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EMAGE:16456

Mir99a microRNA 99a ( MGI:2676912)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16456 EMAGE:16456 EMAGE:16456 EMAGE:16456 EMAGE:16456
"Pseudo-wholemount" of euxassay_019106. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019106_01 euxassay_019106_02 euxassay_019106_03 euxassay_019106_04
EMAGE:16456 EMAGE:16456 EMAGE:16456 EMAGE:16456 EMAGE:16456
euxassay_019106_05 euxassay_019106_06 euxassay_019106_07 euxassay_019106_08 euxassay_019106_09
EMAGE:16456 EMAGE:16456 EMAGE:16456 EMAGE:16456 EMAGE:16456
euxassay_019106_10 euxassay_019106_11 euxassay_019106_12 euxassay_019106_13 euxassay_019106_14
EMAGE:16456 EMAGE:16456 EMAGE:16456 EMAGE:16456 EMAGE:16456
euxassay_019106_15 euxassay_019106_16 euxassay_019106_17 euxassay_019106_18 euxassay_019106_19
EMAGE:16456 EMAGE:16456
euxassay_019106_20 euxassay_019106_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16456Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16456_wholemount_strong.wlz
16456_wholemount_moderate.wlz
16456_wholemount_weak.wlz
16456_wholemount_possible.wlz
16456_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16456_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T70222
Entity Detected:Mir99a, microRNA 99a ( MGI:2676912)
Sequence:sense strand is shown

>T70222
AACCCGTAGATCCGATCTTGTG
Notes:The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-99a was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:LNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16455 same embryo
 EMAGE:16457 same embryo
 EurExpress:euxassay_019106 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS