGTTCCACTAACCAAGAGTGGCCAACATCATATATTCAGCTTTTTTGCC TTCAAAGAAAATAGACTTCCTCTCTTTGTCAAGGTTCGTGACACAACCCAGGAACCTTGCGGGCGACTCT CATTTATGAAGGAACCCAAATCCACAAGAGGACTGGTGCATCAAGCTATTTGCAACTTAAACATCACCCT GCCAATTTATGCCAAGGAATCGGAGTCAGATCAAGAGCCGGAGGAAGAGATCGGTATGACATCCGAAAAA AATCCCCAAGATGAGCAGGAGCGGATGGAAGAAAGGCTGGCTTACATCGCTGATCACCTTGGCTTCAGCT GGACAGAATTAGCAAGAGAACTGGATTTCACTGAGGAGCAAATTCACCAAATTCGAATCGAGAACCCCAA CTCCCTTCAAGATCAGAGCCACGCACTGCTCAAGTACTGGCTGGAGAGGGATGGGAAGCATGCCACAGAT ACCATCCTCATCGAATGCCTCACCAAGATCAACAGGATGGACATTGTACATCTCCTGGAGACCAACACAG AGCCCCTCCAGGAGCGCATGGGCCGCAGCTATGCAGAAATAGAGCAGACCATTACGCTGGACCACAGTGA AGGATTTTCAGTCCTTCCAGACGAGCTCTGTGCTGCCAAGGAGAAGAAGGAGCAGGAAGCTTCCAAAGAA AGCGAGTCTAGCGACCACCCGCCCATGGTCTCCGAAGAAGACATATCTGTCGGTTATTCCACATTTCAGG ATTGCCTCCCCAAAACTGAAGGGGACAGCCCAGCAGCAGCACTGTCTCCTCAAATGCACCAGGAGCCAGT TCAACAAGATTTCTCAGGGAAAACGCAAGACCAGCAGGAATATTATGTAACAACACCAGGGGCAGAAGTG GAAGACCCTCAGAAGGCCACAGCCGTTCCTGACTCTCTCTGTAAGACTCCTGAGGACATCAGTACCCCTC CTGAGGGAACAAAGCCTTGTCTCCAGACCCCGGTGACTAGCGAACGTGGTTCTCCGATTGTGCAAGAACC TGAGGAGGCTTCCGAGCCCAAAGAGGAGAGTTCTCCAAGGAAAACTAGCCTGGTCATTGTTGAGTCAACA GACGACCAGTCTCAGGTCTTTGAAAGACTGGATGGTGATGCCGCTTTTCAAAAGGGAGACGATATGCCTG ACATACCCCCAGAGACGGTCACAGAGGAAGAATATGTTGATGAGAATGGACACACCGTGGTGAAGAAGGT TACCCGGAAAATCATTAGGCGGTACGTTTCCTCTGATGGCACAGAGAAGGAGGAGGTTACCATGCAGGGA ATGCCTCAGGAGCCAGTCAACATTGAGGATGGGGACAATTATTCCAAAGTGATAAAGCGCGTGGTATTGA AGAGTGACACCCAGCAGTCAGAGGTGACTTTGTCTGAACCCAGCGTTTTGTCCAGTACCTCACAGTTTCA GGCCGAGCCAGTAGAAGGCCGTAGAGTCAGCAAAGTTGTTAAAACAACCATGGTACACGGAGAACGGATG GAGAAGAGTCTTGGGGACTCTAGCTTAGCCACTGACCTTCCTTCAGCCAAAGATGACTTTGAAGAGGACA ACAATGAGTAAAGCCAGGACACAGAGGAGGGCTGTGGTGAAGGACCAGCAGGGGAAACACATCACCTTGG AGCACCTGGAGGATGTTCCCGGAGCACTGGACCAAGACGACCTCCAGCGAGATCTCCAGCAACTCCTTCG GCACTTCTGCAAGGAGAACACAAAGCAAGAGGCCAAATGAGGAGCTGCCCAGTTCTCACCAGAAACCACA AACCCACTCAATATGCAACTTCCCATTTCTGCGCAGGAGTGACTGAAGTGGCCTAATTACTGGGACCCAG TGACACTTCCACTCTCAGCAAACACACAGCATCTACTTTTCCTTCTTTCTCCCTGCCCCTCTCTAACTAT AAGCTAATTTGTGACCAAAGATAGCATCCTTCCTTCCGGATGCTGTATCCACTATTTTGAGCATCCGTGC TAACCTGGGAACTGGCATCTCTATTGTTTGCTTCTGCTCAAGCTCCATGAAACTCCATGAATCAGAAGAA CTTCACCTGCAGACTTCTTCAAATGACAGGGGAATCCTTCCAAGGGTATCTATGTATAATGTATAGCCGA AATTCTCAGACGAACAACAGAGTAAAATTCAAACCACTGCCTGTCATAATTACACTGGGCATCATGGAAT AAAAGCCTCTAGATATTGCTGAACAATGGTTAATTATGATATTGCTAACACAATCAAATGGTAATACAGT TCTATGGAAGAAACACTTCAGACTTAGACACGTCCTTAGAACTTCCAGAGTAGCCATTTGCTCTTAAAGC CGTCAGGATCATAATCTTGAAGAAGAATCCCTAGGAGTGGTCTCTAGGACTGGTCAGCCATAGCCGTTGG CTCTTGGACTGTGGCCCTGAGGGAGAGGGAGAAGGACTCACAGGCCAGCAGATGGAAACAGCGAGGTATT CTGGTGCTAGAAGCTTCAGGGGTGGTGTTCTAGCACCTTCAGGTGTTTTTGACTTTGGACACGTTGGCAG GGTATTTTAAAAGCTATAACTACTGTAGTTTTCCGGTGTTCATTGCTGCTTTAGCAAACCACGCTGTCTT ACTGGTGGTACTTTCTTCTGGCCACTGCACTGTAGATAATTCATTGGAAACAAGATTTATGCACCACACA AAAGGTTAAATGCTGTCACCATGTTGGAGTGGTTTCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTCTTTT CTTTTCTTTTCTTTTTCCCCACCTCCAAGTTTATCTTTCCTCTAATACCTGCATGTAGCATCACACACAC ACACACACACACACACACACACACACACACACACACATCTTCTCATTACACTAATGGTTTTCACTCTGAG TCTCAACTTTCTCGCCATGTCTGCTCTTCAGCTTGCAGATGAGATTTTGGGAAATCCTTGGAAAATTTGG TTCTCATTAATTTTTTTTTTTGGTTTATTTATTCAAACCCCAGTCACTCTTGGAAACTGAAGTGCATTTG TGTGACTTTCTGTTTGTTTTAGAGAAGGGACAATGACAGTCTGAGTGCTCCCCATGCCCTATGTCGAATC CCCCTAGTGATTGAAGCTGTGTAGCATTTTAACATATATATATAAATATATTCACAAATATATTCATATA AAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6405358 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6405358 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16379 same embryo
	EMAGE:16376 same embryo
	EMAGE:16378 same embryo
	EMAGE:16375 same embryo
	EMAGE:16374 same embryo
	EurExpress:euxassay_017190 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source