Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:16626

Stx6 syntaxin 6 ( MGI:1926235)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16626 EMAGE:16626 EMAGE:16626 EMAGE:16626 EMAGE:16626
"Pseudo-wholemount" of euxassay_016830. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_016830_01 euxassay_016830_02 euxassay_016830_03 euxassay_016830_04
EMAGE:16626 EMAGE:16626 EMAGE:16626 EMAGE:16626 EMAGE:16626
euxassay_016830_05 euxassay_016830_06 euxassay_016830_07 euxassay_016830_08 euxassay_016830_09
EMAGE:16626 EMAGE:16626 EMAGE:16626 EMAGE:16626 EMAGE:16626
euxassay_016830_10 euxassay_016830_11 euxassay_016830_12 euxassay_016830_13 euxassay_016830_14
EMAGE:16626 EMAGE:16626 EMAGE:16626 EMAGE:16626 EMAGE:16626
euxassay_016830_15 euxassay_016830_16 euxassay_016830_17 euxassay_016830_18 euxassay_016830_19
EMAGE:16626
euxassay_016830_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16626Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16626_wholemount_strong.wlz
16626_wholemount_moderate.wlz
16626_wholemount_weak.wlz
16626_wholemount_possible.wlz
16626_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16626_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T63178
Entity Detected:Stx6, syntaxin 6 ( MGI:1926235)
Sequence:sense strand is shown

>T63178
CACTCAGTCTCGACTGGACAACGTGATGAAGAAACTTGCAAAAGTGTCTCACATGACCAGTGATCGGCGC
CAGTGGTGTGCCATAGCCATCCTCTTCGCGGTCCTGGTGGTCGTGCTGATCCTCTTCCTAGTGCTGTGAT
GGTGGCCCGCCCGCCTCAGGCACTTCTGCACGAGGACCCAGGGGAGAGGAGAAGCAAGCACTCCGCCACT
TACATTCCTCGCCCAGAAACACACCTCTACCCCGCCTCCTCTGTGACTCCAGCTTTGAGACAGCCCTCTC
TCCCAGCGCTCAAAGGGCAAGCTTCGAAGAGGGAAGAGGTGACCATGTGCCCGGCCGGATAGCAGAAGCC
CCGGCTGCCCACTCTTGCTGAGGACAGCAAGCCACTGCTTTGCCTTACTGGACCTCGCTCCTTGAGGAAG
GAAGGACAGAGTCCTCAGAAGAACTGACAGCCCCACTTACTCCGTTGCTCATTCTGCTGGTCTCTGGCAG
CCTTTCCTTCTACTCAGACCCTTTTCCCTGCTTAGATGGGTTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 178431. Forward Primer - name:178431_F_cDNA_Stx6, sequence:CACTCAGTCTCGACTGGACAAC; Reverse Primer - name:178431_N_SP6_cDNA_Stx6, sequence:CAACCCATCTAAGCAGGGAAA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16623 same embryo
 EMAGE:16622 same embryo
 EMAGE:16621 same embryo
 EMAGE:16624 same embryo
 EMAGE:16625 same embryo
 EurExpress:euxassay_016830 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS