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EMAGE:16550

Shank2 SH3/ankyrin domain gene 2 ( MGI:2671987)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16550 EMAGE:16550 EMAGE:16550 EMAGE:16550 EMAGE:16550
euxassay_016729_01 euxassay_016729_02 euxassay_016729_03 euxassay_016729_04 euxassay_016729_05
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euxassay_016729_06 euxassay_016729_07 euxassay_016729_08 euxassay_016729_09 euxassay_016729_10
EMAGE:16550 EMAGE:16550 EMAGE:16550 EMAGE:16550 EMAGE:16550
euxassay_016729_11 euxassay_016729_12 euxassay_016729_13 euxassay_016729_14 euxassay_016729_15
EMAGE:16550 EMAGE:16550 EMAGE:16550 EMAGE:16550 EMAGE:16550
euxassay_016729_16 euxassay_016729_17 euxassay_016729_18 euxassay_016729_19 euxassay_016729_20
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euxassay_016729_21 euxassay_016729_22 euxassay_016729_23 euxassay_016729_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16550Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16550_wholemount_strong.wlz
16550_wholemount_moderate.wlz
16550_wholemount_weak.wlz
16550_wholemount_possible.wlz
16550_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16550_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38002
Entity Detected:Shank2, SH3/ankyrin domain gene 2 ( MGI:2671987)
Sequence:sense strand is shown

>T38002
GTGGACTTGGATGAGGACTTTCTTTTCACAGAACCGTTGCCTCCCCCCCTGGAATTCGCCAATAGTTTTG
ATATCCCCGATGACCGGGCAGCTTCAGTTCCCGCTCTGGCTGACCTGGTCAAGCAGAAGAAAAACGACAC
CTCTCAGCCCCCTACGTTGAACTCCAGCCAACCAGCCAACTCCACAGACAGTAAGAAGCCAGCCGGTATC
TCGAACTGTCTGCCCTCCTCGTTCCTGCCACCCCCCGAAAGTTTCGATGCAGTCACCGACTCGGGGATTG
AGGAGGTGGACAGCCGGAGTAGCAGCGACCACCACCTGGAGACTACCAGCACCATCTCCACGGTGTCCAG
CATCTCCACGCTGTCCTCAGAGGGCGGCGAGAGCATGGACACCTGCACAGTCTATGCAGACGGGCAAGCC
TTTGTGGTTGACAAGCCCCCAGTACCTCCAAAGCCAAAAATGAAGCCCATCGTTCACAAGAGCAACGCAC
TTTACCAAGACACGCTCCCAGAAGAGGACACGGATGGCTTTGTGATCCCCCCACCTGCACCCCCGCCCCC
GCCGGGCAGCGCCCAGGCCGGTGTGGCGAAGGTCATCCAGCCAAGGACCTCCAAGTTGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 80848. Forward Primer - name:080848_F_cDNA_Shank2, sequence:GTGGACTTGGATGAGGACTTTC; Reverse Primer - name:080848_N_SP6_cDNA_Shank2, sequence:ACAACTTGGAGGTCCTTGGCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16553 same embryo
 EMAGE:16552 same embryo
 EMAGE:16549 same embryo
 EMAGE:16551 same embryo
 EMAGE:16548 same embryo
 EurExpress:euxassay_016729 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS