Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:16382

Mir695 microRNA 695 ( MGI:3629632)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16382 EMAGE:16382 EMAGE:16382 EMAGE:16382 EMAGE:16382
"Pseudo-wholemount" of euxassay_019159. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019159_01 euxassay_019159_02 euxassay_019159_03 euxassay_019159_04
EMAGE:16382 EMAGE:16382 EMAGE:16382 EMAGE:16382 EMAGE:16382
euxassay_019159_05 euxassay_019159_06 euxassay_019159_07 euxassay_019159_08 euxassay_019159_09
EMAGE:16382 EMAGE:16382 EMAGE:16382 EMAGE:16382 EMAGE:16382
euxassay_019159_10 euxassay_019159_11 euxassay_019159_12 euxassay_019159_13 euxassay_019159_14
EMAGE:16382 EMAGE:16382 EMAGE:16382 EMAGE:16382 EMAGE:16382
euxassay_019159_15 euxassay_019159_16 euxassay_019159_17 euxassay_019159_18 euxassay_019159_19
EMAGE:16382 EMAGE:16382 EMAGE:16382 EMAGE:16382 EMAGE:16382
euxassay_019159_20 euxassay_019159_21 euxassay_019159_22 euxassay_019159_23 euxassay_019159_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16382Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16382_wholemount_strong.wlz
16382_wholemount_moderate.wlz
16382_wholemount_weak.wlz
16382_wholemount_possible.wlz
16382_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16382_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T70145
Entity Detected:Mir695, microRNA 695 ( MGI:3629632)
Sequence:sense strand is shown

>T70145
AGATTGGGCATAGGTGACTGAA
Notes:The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-695 was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:LNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16384 same embryo
 EMAGE:16383 same embryo
 EMAGE:16386 same embryo
 EMAGE:16385 same embryo
 EurExpress:euxassay_019159 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS